Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice

Li-na MA; Jian LI; Si-di LI; Bao-qiang DAI; Guang-ping ZHANG; Hong-ping HOU; Ping SU; Feng-yu WANG; Zu-guang YE

doi:10.13422/j.cnki.syfjx.20191722

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Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice

Pharmacology | 更新时间：2021-02-09

- Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 22, Pages: 34-41(2019)
- 作者机构：
  
  中国中医科学院　中药研究所，博士后科研流动站，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191722
  CLC： R22; R242; R2-031; R285.5
- Published：20 November 2019，
  
  Published Online：22 May 2019，
  
  Received：06 January 2019，
- 稿件说明：
扫描看全文
Li-na MA, Jian LI, Si-di LI, et al. Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(22):34-41(2019)
DOI：

Li-na MA, Jian LI, Si-di LI, et al. Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(22):34-41(2019) DOI： 10.13422/j.cnki.syfjx.20191722.

摘要

目的：

探究常山碱盐(dichroa alkali salt

DAS)诱导呕吐的可能机制。

方法：

采用小鼠异食癖模型，分别观察3种不同机制的镇吐药，多巴胺受体拮抗剂甲氧氯普胺，5-羟色胺3(5-hydroxytryptamine 3，5-HT

)受体拮抗剂昂丹司琼，神经激肽-1受体拮抗剂阿瑞匹坦，对DAS所致小鼠体质量、正常饲料摄食量、高岭土摄食量及腹泻和死亡情况的影响，明确DAS可能的致吐途径；然后采用酶联免疫法针对性的检测DAS干预后不同时间点小鼠回肠和延髓中5-HT和P物质(substance P，SP)的表达情况，以确认DAS是否可影响这2种神经递质的变化。

结果：

配伍昂丹司琼和阿瑞匹坦后，连续给药第4天和药后第1天均可显著改善DAS诱导的小鼠摄食量降低现象(

0.01)，自连续给药第3天起均可显著改善DAS诱导的小鼠体质量降低现象(

0.01)，降低DAS诱导的小鼠腹泻率和死亡率，同时可有效拮抗DAS诱导的小鼠异食行为，但是甲氧氯普胺对上述指标则无明显改善作用；进一步检测呕吐相关神经递质，发现与空白组比较，给予DAS后4，12 h回肠和延髓中5-HT含量显著增加，48 h时回肠和延髓中SP含量显著升高。

结论：

小鼠异食模型可有效表征DAS诱导的呕吐现象，其诱导的呕吐行为可能与回肠和延髓中5-HT和SP含量升高有关，配伍昂丹司琼和阿瑞匹坦可有效拮抗DAS诱导的小鼠异食现象。

Abstract

Objective:

To study the possible mechanism of dichroa alkali salt (DAS) in inducing vomiting.

Method:

Mice pica model was used to observe the antagonistic effect of the three different kinds of antiemetic drugs [dopamine receptor antagonist metoclopramide

5-hydroxytryptamine 3 (5-HT

) receptor antagonist ondansetron and neurokinin-1 receptor antagonist aprepitant]

on body mass

food intake

kaolin consumption

diarrhea and death induced by DAS to preliminarily clarify the possible pathogenic pathway of DAS. Then

the expression of 5-HT and substance P(SP) in ileum and medulla of mice induced by DAS alone at different time points was detected by enzyme-linked immunosorbent assay to confirm whether DAS could affect the changes of these two neurotransmitters.

Result:

After treatment with ondansetron and aprepitant

DAS-induced reduction in food intake of mice was significantly improved on the 4

day after continuous administration and on the 1

day after drug administration (

0.01)

since the 3

day after administration

DAS-induced body mass loss of mice was significantly improved (

0.01)

and DAS-induced diarrhea and mortality in mice were significantly reduced

while DAS-induced pica in mice was effectively antagonized. However

metoclopramide did not significantly improve the above indicators. Further detection of vomiting-related neurotransmitters found that compared with the blank group

the expressions of 5-HT at 4 h

12 h and SP at 48 h after treatment with DAS were significantly increased in ileum and medulla of mice.

Conclusion:

The mice pica model can be used to effectively characterize DAS-induced vomiting. DAS-induced pica in mice may be associated with the increase of 5-HT and SP in ileum and medulla. Ondansetron and aprepitant can effectively antagonize DAS-induced pica in mice.

关键词

常山碱盐呕吐作用小鼠异食癖配伍镇吐药

Keywords

dichroa alkali saltvomitingpica in micecompatibilityantiemetic drug

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