Advances in Bioculture Technology of Bupleuri Radix and Biosynthesis Pathways of Saikosaponins

Chen WANG; Li-cheng AN; Jian-chao LI; Wen-tao QI; Chang-li LIU; Li LI

doi:10.13422/j.cnki.syfjx.20191048

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Advances in Bioculture Technology of Bupleuri Radix and Biosynthesis Pathways of Saikosaponins

Review | 更新时间：2021-02-09

- Advances in Bioculture Technology of Bupleuri Radix and Biosynthesis Pathways of Saikosaponins
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 228-234(2019)
- 作者机构：
  
  1.首都医科大学　中医药学院，北京　 100069；
  2.中医络病研究北京重点实验室，北京　 100069；
  3.北京市中药研究所，北京　 100035；
  4.首都医科大学　附属北京中医医院，北京　 100010
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191048
  CLC： R22; R931; R28; Q52; Q813.1
- Published：20 July 2019，
  
  Published Online：25 January 2019，
  
  Received：30 November 2018，
- 稿件说明：
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Chen WANG, Li-cheng AN, Jian-chao LI, et al. Advances in Bioculture Technology of Bupleuri Radix and Biosynthesis Pathways of Saikosaponins. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):228-234(2019)
DOI：

Chen WANG, Li-cheng AN, Jian-chao LI, et al. Advances in Bioculture Technology of Bupleuri Radix and Biosynthesis Pathways of Saikosaponins. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):228-234(2019) DOI： 10.13422/j.cnki.syfjx.20191048.

摘要

合成生物学是解析中药活性成分生物合成途径、发掘参与生物合成功能基因的一门新兴学科。柴胡是我国常用大宗中药材，药用价值显著。柴胡皂苷是其指标性成分，具有抗炎、抗病毒、抗肿瘤等显著活性。但目前野生柴胡资源遭到破坏，人工栽培过程中存在一定问题。生物培养技术及合成生物学的应用可扩大柴胡皂苷的来源，保护柴胡资源。不同植物的培养条件没有固定模式可遵循，不同药用活性成分的生物合成途径也是不一致的。目前，尚无柴胡组织培养技术及柴胡皂苷生物合成途径研究的综述报道。该文拟介绍合成生物学过程中用到的愈伤组织培养、不定根培养、毛状根培养与悬浮细胞培养等生物培养技术与柴胡皂苷生物合成途径及其关键酶功能基因的研究进展，建议通过借鉴其他传统根类药材的组织培养技术，对柴胡的生物培养技术进行优化，为深入研究柴胡皂苷的生物合成途径与代谢调控提供参考。

Abstract

Synthetic biology is an emerging discipline that analyzes the biosynthesis pathways of active constituents in traditional Chinese medicine and explores genes involved in biosynthesis. Bupleuri Radix is one of the most commonly used Chinese medicinal materials with remarkable medicinal value

its index component is saikosaponins

which has significant anti-inflammatory

anti-viral and anti-tumor activities. However

the current wild resources of Bupleuri Radix have been destroyed

and there were some problems in the process of artificial cultivation. The application of biological culture technology and synthetic biology can expand the sources of saikosaponins and protect resources of Bupleuri Radix. The culture conditions of different plants can be followed without a fixed pattern

and the biosynthetic pathways of different medicinal active ingredients are also inconsistent. At present

there is no review report on the culture technology of Bupleuri Radix and the research on the biosynthesis pathway of saikosaponins. This paper introduces the research progress of biological culture techniques

such as callus culture

adventitious root culture

hairy root culture and suspension cell culture used in synthetic biology

and the biosynthesis pathway of saikosaponins and its key enzyme functional genes. It is suggested to optimize the biological culture technology of Bupleuri Radix by referring to the tissue culture technology of other traditional root medicinal materials

so as to provide a reference for the in-depth study on the biosynthesis pathway and metabolic regulation of saikosaponins.

关键词

柴胡生物培养技术柴胡皂苷合成生物学不定根培养条件组学

Keywords

Bupleuri Radixbiological culture techniquesaikosaponinssynthetic biologyadventitious rootsculture conditionsomics

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