Gastrointestinal Regulation of Red Yeast Rice on Mice with Syndrome of Food Retention Due to Spleen Deficiency Before and After Fermentation

Jia LUO; Qiang SUN; Zu-bing MA; Zu-xin HE; Xiao-fang LI

doi:10.13422/j.cnki.syfjx.20192252

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Gastrointestinal Regulation of Red Yeast Rice on Mice with Syndrome of Food Retention Due to Spleen Deficiency Before and After Fermentation

Pharmacy and Processing | 更新时间：2021-02-09

- Gastrointestinal Regulation of Red Yeast Rice on Mice with Syndrome of Food Retention Due to Spleen Deficiency Before and After Fermentation
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 22, Pages: 108-114(2019)
- 作者机构：
  
  1.成都中医药大学，成都　 611137；
  2.四川得恩德药业有限公司，四川　绵阳　 612000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192252
  CLC： R22; R24; R28; C37; TS193; R943.1; R378.2; TQ92
- Published：20 November 2019，
  
  Published Online：02 August 2019，
  
  Received：19 June 2019，
- 稿件说明：
扫描看全文
Jia LUO, Qiang SUN, Zu-bing MA, et al. Gastrointestinal Regulation of Red Yeast Rice on Mice with Syndrome of Food Retention Due to Spleen Deficiency Before and After Fermentation. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(22):108-114(2019)
DOI：

Jia LUO, Qiang SUN, Zu-bing MA, et al. Gastrointestinal Regulation of Red Yeast Rice on Mice with Syndrome of Food Retention Due to Spleen Deficiency Before and After Fermentation. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(22):108-114(2019) DOI： 10.13422/j.cnki.syfjx.20192252.

摘要

目的：

研究红曲发酵炮制对脾虚食积证小鼠的胃肠调节作用及其作用机制，为曲类中药的健脾消食作用机制探索新思路。

方法：

采用随机分组法将50只昆明小鼠分为5组，包括空白组、模型组、红曲未发酵组(1.17 g·kg

－1

·d

－1

)，红曲发酵组(1.17 g·kg

－1

·d

－1

)，多潘立酮组(3.9 mg·kg

－1

·d

－1

)，每组10只，连续灌胃给药10 d。对比红曲发酵炮制前后对脾虚食积证小鼠行为学、体质量、进食量、胃内残留率和小肠推进率的影响。采用酶联免疫吸附测定法(ELISA)检测红曲对脾虚食积证小鼠血清中胃动素(MTL)，胃泌素(GAS)，5-羟色胺(5-HT)和血管活性肠肽(VIP)水平的影响；采用苏木素-伊红(HE)染色法研究红曲对脾虚食积证小鼠胃肠组织形态学的影响；基于细菌16S rRNA基因序列的V3-V4区，通过高通量测序平台Illumina MiSeq研究红曲对脾虚食积证小鼠肠道菌群结构的调节作用。

结果：

与模型组比较，红曲发酵后可恢复脾虚食积证小鼠的体质量和进食量，显著降低胃内残留率(

0.01)和显著升高小肠推进率(

0.01)。红曲可增加脾虚食积证小鼠胃肠激素MTL和5-HT的水平，降低VIP水平；红曲能改善脾虚食积证小鼠胃肠组织形态，改善脾虚食积证小鼠胃黏膜的轻微充血和局部腺体排列不整齐，显著升高脾虚食积证小鼠小肠绒毛长度、增加小肠黏膜厚度；红曲可以恢复脾虚食积证小鼠肠道菌群结构，增加有益菌双歧杆菌属和乳酸杆菌属的相对丰度，降低有害菌大肠埃希菌-志贺菌的相对丰度。

结论：

红曲发酵后能调节脾虚食积证小鼠的胃肠功能，其机制可能与调节胃肠激素水平、改善胃肠组织形态、恢复肠道菌群结构有关。

Abstract

Objective:

To investigate gastrointestinal regulation of red yeast rice on spleen deficient dyspepsia mice

and provide reference for clarifying mechanism of invigorating spleen to promote digestion of fermented traditional Chinese medicine.

Method:

Mice were randomly divided into 5 groups

including the blank group

the model group

the unfermented red yeast rice treatment group (1.17 g·kg

-1

·d

-1

)

the fermented red yeast rice treatment group (1.17 g·kg

-1

·d

-1

)

the domperidone group (3.9 mg·kg

-1

·d

-1

)

ten mice in each group were continuous intragastric administration for 10 days. The effects of red yeast rice before and after fermentation on the general behavior

body weight

food intake and gastrointestinal function of spleen deficient dyspepsia mice were compared. The effects of red yeast rice on the gastrointestinal hormone [motilin (MTL)

gastrin (GAS)

5-hydroxytryptamine (5-HT)

vasoactive intestinal peptide (VIP)]

levels in serum of spleen deficient dyspepsia mice were determined by enzyme-linked immunosorption assay (ELISA). The regulation function of red yeast rice on the pathological changes of gastrointestinal tissue of spleen deficient dyspepsia mice was investigated by hematoxylin-eosin (HE) staining. The effect of red yeast rice on gut microbiota of spleen deficient dyspepsia mice was investigated

the primers were designed on bacterial 16S rRNA V3-V4 region sequences and Illumina Miseq platform was used for high-throughput sequencing.

Result:

Compared with the model group

fermented red yeast rice could recover the body weight and food intake

reduce gastric residual rate (

0.01) and enhance the small intestinal propulsive rate (

0.01) of spleen deficient dyspepsia mice. Red yeast rice could increase the gastrointestinal hormone levels of MTL and 5-HT

while reduce the VIP level of spleen deficient dyspepsia mice. Red yeast rice could restore the light congestion and local structural disorder glands of gastric mucosa of spleen deficient dyspepsia mice. Red yeast rice could restore the pathological changes of intestinal tissue of spleen deficient dyspepsia mice by means of increasing the length of intestinal villus and the thickness of mucosa. Red yeast rice could restore the structure of the gut microbiota of spleen deficient dyspepsia mice by means of reducing the relative abundance of pathogenic microorganisms such as

Escherichia

Shigella

while increasing the relative abundance of the beneficial bacteria such as

Lactobacillus

and

Bifidobacterium

Conclusion:

Fermented red yeast rice can enhance the gastrointestinal function of spleen deficient dyspepsia mice

the mechanism may be related to regulating gastrointestinal hormone level

improving histopathology of gastrointestinal tissue and restoring intestinal flora structure.

关键词

红曲发酵炮制脾虚食积证胃肠调节作用胃肠激素胃肠组织形态肠道菌群

Keywords

red yeast ricefermentationsyndrome of food retentiondue to spleen deficiencygastrointestinal regulationgastrointestinal hormonesgastrointestinal tissue morphologygut microbiota

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Related Institution

Graduate School， Changchun University of Chinese Medicine

Graduate School， Beijing University of Chinese Medicine

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Key Laboratory of Modern Preparation of Traditional Chinese Medicine， Ministry of Education， Jiangxi University of Chinese Medicine

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