Quality Control of Dynamic Volatile Components in Lonicerae Japonicae Flos and Lonicerae Flos

Hai-ying LI; Qi-meng FAN; Yu-ting HE; Peng HE; Min-cun WANG; Run-nan LIU; Zhuo ZHOU; Xue-bing QIAN; Xian-ping ZENG; Mei-feng XIAO; Fu-yuan HE

doi:10.13422/j.cnki.syfjx.20201047

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Quality Control of Dynamic Volatile Components in Lonicerae Japonicae Flos and Lonicerae Flos

Pharmacy Fundamentals | 更新时间：2020-12-10

- Quality Control of Dynamic Volatile Components in Lonicerae Japonicae Flos and Lonicerae Flos
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 22, Pages: 148-155(2020)
- 作者机构：
  
  湖南中医药大学药学院，中药成药性与制剂制备湖南省重点实验室，中医药超分子机理与数理特征化实验室，长沙 410208
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201047
  CLC： R22;R931;R28;C37;O657.7
- Received：30 November 2020，
  
  Published Online：04 February 2020，
  
  Published：20 November 2020
- 稿件说明：
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李海英,樊启猛,贺玉婷等.金银花、山银花动态挥发性成分的质量控制[J].中国实验方剂学杂志,2020,26(22):148-155.

LI Hai-ying,FAN Qi-meng,HE Yu-ting,et al.Quality Control of Dynamic Volatile Components in Lonicerae Japonicae Flos and Lonicerae Flos[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(22):148-155.
李海英,樊启猛,贺玉婷等.金银花、山银花动态挥发性成分的质量控制[J].中国实验方剂学杂志,2020,26(22):148-155. DOI： 10.13422/j.cnki.syfjx.20201047.

LI Hai-ying,FAN Qi-meng,HE Yu-ting,et al.Quality Control of Dynamic Volatile Components in Lonicerae Japonicae Flos and Lonicerae Flos[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(22):148-155. DOI： 10.13422/j.cnki.syfjx.20201047.

摘要

目的

运用分子连接性指数法与总量统计矩法控制中药质量，阐明金银花、山银花挥发性成分的稳定性与一致性。

方法

提取不同产地金银花、山银花挥发油进行GC-MS测定，采用电子轰击离子源，离子源温度230 ℃，检测范围

35~650；结合美国国家标准与技术局（NIST）05和ChemicalBook数据库定性分析挥发性成分，采用峰面积归一化法进行定量分析并计算总量统计矩参数和挥发性成分的零阶、一阶、二阶、三阶分子连接性指数。

结果

10批金银花、山银花各指纹图谱峰数目（RSD分别为28.5%，33.4%），总量零阶矩（RSD分别为55.5%，128.9%）和总量二阶矩（RSD分别为15.3%，21.5%）不稳定，表明挥发性成分种类与含量波动剧烈；但总量一阶矩（RSD分别为7.5%，8.8%）和零阶、一阶、二阶、三阶分子连接性指数较稳定（RSD分别在8.1%~10.3%，4.2%~5.5%），表明成分整体“印迹模板”相似。对各参数进行统计分析，发现金银花、山银花这2味药材挥发油的峰数、总量一阶矩及零阶、一阶、二阶、三阶分子连接性指数之间不具有显著性差异。

结论

以超分子气析“印迹模板”理论为指导，采用分子连接性指数法和总量统计矩参数联合表征中药体外成分“印迹模板”，可宏观控制中药质量的稳定性与一致性。

Abstract

Objective

The molecular connectivity index method and total statistical moment method were used to control the quality of traditional Chinese medicine （TCM）， and the stability and consistency of volatile components of Lonicerae Japonicae Flos and Lonicerae Flos were clarified.

Method

Volatile oils in Lonicerae Japonicae Flos and Lonicerae Flos from different producing areas was extracted for GC-MS determination with electron bombardment ion source， ion source temperature of 230 ℃， and detection range of

35-650. Then National Institute of Standards and Technology （NIST） 05 and ChemicalBook database were used for qualitative analysis of these volatile components， the peak area normalization method was used for quantitative analysis， and the total statistical moment parameters and the zero-order， first-order， second-order， third-order molecular connectivity indexes of the components were calculated.

Result

Number of peaks （RSD were 28.5%， 33.4%， respectively）， total zero-order moments （RSD were 55.5%， 128.9%， respectively） and total second-order moments （RSD were 15.3%， 21.5%， respectively） of 10 batches of Lonicerae Japonicae Flos and Lonicerae Flos were unstable， indicating that the types and contents of volatile components fluctuated sharply， but the total first-order moments （RSD were 7.5%， 8.8%， respectively） and the zero-order， first-order， second-order and third-order molecular connectivity indexes （RSD ranged from 8.1% to 10.3% and 4.2% to 5.5%， respectively） were relatively stable， indicating that the overall "imprinting template" of the components was similar. Statistical analysis of each parameter found that there were no significant differences in the number of peaks， total first-order moments and zero-order， first-order， second-order， third-order molecular connectivity indexes between volatile oils from Lonicerae Japonicae Flos and Lonicerae Flos.

Conclusion

Under the guidance of supramolecular gas evolution "imprinting template" theory， the molecular connectivity index method and total statistical moment parameters are used to jointly characterize the "imprinting template" of TCM components

in vitro

， which can control the stability and consistency of TCM quality.

关键词

Keywords

references

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