Quality Relevance of Standard Decoction and Different Drying Methods Products for Gentianae Macrophyllae Radix Granules Based on HPLC Fingerprint

Chao GUO; Shu-zhen JIAO; Chao XU; Cai-qin HAO; Xiao-hong LENG

doi:10.13422/j.cnki.syfjx.20192314

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Quality Relevance of Standard Decoction and Different Drying Methods Products for Gentianae Macrophyllae Radix Granules Based on HPLC Fingerprint

Resource and Quality Evaluation | 更新时间：2021-02-09

- Quality Relevance of Standard Decoction and Different Drying Methods Products for Gentianae Macrophyllae Radix Granules Based on HPLC Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 24, Pages: 118-123(2019)
- 作者机构：
  
  1.宁夏职业技术学院，银川　 750001；
  2.宁夏大学　农学院，银川　 750021
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192314
  CLC： R289; R284.1; R22; R2-031
- Published：20 December 2019，
  
  Published Online：27 August 2019，
  
  Received：04 February 2019，
- 稿件说明：
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Chao GUO, Shu-zhen JIAO, Chao XU, et al. Quality Relevance of Standard Decoction and Different Drying Methods Products for Gentianae Macrophyllae Radix Granules Based on HPLC Fingerprint. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):118-123(2019)
DOI：

Chao GUO, Shu-zhen JIAO, Chao XU, et al. Quality Relevance of Standard Decoction and Different Drying Methods Products for Gentianae Macrophyllae Radix Granules Based on HPLC Fingerprint. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(24):118-123(2019) DOI： 10.13422/j.cnki.syfjx.20192314.

摘要

目的：

建立秦艽标准汤剂及其不同方法干燥品的HPLC指纹图谱，对其质量相关性进行分析评价。

方法：

采用HPLC，InertSustain C

色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-磷酸为流动相进行梯度洗脱，检测波长240 nm，流速0.8 mL·min

－1

，柱温40 ℃。以对照指纹图谱及标准汤剂指纹图谱对秦艽标准汤剂不同方法干燥品从化学成分一致性、共有化学成分含量一致性以及主要化学成分含量和转移率等方面进行质量相关性分析。

结果：

建立秦艽标准汤剂对照指纹图谱，根据峰匹配数据确定15批秦艽标准汤剂指纹图谱中10个共有峰，从10个共有峰中指认出马钱苷酸，6′-

-葡萄糖基龙胆苦苷，獐牙菜苦苷，龙胆苦苷，獐牙菜苷5个化学成分。质量相关性分析结果表明，3种不同干燥方法制得干燥品水溶液与秦艽标准汤剂在化学成分种类及数量上一致性良好。但在共有化学成分含量及主要化学成分转移率方面，减压干燥所得干燥品与标准汤剂的质量低于喷雾干燥与冷冻干燥所得干燥品。

结论：

建立了秦艽标准汤剂-不同方法干燥品HPLC指纹图谱，通过化学成分、共有化学成分含量、主要化学成分含量及转移率3方面的质量相关性分析，全面反映了二者质量相关性情况。建议在秦艽配方颗粒制备的关键干燥工艺中采用喷雾干燥或冷冻干燥工艺，该研究为秦艽配方颗粒的制备工艺及质量控制提供了参考依据。

Abstract

Objective:

To establish the fingerprints of standard decoction of Gentianae Macrophyllae Radix-dried products by different methods

and to evaluate the quality correlation.

Method:

HPLC

InertSustain C

chromatographic column(4.6 mm×250 mm

5 μm)

Gradient elution was performed for the mobile phase of acetonitrile-phospho

detection wavelength at 240 nm and flow rate of 0.8 mL·min

-1

and column temperature was 40 ℃. The quality correlation analysis of different methods for different kinds of Gentianae Macrophyllae Radix standard decoction was carried out from the aspects of chemical composition consistency

common chemical composition consistency

main chemical composition content and transfer rate.

Result:

The control fingerprint of Gentianae Macrophyllae Radix standard decoction was established. According to the peak matching data

there were 10 common peaks in the fingerprint of 15 batches of Gentianae Macrophyllae Radix standard decoction.Among the 10 common peaks

5 chemical constituents of loganic acid

6′-

-glucosyl gentiopicroside

swertiamarin

gentiopicroside and swertia glycosides were identified. The results of quality correlation analysis showed that the three different drying methods were consistent with the chemical composition and quantity of Gentianae Macrophyllae Radix standard decoction. But in terms of the content consistency of common chemical components and the transfer rate of main chemical components

the quality correlation between the products obtained from vacuum drying and the standard decoction was lower than that obtained from spray drying and freeze-drying.

Conclusion:

The fingerprint of different method of Gentianae Macrophyllae Radix standard decoction was established. Through the analysis of the mass correlation of chemical composition consistency

common chemical composition content consistency

main chemical composition content and transfer rate

the mass correlation between them was comprehensively reflected. It is suggested that spray drying or freeze drying should be used for the key drying process of Gentianae Macrophyllae Radix granules. This study provides a reference for the preparation process and quality control of Gentianae Macrophyllae Radix granules.

关键词

秦艽配方颗粒标准汤剂减压干燥喷雾干燥冷冻干燥

Keywords

Gentianae Macrophyllae Radixformula granulestandard decoctionvacuum dryingspray dryingfreeze drying

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