Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β<sub>1</sub> mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats

Qing-xue WANG; Qi-qi ZHANG; Chun-yan LIU; Shi-yan LUO; Yi-hui CHAI

doi:10.13422/j.cnki.syfjx.20192105

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Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats

Pharmacology | 更新时间：2021-02-09

- Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 24, Pages: 43-47(2019)
- 作者机构：
  
  贵州中医药大学，贵阳　 550025
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192105
  CLC： R2-0; R22; R285.5; R289
- Published：20 December 2019，
  
  Published Online：18 July 2019，
  
  Received：01 April 2019，
- 稿件说明：
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Qing-xue WANG, Qi-qi ZHANG, Chun-yan LIU, et al. Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):43-47(2019)
DOI：

Qing-xue WANG, Qi-qi ZHANG, Chun-yan LIU, et al. Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):43-47(2019) DOI： 10.13422/j.cnki.syfjx.20192105.

摘要

目的：

探讨中药解酒方葛根散对酒精性勃起功能障碍(ED)大鼠阴茎平滑肌组织结构一氧化氮合酶(NOS)活力及

-平滑肌肌动蛋白(

-SMA)，缝隙连接蛋白基因43(Cx43)，转化生长因子-

(TGF-

) mRNA和蛋白表达的影响，为临床应用葛根散治疗酒精性ED提供实验依据。

方法：

将SD大鼠随机分为5组，分别为正常组、模型组、葛根散低、中、高剂量组(5，10，20 g·kg

－1

)；除正常组外，其他各组按照15 mL·kg

－1

·d

－1

给予乙醇干预30 min后给药。比色法检测酒精性ED大鼠阴茎平滑肌组织NOS活力；实时荧光定量聚合酶链式反应法(Real-time PCR)，蛋白免疫印迹法(Western blot)检测酒精性ED大鼠阴茎平滑肌组织

-SMA，Cx43，TGF-

mRNA及蛋白表达的情况。

结果：

与正常组比较，模型组大鼠阴茎平滑肌组织NOS活力，

-SMA，Cx43 mRNA及蛋白表达量显著下降(

0.05)，TGF-

mRNA及蛋白表达量显著上升(

0.05)。与模型组比较，葛根散低、中、高剂量组均可显著上调酒精性ED大鼠阴茎组织结构NOS活力及Cx43 mRNA及蛋白表达量(

0.05)，同时可显著下调TGF-

mRNA及蛋白表达量(

0.05)，葛根散中、高剂量组均可显著上调酒精性ED大鼠阴茎组织

-SMA mRNA及蛋白表达量(

0.05)。

结论：

葛根散对酒精性ED大鼠阴茎平滑肌组织结构有明显的保护作用，其具体机制可能与调节NOS活力及

-SMA，Cx43，TGF-

mRNA及蛋白表达量有关。

Abstract

Objective:

To investigate the activity of nitric oxide synthase (NOS) and

-smooth actin (

-SMA) in rat penile smooth muscle tissue of rats with alcoholic erectile dysfunction (ED). The effects of protein gene 43 (connexin43

Cx43) and transforming growth factor

(TGF-

) mRNA and protein expressions provide an experimental basis for the clinical application of Gegensan in the treatment of alcoholic ED.

Method:

SD rats were randomly divided into five groups: normal group

model group

and low

medium

high-dose Gegensan groups (5

20 g·kg

-1

). Except the normal group

the other groups were administered with drugs after alcohol intervention for 30 min at 15 mL· kg

-1

·d

-1

. Colorimetric assay was used to detect NOS activity in the penile smooth muscle tissue of alcoholic ED rats. Quantitative real-time fluorescence polymerase chain reaction(Real-time PCR) and Western blot were used to detect

-SMA

Cx43

TGF-

mRNA and protein expressions in smooth muscle tissue of alcoholic ED rats.

Result:

Compared with the normal group

the expressions of NOS

-SMA and Cx43 mRNA and protein in the penile smooth muscle of the model group decreased significantly (

0.05)

while the expressions of TGF-

mRNA and protein increased significantly (

0.05). Compared with the model group

low/medium/high-dose Gegensan groups could significantly up-regulate the NOS activity and Cx43 mRNA and protein expressions in the penile tissue of alcoholic ED rats (P

0.05)

while significantly down-regulate TGF-

mRNA expression

and

-SMA mRNA and protein expressions in the penis tissue of rats with alcoholic ED were significantly up-regulated (

0.05).

Conclusion:

Gegensan has an obvious protective effect on the structure of penile smooth muscle of alcoholic ED rats. The specific mechanism may be related to the regulation of NOS activity and a-SMA

Cx43 and TGF-

mRNA and protein expressions.

关键词

葛根散乙醇勃起功能障碍一氧化氮合酶α-平滑肌肌动蛋白缝隙连接蛋白基因43转化生长因子-β1

Keywords

Gegensanalcoholerectile dysfunctionnitric oxide synthaseα-smooth actingap junction protein gene 43transforming growth factor-β1

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Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β1 mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats

DOI：10.13422/j.cnki.syfjx.20192105

摘要

Abstract

关键词

Keywords

references

Effect of Gegensan on Expressions of NOS, α-SMA, Cx43, TGF-β₁ mRNA and Protein in Penile Smooth Muscle Tissue of Alcoholic ED Rats