Inhibitory Effect of Zeqi Tang on Mouse Model of Lung Cancer

Zi-hang XU; Yang-zhuang-zhuang ZHU; Fei ZHANG; Lu-yao WEI; Lin SU; Xiao-ning JIAO; Qin ZHOU; Ning ZHANG; Zhen-zhen HUANG; Xian-dan ZHU; Fei LIU; Hai-rong ZHONG; Shi-guo ZHU; Xiao CHEN; Chun-pu ZOU

doi:10.13422/j.cnki.syfjx.20191322

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Inhibitory Effect of Zeqi Tang on Mouse Model of Lung Cancer

Research on Anti-lung Cancer Effect of TCM Compound and Its Effective Components | 更新时间：2021-02-09

- Inhibitory Effect of Zeqi Tang on Mouse Model of Lung Cancer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 14, Pages: 6-12(2019)
- 作者机构：
  
  1.上海中医药大学　基础医学院，经方理论应用研究中心，中药与系统生物学中心，科技实验中心，上海　 201203；
  2.上海交通大学　医学院　附属新华医院，上海　 200092
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191322
  CLC： R22; R242; R2-031; R285.5
- Published：20 July 2019，
  
  Published Online：20 March 2019，
  
  Received：06 March 2019，
- 稿件说明：
扫描看全文
Zi-hang XU, Yang-zhuang-zhuang ZHU, Fei ZHANG, et al. Inhibitory Effect of Zeqi Tang on Mouse Model of Lung Cancer. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):6-12(2019)
DOI：

Zi-hang XU, Yang-zhuang-zhuang ZHU, Fei ZHANG, et al. Inhibitory Effect of Zeqi Tang on Mouse Model of Lung Cancer. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(14):6-12(2019) DOI： 10.13422/j.cnki.syfjx.20191322.

摘要

目的：

建立小鼠肺癌原位模型，用泽漆汤干预模型小鼠以观其抑瘤功效，并探索潜在作用机制。

方法：

通过肺内注射1×10

个LLC-luc细胞建立非小细胞肺癌原位小鼠模型，用泽漆汤(0.171 g·mL

－1

)或生理盐水对模型小鼠进行每日灌胃，连续35 d，观察小鼠的外观表现(精神、毛发、食欲等)，生存期及泽漆汤抑瘤效果；每周活体成像检测小鼠肺内荧光信号强弱。流式细胞术检测模型小鼠脾脏中NK细胞数量，CD107

实验检测泽漆汤用药后小鼠脾脏中NK细胞脱颗粒情况。采用Kromasil 1005 C

色谱柱，以0.025%磷酸-乙腈梯度洗脱，流速1.0 mL·min

－1

，柱温35 ℃，检测波长265 nm，建立泽漆汤指纹图谱并采用中国药典委员会推荐的中药色谱指纹图谱相似度评价系统软件(2012版)对10批样品指纹图谱进行评价以完成对泽漆汤的质量控制。

结果：

泽漆汤能够明显抑制肺癌原位模型小鼠肺部荧光信号(

0.05

0.01)，延长小鼠生存期(

0.01)；泽漆汤干预后小鼠体内肿瘤中NK细胞显著增多(

0.01)，CD107

脱颗粒也显著增加(

0.01)。并且，对泽漆汤指纹图谱的相似度评价结果显示，10批泽漆汤水提液的指纹图谱相似度均≥0.9，表明不同批次间差异较小。

结论：

泽漆汤可能通过上调小鼠体内NK细胞数量，增强其脱颗粒能力，从而达到抑制小鼠体内肿瘤生长，延长其生存期的作用。泽漆汤HPLC指纹图谱相似度评价可反映泽漆汤质量的稳定性，为后续研究提供用药基础。

Abstract

Objective:

To observe effect of Zeqi Tang in intervening mice with orthotopic lung cancer model

in order to observe its anti-tumor mechanism.

Method:

An in situ mouse model of non-small cell lung cancer was established through intrapulmonary injection with 1×10

LLC-luc cells. The model mice were intragastrically administered with Zeqi Tang(0.171 g·mL

-1

) or normal saline for 35 days. Appearance (spirit

hair

appetite

sleep)

survival period and Zeqi Tang anti-tumor effect were observed

weekly vital imaging was performed to detect the fluorescence signal in the lungs of mice. Flow cytometry was used to detect the NK cell content in the spleen of the model mice. CD107

was used to detect the degranulation of NK cells in the spleen of mice after administration of Zeqi Tang. Kromasil 100 5 C

column was used and eluted with acetonitrile-0.025%phosphoric acid in a gradient mode

with flow rate at 1.0 mL·min

-1

column temperature at 35 ℃ and detection wavelength of 265 nm

as to establish the fingerprint of Zeqi Tang. The fingerprints of 10 batches of samples was evaluated by using the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System Software (2012 Edition) recommended by the Chinese Pharmacopoeia Commission

in order to complete the quality control of Zeqi Tang.

Result:

Zeqi Tang could significantly inhibit the lung fluorescence signal of lung cancer in situ model mice and prolong the survival of mice(

0.05

0.01). After the intervention with Zeqi Tang

the NK cells in the tumors increased significantly(

0.01)

and the degranulation of CD107

also increased significantly(

0.01). Moreover

the HPLC-DAD fingerprint of Zeqi Tang showed a significant increase in the fingerprint similarity of 10 batches of lacquer soup aqueous extract. Moreover

the HPLC-DAD fingerprint of Zeqi Tang showed that the fingerprint similarity of 10 batches of lacquer soup aqueous extract was ≥0.9

indicating that small differences between the batches.

Conclusion:

Zeqi Tang may enhance the tumor growth and prolong the survival period of mice by up-regulating the number of NK cells in mice and enhancing their degranulation function. The evaluation of similarity of HPLC fingerprint of Zeqi Tang reflects the quality of lacquer soup to a certain extent

and can provide reference for further study.

关键词

泽漆汤肺癌小鼠模型NK细胞质量评价

Keywords

Zeqi Tangpulmonary mouse modelNK cellsquality evaluation

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