Investigation of Skin Irritation of Essential Oils from Interior-warming Medicines

Yun-fei DONG; Feng-ye WU; Jiang-an XI; Han FENG; Yong-kun WEN; Jun-hong YAO; Xue-min ZHU; Jie DONG; Ye-qing QU; Jun CHEN

doi:10.13422/j.cnki.syfjx.20190548

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Investigation of Skin Irritation of Essential Oils from Interior-warming Medicines

Pharmacy and Processing | 更新时间：2021-02-09

- Investigation of Skin Irritation of Essential Oils from Interior-warming Medicines
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 156-161(2019)
- 作者机构：
  
  南京中医药大学　药学院，第一临床医学院，卫生经济管理学院，江苏省中药药效与安全性评价重点实验室，鼓楼中西医结合临床医学院，南京　 210023
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190548
  CLC： R22; R24; R28; C94; TS193
- Published：20 July 2019，
  
  Published Online：19 November 2018，
  
  Received：01 October 2018，
- 稿件说明：
扫描看全文
Yun-fei DONG, Feng-ye WU, Jiang-an XI, et al. Investigation of Skin Irritation of Essential Oils from Interior-warming Medicines. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):156-161(2019)
DOI：

Yun-fei DONG, Feng-ye WU, Jiang-an XI, et al. Investigation of Skin Irritation of Essential Oils from Interior-warming Medicines. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):156-161(2019) DOI： 10.13422/j.cnki.syfjx.20190548.

摘要

目的：

考察温里药挥发油的皮肤刺激性。

方法：

以3种温里药挥发油(肉桂挥发油、丁香挥发油、高良姜挥发油)为研究对象，比较三者的皮肤细胞毒性和在体皮肤刺激性，并利用皮肤测试板预测这3种中药挥发油皮肤刺激性。

结果：

3种中药挥发油对人皮肤成纤维细胞(HSF)的毒性相差显著，肉桂挥发油、高良姜挥发油、丁香挥发油的半数抑制率(IC

)分别为(11.16±0.28)，(53.33±1.71)，(226.70±17.61) mg·L

－1

；在体皮肤刺激性评价表明，3种温里药挥发油对皮肤刺激性的排序为肉桂挥发油

丁香挥发油

高良姜挥发油；皮肤测试板评价3种挥发油刺激性结果与在体皮肤刺激性结果一致。

结论：

肉桂挥发油、丁香挥发油以及高良姜挥发油的皮肤细胞毒性评价结果与其在体皮肤刺激性结果不一致，采用皮肤测试板有望能够准确预测挥发油的在体皮肤刺激性。

Abstract

Objective:

To investigate the skin irritation of essential oils(EOs) extracted from interior-warming medicines.

Method:

Three EOs from interior-warming medicines(Cinnamomi Cortex

Caryophylli Flos and Alpiniae Officinarum Rhizoma) were selected as research objects.The

in vitro

skin cytotoxicity and

in vivo

skin irritation of these EOs were determined and compared.Moreover

the skin irritation was also predicted by the novel skin test panels.

Result:

Toxicity of these three EOs to human skin fibroblasts(HSF) was significantly different

half-inhibitory concentration(IC

) values of EOs from Cinnamomi Cortex

Alpiniae Officinarum Rhizoma and Caryophylli Flos were (11.16±0.28)

(53.33±1.71)

(226.70±17.61) mg·L

-1

respectively.However

in vivo

skin irritation evaluation showed that the local toxicity of these three EOs was in the order of EO of Cinnamomi Cortex

EO of Caryophylli Flos

EO of Alpiniae Officinarum Rhizoma. The evaluation results of skin test panels for these three EOs were in accordance with the results of

in vivo

skin irritation evaluation.

Conclusion:

Toxicity of these three EOs against skin cells

in vitro

is inconsistent with their

in vivo

skin irritation. Skin test panels are expected to be able to accurately predict

in vivo

skin irritation of EOs instead of cytotoxicity evaluation.

关键词

温里药挥发油皮肤细胞毒性在体皮肤刺激性皮肤测试板半数抑制率罗丹明B

Keywords

interior-warming medicinesessential oilsskin cytotoxicityin vivo skin irritationskin test panelshalf-inhibitory concentrationrhodamine B

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