Suspension Culture and Alkaloid Content of Pinelliae Rhizoma Tuber Under Stimulation of Salicylic Acid

Xing-kui TAO; Ming-mei HUANG; Jian-ping XUE; Ai-min ZHANG; Xing-tao ZHANG; Hai-chao WANG

doi:10.13422/j.cnki.syfjx.20191814

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Suspension Culture and Alkaloid Content of Pinelliae Rhizoma Tuber Under Stimulation of Salicylic Acid

Resource and Quality Evaluation | 更新时间：2021-02-09

- Suspension Culture and Alkaloid Content of Pinelliae Rhizoma Tuber Under Stimulation of Salicylic Acid
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 18, Pages: 139-144(2019)
- 作者机构：
  
  1.淮北师范大学　生命科学学院，资源植物生物学安徽省重点实验室，安徽　淮北　 235000；
  2.宿州学院　生物与食品工程学院，宿州　 234000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191814
  CLC： R284.2; R289; R22; R2-031
- Published：20 September 2019，
  
  Published Online：02 July 2019，
  
  Received：07 May 2019，
- 稿件说明：
扫描看全文
Xing-kui TAO, Ming-mei HUANG, Jian-ping XUE, et al. Suspension Culture and Alkaloid Content of Pinelliae Rhizoma Tuber Under Stimulation of Salicylic Acid. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):139-144(2019)
DOI：

Xing-kui TAO, Ming-mei HUANG, Jian-ping XUE, et al. Suspension Culture and Alkaloid Content of Pinelliae Rhizoma Tuber Under Stimulation of Salicylic Acid. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(18):139-144(2019) DOI： 10.13422/j.cnki.syfjx.20191814.

摘要

目的：

研究水杨酸刺激下半夏试管块茎悬浮培养及其体内生物碱含量的变化。

方法：

以半夏悬浮试管小块茎为材料，对其处理不同浓度的外源水杨酸刺激，分析块茎的生长情况，利用高效液相色谱法(HPLC)测定块茎中生物碱的含量。检测采用Agilent C

色谱柱(4.6 mm×250 mm，5μm)，流动相乙腈-水(4∶96)，柱温35 ℃，肌苷检测波长250 nm，鸟苷检测波长260 nm，流速1.0 mL·min

－1

，进样量10 μL。

结果：

外源添加不同浓度的水杨酸，处理不同的时间，半夏块茎的鲜重在第25天，水杨酸150 μmol·L

－1

时达到最大7.483 8 g。同时积累了一定量的生物碱，鸟苷在0.03～0.45 μg(

＝0.999 6)线性关系良好，当水杨酸浓度为50 μmol·L

－1

时，培养10 d，半夏块茎中鸟苷的质量分数达到最大值1.353 3 mg·g

－1

；肌苷在0.003～0.045 μg(

＝0.999 5)线性关系良好，在水杨酸浓度为200 μmol·L

－1

时，培养30 d，半夏块茎中肌苷的质量分数达到最大值0.149 8 mg·g

－1

。

结论：

该研究结果为半夏试管块茎的组培快繁和生物碱的调控研究提供参考，对半夏产业的发展具有十分重要的意义。

Abstract

Objective:

To study the suspension culture of tuber and its alkaloid content based on the stimulation of salicylic acid.

Method:

The tubers of Pinelliae Rhizoma in suspension tube were treated with different concentrations of exogenous salicylic acid to analyze the growth status. The content of alkaloids in tuber was detected by HPLC. Test conditions: chromatographic column for Agilent Eclipse plus C

column (4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile water(4∶96)

the column temperature was maintained at 35 ℃

detection wavelength for inosine 250 nm

guanosine 260 nm

volume flow rate 1.0 mL·min

-1

Result:

The results showed that the exogenous salicylic acid had a certain effect on the growth of suspension tuber of Pinelliae Rhizoma. When the salicylic acid concentration was 150 μmol·L

-1

the culture lasted for 25 days and the fresh weight reached the maximum value of 7.483 8 g. It also accumulates a certain amount of alkaloids. The linear range of guanosine was 0.03-0.45 μg (

=0.999 6). After 10-days cultivatation in the salicylic acid concentration of 50 μmol·L

-1

guanosine content of Pinelliae Rhizoma tubers reached a maximum of 1.353 3 mg·g

-1

. The linear range of inosine 0.003-0.045 μg (

=0.999 5). When the salicylic acid concentration was 200 μmol·L

-1

cultured for 30 days

the content of inosine in Pinelliae Rhizoma tubers reached the maximum value of 0.149 8 mg·g

-1

Conclusion:

The results of this experiment provide a reference for the study of tissue culture and rapid propagation of Pinelliae Rhizoma tubers and regulation of alkaloids

which are of great significance for the development of Pinelliae Rhizoma industry.

关键词

半夏试管块茎外源性刺激水杨酸生物碱

Keywords

Pinelliae Rhizomatuberexogenous stimulussalicylic acidalkaloid

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