Optimization of Electromagnetic Cracking Extraction Technology of Baicalin in Scutellariae Radix by Response Surface Methodology

Ren-guang WANG; Ai-ling JIA; Zhi-dong QIU; Jing-yao YANG; Xin-shu ZHANG; Ji-qi YANG

doi:10.13422/j.cnki.syfjx.20191349

您当前的位置：

首页 >

文章列表页 >

Optimization of Electromagnetic Cracking Extraction Technology of Baicalin in Scutellariae Radix by Response Surface Methodology

Pharmacy and Processing | 更新时间：2021-02-09

- Optimization of Electromagnetic Cracking Extraction Technology of Baicalin in Scutellariae Radix by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 21, Pages: 106-111(2019)
- 作者机构：
  
  1.长春中医药大学　中药有效成分省部共建教育部重点实验室，长春　 130117；
  2.成都中医药大学　药学院，成都　 611137
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191349
  CLC： R22; R94; R28; C37; O657.7
- Published：05 November 2019，
  
  Published Online：19 March 2019，
  
  Received：23 January 2019，
- 稿件说明：
扫描看全文
Ren-guang WANG, Ai-ling JIA, Zhi-dong QIU, et al. Optimization of Electromagnetic Cracking Extraction Technology of Baicalin in Scutellariae Radix by Response Surface Methodology. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):106-111(2019)
DOI：

Ren-guang WANG, Ai-ling JIA, Zhi-dong QIU, et al. Optimization of Electromagnetic Cracking Extraction Technology of Baicalin in Scutellariae Radix by Response Surface Methodology. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):106-111(2019) DOI： 10.13422/j.cnki.syfjx.20191349.

摘要

目的：

建立电磁裂解水提黄芩中黄芩苷的最佳工艺条件，为该提取技术的推广与应用提供参考。

方法：

在单因素试验基础上，选取提取时间、物料粒度、液料比3个因素进行Box-Behnken中心组合设计，以黄芩苷提取率为指标，利用响应面法优化黄芩苷电磁裂解提取的最佳工艺参数，并与超声法、乙醇回流法和煎煮法进行比较。

结果：

最佳工艺条件为提取时间2.41 min，物料粒度过100目筛，液料比33 mL·g

－1

；在该条件下，黄芩苷提取率12.21%。运用乙醇回流法、煎煮法和超声法提取时，黄芩苷的提取率分别为12.91%，12.62%，11.61%。电磁裂解法提取黄芩苷的得率与其他几种常规提取方法接近，但提取时间明显缩短。

结论：

作为一种新兴的中药提取技术，电磁裂解提取具有快速高效、省时节能、绿色环保等优点，可为黄芩苷的工业化提取提供新方法。

Abstract

Objective:

To optimize the extraction conditions of baicalin from Scutellariae Radix by electromagnetic pyrolysis.

Method:

Based on single factor experiments

taking extraction time

material size and liquid-material ratio as factors

yield of baicalin as index

extraction parameters were optimized by response surface methodology

and compared with ultrasonic method

ethanol refluxing method and decoction method.

Result:

The optimal extraction conditions were as follows: extraction time of 2.41 min

material size of 100 mesh

liquid-material ratio of 33 mL·g

-1

. Under these conditions

the yield of baicalin was 12.21%. The yields of baicalin by ethanol refluxing method

decoction method and ultrasonic method were 12.91%

12.62%and 11.61%

respectively. The yield of baicalin by electromagnetic cracking extraction was close to several other conventional extraction methods

and the extraction time was significantly shortened.

Conclusion:

As a novel extraction technology of traditional Chinese medicine

electromagnetic cracking extraction has the advantages of high efficiency

energy and time saving

green environmental protection

etc. And it can provide a new method for the industrial extraction of baicalin.

关键词

黄芩电磁裂解法响应面法黄芩苷提取工艺扫描电子显微镜物料粒度

Keywords

Scutellariae Radixelectromagnetic cracking methodresponse surface methodologybaicalinextraction technologyscanning electron microscopematerial size

references

郑勇凤，王佳婧，傅超美，等．黄芩的化学成分与药理作用研究进展[J]．中成药，2016，38(1)：141-144．

徐丹洋，陈佩东，张丽，等．黄芩的化学成分研究[J]．中国实验方剂学杂志，2011，17(1)：78-80．

金鹏，许海舰，徐宝欣，等．黄芩苷研究现状及其镁盐研究前景[J]．中国实验方剂学杂志，2017，23(20)：228-234．

张喜平，田华，程琪辉．黄芩苷的药理作用研究现状[J]．中国药理学通报，2003，19(11)：1212-1214．

Kim E H, Shim B, KANG S, et al. Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules [J].J Ethnopharmacol, 2009, 126(2): 320-331.

GUO Q L, ZHAO L, YOU Q D, et al. Anti-hepatitis B virus activity of wogonin in vitro and in vivo [J].Antiviral Res, 2007, 74(1): 16-24.

史亚军，刘剑云，杨明．黄芩不同提取物溶出度和抗氧化性能评价[J]．中国实验方剂学杂志，2014，20(21)：1-4．

蒋萍，杨阳，季莉莉，等．黄芩素对4种肝损伤物质造成肝细胞毒性的拮抗作用[J]．中国实验方剂学杂志，2012，18(6)：145-147．

Woo K J, Lim J H, Suh S I, et al. Differential inhibitory effects of baicalein and baicalin on LPS-induced cyclooxygenase-2 expression through inhibition of C/EBPβ DNA-binding activity [J].Immunobiology, 2006, 211(5): 359-368.

PENG X D, DAI L L, HUANG C Q, et al. Correlation between anti-fibrotic effect of baicalin and serum cytokines in rat hepatic fibrosis [J].World J Gastroenterol, 2009, 15(37): 4720-4725.

WANG H, MA X D, MA Q B, et al. Deep eutectic solvent-based ultrahigh pressure extraction of baicalin from Scutellaria baicalensis Georgi. [J].Molecules, 2018, 23(12): 1420-3049.

叶陈丽，贺帅，张守尧，等．Box-Behnken设计优化选黄芩超高压提取工艺[J]．中国实验方剂学杂志，2013，19(3)：40-44．

石磊，张振巍．二次回归正交设计法优选黄芩苷醇提工艺[J]．中药材，2011，34(12)：1946-1950．

高卫华，李静，杨翓，等．正交试验法优选黄芩煎煮工艺[J]．北京中医药大学学报，1998，21(4)：41-42．

王佩琪，王成明，卢静华．正交试验法优选超声提取黄芩苷的研究[J]．中华中医药学刊，2004，22(11)：2133-2134．

韩忠明，郭洪丽，常波，等．黄芩中黄芩苷微波提取工艺研究[J]．时珍国医国药，2011，22(12)：2840-2841．

李倩倩，王艳，罗旭，等．大豆低聚糖及其降血脂作用研究进展[J]．核农学报，2017，31(9)：1788-1793．

李景．大豆磷脂的功能特性及应用现状[J]．安徽农学通报，2017，23(22)：102-104．

国家药典委员会．中华人民共和国药典．一部[M]．北京：中国医药科技出版社，2015：301-302．

于良富，赵宇，鲍旭，等．黄芩回流提取工艺优选[J]．中国中医药现代远程教育，2016，14(17)：138-139．

丁婵，褚秀玲，苏建青，等．正交设计法优选黄芩苷的提取工艺研究[J]．中国畜牧杂志，2016，52(18)：72-75．

郭孝武．超声提取黄芩苷成分的实验研究[J]．中国现代应用药学杂志，1999，16(3)：18-20．

欧水平，王森，张海燕，等．黄芩苷溶解性能的测定与表征[J]．现代生物医学进展，2009，9(20)：3954-3957．

李俊生，吕佳佳，王兴慧，等．黄芩总黄酮及其单体的溶解性及体外经皮渗透性能研究[J]．中国中药杂志，2014，45(2)：200-207．

翟莹莹，于欢，龚千锋，等．猪心血炒酸枣仁的炮制工艺优选[J]．中国实验方剂学杂志，2017，23(24)：46-50．

臧琛，聂其霞，王国华，等．Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺[J]．中国实验方剂学杂志，2018，24(10)：15-20．

王赛君，伍振峰，杨明，等．中药提取新技术及其在国内的转化应用研究[J]．中国中药杂志，2014，39(8)：1360-1365．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Screening and Identification of Endophytic Fungi in Scutellariae Radix Transformed Baicalin to Baicalein and Oroxylin A

Medicine Property Variation in Different Proportional Combinations of Scutellariae Radix and Prepared Pinellia Tuber through Determination Leaching Quantity of Baicalin by HPLC

Optimization of Extraction Technology for Fufang Sihuang Suppository

Optimization of Flash-type Extraction Technology of Baicalin from by Response Surface Methodology

Characterization of Specific Chromatograms of Scutellariae Radix Before and After Being Processed by HPLC and HSGC

Related Author

Zong-min MA

Jia LIU

Xu-hong DUAN

Guo-qiang SUN

Lu-qi HUANG

Lin PEI

Li Ping

YANG Ying-yong

Related Institution

Hebei Key Laboratory of Turbiditytoxin Syndrome, Hebei Academy of Chinese Medical Sciences

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences

Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences

China Academy of Chinese Medical Sciences

College of Pharmacy，Gansu University of Chinese Medicine

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰