Effect of Pinelliae Rhizoma Praeparatum Cum Alumine Polysaccharides on MUC5AC mRNA in Lung Tissues of Allergic Asthma Model Rats

Cong HUANG; Wei PENG; Da-neng WEI; Zhan-qiong ZHONG; Mao-ting MOU; Shu-jun WEI; Yong-xiang GAO; Chun-jie WU

doi:10.13422/j.cnki.syfjx.20192103

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Effect of Pinelliae Rhizoma Praeparatum Cum Alumine Polysaccharides on MUC5AC mRNA in Lung Tissues of Allergic Asthma Model Rats

Pharmacology | 更新时间：2021-02-09

- Effect of Pinelliae Rhizoma Praeparatum Cum Alumine Polysaccharides on MUC5AC mRNA in Lung Tissues of Allergic Asthma Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 22, Pages: 15-21(2019)
- 作者机构：
  
  成都中医药大学，成都　 611137
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192103
  CLC： R2-0; R22; R285.5
- Published：20 November 2019，
  
  Published Online：18 July 2019，
  
  Received：27 March 2019，
- 稿件说明：
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Cong HUANG, Wei PENG, Da-neng WEI, et al. Effect of Pinelliae Rhizoma Praeparatum Cum Alumine Polysaccharides on MUC5AC mRNA in Lung Tissues of Allergic Asthma Model Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(22):15-21(2019)
DOI：

Cong HUANG, Wei PENG, Da-neng WEI, et al. Effect of Pinelliae Rhizoma Praeparatum Cum Alumine Polysaccharides on MUC5AC mRNA in Lung Tissues of Allergic Asthma Model Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(22):15-21(2019) DOI： 10.13422/j.cnki.syfjx.20192103.

摘要

目的：

通过观察清半夏多糖对过敏性哮喘模型大鼠气道黏液分泌的作用，研究清半夏多糖为原药材发挥“燥湿化痰”功效“大分子”成分的物质作用基础。

方法：

SPF级Wistar大鼠60只，除正常组外，鸡卵清蛋白(OVA)与铝镁佐剂腹腔注射诱导致敏，OVA溶液雾化激发，建立过敏性哮喘大鼠模型。造模成功后大鼠随机平均分成5组，为过敏性哮喘模型组，阳性药组(孟鲁司特钠，5 mg·kg

－1

)，清半夏多糖高、中、低剂量组(400，200，100 mg·kg

－1

)。酶联免疫吸附测定(ELISA)检测血清、支气管肺泡灌洗液(BALF)上清液中白细胞介素-4(IL-4)，

-干扰素(IFN-

)含量，BALF沉渣进行嗜酸性粒细胞(EOS)的计数检测；苏木素-伊红(HE)染色观察肺组织病理改变，实时荧光定量聚合酶链式反应(Real-time PCR)检测肺组织黏蛋白5AC(MUC5AC)mRNA的表达。

结果：

与正常组比较，过敏性哮喘模型组大鼠血清IL-4含量显著升高(

0.01)，IFN-

含量显著降低(

0.01)，BALF中EOS计数显著升高(

0.01)；与过敏性哮喘模型组比较，孟鲁司特钠组、清半夏多糖高、中、低剂量组均能显著降低血清中IL-4的含量(

0.01)，清半夏多糖高、中剂量组能显著降低BALF中IL-4的含量(

0.05，

0.01)，清半夏多糖中剂量组能显著增高血清中IFN-

的含量(

0.05)，孟鲁司特钠组、清半夏多糖各剂量组均能显著降低BALF中的EOS计数(

0.01)。HE染色结果显示，过敏性哮喘模型组大鼠肺组织出现明显病理改变，孟鲁司特钠组、清半夏多糖各组具有缓解作用。与正常组比较，过敏性哮喘模型组大鼠肺组织的MUC5AC mRNA表达显著升高(

0.01)；与过敏性哮喘模型组比较，孟鲁司特钠组、清半夏多糖高、中、低剂量组MUC5AC mRNA均显著降低(

0.01)。

结论：

清半夏的“大分子”成分清半夏多糖，可能是原药材清半夏发挥“燥湿化痰”功效的物质作用基础。

Abstract

Objective:

To observe the effect of Pinelliae Rhizoma Praeparatum Cum Alumine polysaccharides(PRPCAP)on airway mucus secretion in rats with allergic asthma

in order to study the material basis of the " macromolecule" component of the polysaccharides as the original medicinal materials.

Method:

The 60 SPF-grade Wistar rats were induced by intraperitoneal injection of chicken ovalbumin (OVA) and aluminum-magnesium adjuvant

except for the normal control group. The OVA solution was aerosolized to establish a rat model of allergic asthma. After successful modeling

the rats were randomly divided into 5 groups

namely allergic asthma model group

positive drug group (montalurast sodium

5 mg·kg

-1

)

high-dose PRPCAP group (400 mg·kg

-1

)

middle-dose PRPCAP group (200 mg·kg

-1

) and low-dose PRPCAP group (100 mg·kg

-1

). The contents of interleukin-4 (IL-4) and interferon-

(IFN-

) in serum and bronchoalveolar lavage fluid (BALF) supernatant were determined by enzyme-linked immunosorbent assay (ELISA)

and the count of eosinophils (EOS) was detected by BALF sediment. The histopathological changes were observed by hematoxylin-eosin (HE) staining in lung tissue. The mRNA expression of mucin 5AC (MUC5AC) was detected by Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR).

Result:

Compared with the normal control group

serum IL-4 level in the allergic asthma model group was significantly increased (

0.01)

IFN-

level was significantly decreased (

0.01)

and EOS counts in BALF were significantly increased (

0.01). Compared with the allergic asthma model group

the positive drug group

and high

middle

and low-dose PRPCAP groups could significantly reduce the serum IL-4 content (

0.01)

while high and middle-dose PRPCAP groups could significantly reduce the content of IL-4 in BALF (

0.05

0.01)

the middle-dose PRPCAP group could significantly increase the content of IFN-

in serum (

0.05)

EOS counts in BALF were significantly lower in the positive drug group and all of PRPCAP groups (

0.01). HE staining showed the pathological changes in the lung tissue of the allergic asthma model group and a mitigating effect in the positive drug group and all of PRPCAP groups. Compared with the normal control group

the expression of MUC5AC mRNA in the lung tissue of rats with allergic asthma was significantly increased (

0.01). Compared with the model group of allergic asthma rats

the positive drug group and all of PRPCAP groups showed significant decrease in MUC5AC mRNA (

0.01).

Conclusion:

The " macromolecule" component of polysaccharides in the Pinelliae Rhizoma Praeparatum Cum Alumine may be the material basis for the efficacy of eliminating dampness and eliminating phlegm.

关键词

清半夏多糖过敏性哮喘模型大鼠黏蛋白5AC(MUC5AC)

Keywords

Pinelliae Rhizoma Praeparatum Cum Alumine polysaccharidesallergic asthma model ratmucin 5AC (MUC5AC)

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