HPLC Determination of Four Flavonoids of Aurantii Fructus Immaturus in Niuhuang Qingwei Pills and Genetic Analysis of Medicinal Materials

Jing-jing LIU; Xiao-ru HU; Yi HE; Zhong DAI; Shuang-cheng MA

doi:10.13422/j.cnki.syfjx.20191517

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HPLC Determination of Four Flavonoids of Aurantii Fructus Immaturus in Niuhuang Qingwei Pills and Genetic Analysis of Medicinal Materials

Resource and Quality Evaluation | 更新时间：2021-02-09

- HPLC Determination of Four Flavonoids of Aurantii Fructus Immaturus in Niuhuang Qingwei Pills and Genetic Analysis of Medicinal Materials
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 155-159(2019)
- 作者机构：
  
  中国食品药品检定研究院，北京　 100050
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191517
  CLC： R284.1; R289; R22; R2-031
- Published：20 September 2019，
  
  Published Online：19 April 2019，
  
  Received：10 October 2018，
- 稿件说明：
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Jing-jing LIU, Xiao-ru HU, Yi HE, et al. HPLC Determination of Four Flavonoids of Aurantii Fructus Immaturus in Niuhuang Qingwei Pills and Genetic Analysis of Medicinal Materials. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):155-159(2019)
DOI：

Jing-jing LIU, Xiao-ru HU, Yi HE, et al. HPLC Determination of Four Flavonoids of Aurantii Fructus Immaturus in Niuhuang Qingwei Pills and Genetic Analysis of Medicinal Materials. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):155-159(2019) DOI： 10.13422/j.cnki.syfjx.20191517.

摘要

目的：

建立牛黄清胃丸中芸香橙皮苷、柚皮苷、橙皮苷、新橙皮苷含量测定方法，同时鉴别其中枳实来源。

方法：

采用高效液相色谱法，Waters CORTECS C

色谱柱(4.6 mm×50 mm，2.7 μm)，流动相乙腈-0.12%甲酸溶液梯度洗脱，检测波长283 nm，流速0.5 mL·min

－1

，柱温27 ℃。

结果：

12批次牛黄清胃丸样品分别呈现与酸橙或甜橙药材一致的黄酮类成分含量情况。芸香橙皮苷在5.47～2 735 ng线性良好(

＝0.999 6)，柚皮苷在7.25～3 625 ng线性良好(

＝0.999 5)，橙皮苷在8.41～4 205 ng线性良好(

＝0.999 4)，新橙皮苷在8.36～4 180 ng线性良好(

＝0.999 5)。12批次制剂中芸香橙皮苷质量分数在0.36～1.28 mg·g

－1

，柚皮苷在2.66～4.87 mg·g

－1

，橙皮苷在1.02～11.07 mg·g

－1

，新橙皮苷在3.58～6.41 mg·g

－1

，总黄酮在7.98～13.34 mg·g

－1

。芸香橙皮苷、柚皮苷、橙皮苷、新橙皮苷平均加样回收率分别为101.3%，98.0%，95.9%，96.0%，RSD为2.9%，1.8%，0.8%，1.1%。

结论：

该方法简便、准确、可靠，可同时鉴别牛黄清胃丸中枳实来源，并测定其中4种黄酮类主要有效成分的含量，为2种牛黄清胃丸质量控制和成分对比提供了基础，检测指标更全面，可为评价牛黄清胃丸质量提供依据。

Abstract

Objective:

To establish an high performance liquid chromatography (HPLC) method for the simultaneous determination of four constituents in Niuhuang Qingwei pill (narirutin

naringin

hesperidin

and neohesperidin)

and identify the source of Fructus Aurantii Immaturus.

Method:

The analysis was performed on a Waters CORTECS C

column (4.6 mm×50 mm

2.7 μm)

with acetonitrile-0.12% formic acid solution as the mobile phase for gradient elution. The flow rate was 0.5 mL·min

-1

the detection wavelength was set at 283 nm

and the column temperature was 27 ℃.

Result:

12 batches of Niuhuang Qingwei pills showed the different content of flavonoids as

Citrus aurantium

and

sinensis

. Narirutin

naringin

hesperidin and neohesperidin showed good linear relationships within the ranges of 5.47-2 735 ng (

=0.999 6)

7.25-3 625 ng (

=0.999 5)

8.41-4 205 ng (

=0.999 4) and 8.36-4 180 ng (

=0.999 5)

and their average recoveries were 101.3% (

RSD 2.9%)

98.0% (

RSD 1.8%)

95.9% (

RSD 0.8%) and 96.0% (

RSD 1.1%)

respectively. The contents of narirutin

naringin

hesperidin

neohesperidin and total flavonoids were 0.36-1.28

2.66-4.87

1.02-11.07

3.58-6.41

and 7.98-13.34 mg·g

-1

respectively.

Conclusion:

The developed method was simple

accurate and reliable

which can be used to identify the source of Aurantii Immaturus Fructus and simultaneously determine the content of four flavonoids in Niuhuang Qingwei pills. It could provide basic research for quality control and composition comparison of 2 kinds of Niuhuang Qingwei pills

showing more comprehensive indicators and reference value for the quality standard improvement of Niuhuang Qingwei pill.

关键词

牛黄清胃丸酸橙甜橙芸香橙皮苷柚皮苷橙皮苷新橙皮苷

Keywords

Niuhuang Qingwei pillCitrus aurantiumCitrus sinensisnarirutinnaringinhesperidinneohesperidin

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