Plant Identification of Different Growth Forms of Justicia procumbens and Comparison of Their Chemical Components Content

Yun-feng YAO; Yi-yi XIONG; Wei-chen XIONG; Jiao-e GAN; Bo LIU; He-zhen WU; Yan-fang YANG

doi:10.13422/j.cnki.syfjx.20191411

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Plant Identification of Different Growth Forms of Justicia procumbens and Comparison of Their Chemical Components Content

Resource and Quality Evaluation | 更新时间：2021-02-09

- Plant Identification of Different Growth Forms of Justicia procumbens and Comparison of Their Chemical Components Content
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 21, Pages: 140-147(2019)
- 作者机构：
  
  1.湖北中医药大学　药学院，武汉　 430065；
  2.中药资源与中药化学湖北省重点实验室，武汉　 430061；
  3.老年病中药新产品湖北省协同创新中心，武汉　 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191411
  CLC： R284.1; R289; R22; R2-031
- Published：05 November 2019，
  
  Published Online：09 April 2019，
  
  Received：01 October 2018，
- 稿件说明：
扫描看全文
Yun-feng YAO, Yi-yi XIONG, Wei-chen XIONG, et al. Plant Identification of Different Growth Forms of Justicia procumbens and Comparison of Their Chemical Components Content. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):140-147(2019)
DOI：

Yun-feng YAO, Yi-yi XIONG, Wei-chen XIONG, et al. Plant Identification of Different Growth Forms of Justicia procumbens and Comparison of Their Chemical Components Content. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):140-147(2019) DOI： 10.13422/j.cnki.syfjx.20191411.

摘要

目的：

鉴别垂直生长(V类)和横向生长(L类)两类爵床并比较其地上部分及不同器官中化学成分含量的差异。

方法：

使用试剂盒提取两类爵床叶中的DNA，经聚合酶链式反应(PCR)扩增和双向测序得内部转录间隔区2(ITS2)序列，

psb

A-

trn

H序列；采用HPLC，测定两类爵床地上部分及花穗、叶和茎中爵床脂定B和金不换甲醚的含量；通过独立样本

检验和配对样本

检验，比较两类爵床地上部分及其不同器官中的爵床脂定B和金不换甲醚的含量差异。

结果：

将2个序列经过剪切后比对，V类和L类爵床的DNA序列完全一致；独立样本

检验结果表明，爵床脂定B的含量在两类爵床的地上部分中不存在显著差异，而金不换甲醚的含量为V类爵床显著高于L类(

0.05)；配对样本

检验结果表明，爵床脂定B和金不换甲醚的含量在同类爵床的花穗、叶和茎中均存在显著性差异(

0.05)，花穗和叶中的含量远大于茎；且金不换甲醚在V类爵床的花穗和叶中的含量要高于L类。

结论：

DNA条形码技术鉴别序列一致，表明两类不同生长形态的爵床为同种植物；不同生长形态的爵床植株中金不换甲醚的含量存在差异，V类爵床含量更高；同时，爵床茎中有效成分的含量极少，故在爵床的花穗和叶多的花、果期采收为佳。

Abstract

Objective:

To identify two different growth types vertical growth (V-class) and lateral growth (L-class)) of

Justicia procumbens

and compare the contents of chemical compounds between upper part and different organs.

Method:

DNA was extracted from the leaves of two growth forms of

procumbens

. Internal transcriptional spacer 2 (ITS2) and

psb

A-

trn

H sequences were obtained by polymerase chain reaction (PCR) amplification and bidirectional sequencing. High performance liquid chromatography (HPLC) was used to determine the contents of justicidin B (JB) and chinensinaphthol methyl ether (CME) from the above-ground parts of V-class and L-class

procumbens

and their inflorescence

leaves

and stems. Independent sample

-test and Paired-sample

-test were used to compare the contents of JB and CME from two different growth forms of

procumbens

and their different organs.

Result:

The DNA sequences after shearing were shown to be identical between the V-class and L-class samples. Independent sample

-test showed no difference on the content of JB between the above-ground parts of V-class and L-class samples

but the content of CME was higher in V-class

procumbens

than L-class (

0.05). Paired-sample

-test showed that the content of JB and CME were different in the inflorescence

leaves

and stems of

procumbens

(

0.05)

and the contents in inflorescence and leaves were much higher than those in stems. At the same time

we found the content of CME in inflorescence and leaves of V-class

procumbens

was higher than that in L-class.

Conclusion:

DNA barcoding and comparison of JB and CME content indicated that the two different growth forms of

procumbens

belong to the same species. The contents of CME were different in

procumbens

between different growth forms

and was higher in V-class. Meanwhile

due to the little content of JB and CME in stems

the best picking time of

procumbens

is the flowering and fruiting period with more inflorescence and leaves.

关键词

爵床垂直生长类横向生长类DNA条形码爵床脂定B金不换甲醚

Keywords

Justicia procumbensvertical growthlateral growthDNA barcodingjusticidin Bchinensinaphthol methyl ether

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