Effect of Shaoyao Gancaotang and Its Disassembled Prescriptions on Expressions of TRPV3, TRPV4 in Myocardium and Hypothalamus of Rats with Ultra-high Temperature

Feng-yun YAO; Lin WANG; Chun-hua LIU; Fei-yun WANG; Chao LIU; Jie-lin JIANG; Bing-zhi WANG

doi:10.13422/j.cnki.syfjx.20192301

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Effect of Shaoyao Gancaotang and Its Disassembled Prescriptions on Expressions of TRPV3, TRPV4 in Myocardium and Hypothalamus of Rats with Ultra-high Temperature

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Shaoyao Gancaotang and Its Disassembled Prescriptions on Expressions of TRPV3, TRPV4 in Myocardium and Hypothalamus of Rats with Ultra-high Temperature
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 24, Pages: 9-14(2019)
- 作者机构：
  
  江西中医药大学　中医学院，南昌　 330004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192301
  CLC： R2-0; R22; R285.5; R289
- Received：24 May 2019，
  
  Published Online：20 August 2019，
  
  Published：20 December 2019
- 稿件说明：
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Feng-yun YAO, Lin WANG, Chun-hua LIU, et al. Effect of Shaoyao Gancaotang and Its Disassembled Prescriptions on Expressions of TRPV3, TRPV4 in Myocardium and Hypothalamus of Rats with Ultra-high Temperature[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(24): 9-14.
DOI：

Feng-yun YAO, Lin WANG, Chun-hua LIU, et al. Effect of Shaoyao Gancaotang and Its Disassembled Prescriptions on Expressions of TRPV3, TRPV4 in Myocardium and Hypothalamus of Rats with Ultra-high Temperature[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(24): 9-14. DOI： 10.13422/j.cnki.syfjx.20192301.

摘要

目的：

通过观察芍药甘草汤及其拆方对超高温环境大鼠心肌、下丘脑瞬时感受器电位香草酸受体3(TRPV3)和瞬时感受器电位香草酸受体4(TRPV4)表达的影响，探讨芍药甘草汤及其拆方抗高温的机制。

方法：

SPF级SD雄性大鼠75只，适应饲养1周后，按体质量随机分为5组，分别为常温正常组，高温正常组，芍药甘草汤组(2.16 g·kg

－1

)，单味白芍组(1.08 g·kg

－1

)和单味甘草组(1.08 g·kg

－1

)，除常温正常组外，均饲养于人工气候箱，各给药组按相应剂量连续给药13 d，常温正常组和高温正常组均给予同体积的纯净水；通过蛋白免疫印迹法(Western blot)和实时荧光定量聚合酶链式反应(Real-time PCR)测定各组心肌、下丘脑TRPV3，TRPV4蛋白和mRNA的表达情况。

结果：

与常温正常组比较，高温正常组大鼠心肌TRPV3蛋白表达显著上升(

0.01)；心肌TRPV4 mRNA表达明显下降(

0.05)；下丘脑TRPV3蛋白表达显著上升(

0.01)；下丘脑TRPV3 mRNA表达明显下降(

0.05)。与高温正常组比较，单味白芍组大鼠心肌TRPV3，TRPV4蛋白表达显著下降(

0.01)；芍药甘草汤组与单味甘草组大鼠心肌TRPV4蛋白表达明显下降(

0.05)；单味白芍组大鼠心肌TRPV4 mRNA表达明显上升(

0.05)。

结论：

大鼠心肌、下丘脑TRPV3，TRPV4通道在高温环境下被激活，芍药甘草汤及其拆方抗高温机制与调节大鼠心肌、下丘脑TRPV3，TRPV4通道蛋白及mRNA表达有关。

Abstract

Objective:

To observe the effect of Shaoyao Gancaotang and its disassembled prescriptions on the expressions of transient receptor potential vanilloid 3 (TRPV3) and transient receptor potential vanilloid 4 (TRPV4) in myocardium and hypothalamus of rats in the ultra-high temperature environment

in order to explore the mechanism of Shaoyao Gancaotang and its disassembled prescriptions against the high temperature.

Method:

Seventy five male SPF-grade SD rats were randomly divided into 5 groups according to their body weight

namely normal control group

high temperature normal control group

Shaoyao Gancaotang group (2.16 g·kg

-1

)

single Paeoniae Radix Alba(PRA) group (1.08 g·kg

-1

) and single Glycyrrhizae Radix et Rhizoma(GRR) group (1.08 g·kg

-1

). Except normal control group

the remaining groups were fed in artificial climate boxes for 13 days. The normal control group and the high temperature normal control group were given the same volume of pure water. Western blot and quantitative real-time fluorescence polymerase chain reaction(Real-time PCR) were used to determine expressions of TRPV3

TRPV4 protein and mRNA in myocardium and hypothalamus of each group.

Result:

Compared with the normothermic control group

the expressions of TRPV3 protein

TRPV4 mRNA

TRPV3 protein and TRPV3 mRNA in the myocardium of the hyperthermic control group were significantly higher than those of the normal control group (

0.05)

and the expression of TRPV3 protein in the hypothalamus was significantly higher than that of the normal control group (

0.05). Compared with the normal control group

the expressions of TRPV3

TRPV4 proteins in myocardium of rats in PRA group were significantly lower than those in the control group

and the expression of TRPV4 protein in myocardium of rats in Shaoyao Gancaotang group and GRR group was significantly lower than that in control group. The expression of TRPV4 mRNA in myocardium of rats in PRA group was significantly increased (

0.05).

Conclusion:

TRPV3

TRPV4 channels in rat myocardium and hypothalamus were activated in the high temperature environment. The anti-hyperthermic mechanism of Shaoyao Gancaotang and its disassembled prescriptions was related to the regulation of TRPV3

TRPV4 channel protein and mRNA expressions in rat myocardium and hypothalamus.

关键词

Keywords

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