Analysis on Spectrum-effect Relationship of Anti-inflammatory Effect of Zhideke Granules Based on Chemical Pattern Recognition

Yu-shan ZHOU; Jie LIANG; Guang-qiang HUANG; Dong-fang HUANG; Jin-yu WEI; Ri-lan CHEN; Li-chun ZHAO

doi:10.13422/j.cnki.syfjx.20201646

您当前的位置：

首页 >

文章列表页 >

Analysis on Spectrum-effect Relationship of Anti-inflammatory Effect of Zhideke Granules Based on Chemical Pattern Recognition

Pharmacy Fundamentals | 更新时间：2020-12-10

- Analysis on Spectrum-effect Relationship of Anti-inflammatory Effect of Zhideke Granules Based on Chemical Pattern Recognition
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 22, Pages: 156-163(2020)
- 作者机构：
  
  1.广西中医药大学，南宁 530200
  2.广西中医药大学附属瑞康医院，南宁 530011
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201646
  CLC： R22;R28;R364.5;O657.7
- Received：14 March 2020，
  
  Published Online：27 May 2020，
  
  Published：20 November 2020
- 稿件说明：
移动端阅览
周昱杉,梁洁,黄光强等.基于化学模式识别方法分析止得咳颗粒的抗炎谱效关系[J].中国实验方剂学杂志,2020,26(22):156-163.

ZHOU Yu-shan,LIANG Jie,HUANG Guang-qiang,et al.Analysis on Spectrum-effect Relationship of Anti-inflammatory Effect of Zhideke Granules Based on Chemical Pattern Recognition[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(22):156-163.
周昱杉,梁洁,黄光强等.基于化学模式识别方法分析止得咳颗粒的抗炎谱效关系[J].中国实验方剂学杂志,2020,26(22):156-163. DOI： 10.13422/j.cnki.syfjx.20201646.

ZHOU Yu-shan,LIANG Jie,HUANG Guang-qiang,et al.Analysis on Spectrum-effect Relationship of Anti-inflammatory Effect of Zhideke Granules Based on Chemical Pattern Recognition[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(22):156-163. DOI： 10.13422/j.cnki.syfjx.20201646.

摘要

目的

研究止得咳颗粒HPLC指纹图谱与抗炎作用的谱效关系，探索其抗炎药效物质基础。

方法

采用HPLC建立10批止得咳颗粒的指纹图谱并进行色谱峰归属，流动相甲醇-0.2%磷酸水溶液梯度洗脱，检测波长254 nm。综合应用相似度评价，聚类分析（HCA），主成分分析（PCA）和正交偏最小二乘法-判别分析（OPLS-DA）评价止得咳颗粒批间质量差异。采用偏最小二乘法（PLS）将指纹图谱共有峰与二甲苯致小鼠耳肿胀和小鼠棉球肉芽肿的抑制率进行关联性分析，筛选出对抗炎药效作用贡献较大的色谱峰。

结果

止得咳颗粒HPLC指纹图谱标定了31个共有峰，10批样品相似度均≥0.992。HCA与PCA分析结果一致，均将样品分为3类；OPLS-DA结果发现15个成分是影响样品批间差异的主要标记物。不同批次止得咳颗粒样品均有不同程度的抗炎作用，PLS分析得出与抗炎作用呈正相关的色谱峰主要来自射干、黄芩，贡献较大的色谱峰为3，6，19，27~30号峰，其中28，30号峰依次为次野鸢尾黄素、汉黄芩素。

结论

HPLC指纹图谱结合化学模式识别方法可为系统评价止得咳颗粒整体质量提供参考。止得咳颗粒对小鼠急、慢性炎症反应有一定的抑制作用，抗炎作用是多种成分共同作用的结果，射干、黄芩对该制剂发挥抗炎作用具有显著意义。

Abstract

Objective

The aim of this study was to research the relationship between HPLC fingerprint and anti-inflammatory effect of Zhideke granules， and the substance basis of its anti-inflammatory effect was preliminary explored.

Method

The fingerprint of 10 batches of Zhideke granules were determined by HPLC， the mobile phase was consisted of methanol-0.2% phosphoric acid solution for gradient elution with a detection wavelength of 254 nm. Similarity analysis， hierarchical cluster analysis （HCA）， principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were used to evaluate the quality difference between batches of Zhideke granules. The correlation analysis between the common peaks and the inhibition rates of Zhideke granules on ear swelling and cotton ball granuloma in mice was carried out by partial least squares （PLS）， and the peaks greatly contributing to the anti-inflammatory effect were screened out.

Result

There were 31 common peaks in the HPLC fingerprint of Zhideke granules. The similarities of 10 batches samples were ≥0.992. The HCA and PCA analysis results were consistent， and the samples were divided into 3 categories. Combined with the OPLS-DA results， 15 components were the main markers affecting the differences of different batches of samples. Different batches of Zhideke granules differed in anti-inflammatory effect. The chromatographic peaks being positively correlated with the anti-inflammatory effect were mainly from Belamcandae Rhizoma and Scutellariae Radix， Chromatographic peaks 3， 6， 19， 27-30 had significant contribution to anti-inflammatory effect， of which peaks 28 and 30 were irisflorentin and wogonin.

Conclusion

HPLC fingerprint combined with chemical pattern recognition method can provide a reference for systematic evaluation of the overall quality of Zhideke granules. Zhideke granules has a certain inhibitory effect on acute and chronic inflammation in mice， and the anti-inflammatory effect is the result of the combined action of various ingredients， while Belamcandae Rhizoma and Scutellariae Radix have significant significance for the anti-inflammatory effect.

关键词

Keywords

references

辛静，李丽丽，凌爱思 . 止得咳冲剂治疗支气管哮喘急性发作期的临床观察［J］. 广西中医药大学学报， 2013 ， 16 （ 1 ）： 35 - 36 .

李戎，闫智勇，李文军，等 . 创建中药谱效关系学［J］. 中医教育， 2002 ， 21 （ 2 ）： 62 .

秦昆明，郑礼娟，沈保家，等 . 谱效关系在中药研究中的应用及相关思考［J］. 中国中药杂志， 2013 ， 38 （ 1 ）： 26 - 31 .

张小艺，刘久石，高石曼，等 . 中药谱效关系的研究方法及应用进展［J］. 中国中药杂志， 2019 ， 44 （ 20 ）： 4405 - 4411 .

白关亚 . 基于谱效关联研究青翘与老翘的药效物质基础［D］. 太原：山西省中医药研究院， 2017 .

潘杰，刘德胜，颜贵明 . 数据分析应用于中药谱效关系中的研究进展［J］. 中医药学报， 2018 ， 46 （ 4 ）： 119 - 122 .

李姣姣，于猛，高源，等 . 基于谱效关系的中药虎杖抗炎活性成分探讨［J］. 中国实验方剂学杂志， 2019 ， 25 （ 12 ）： 208 - 213 .

孙亮亮，吴剑，张叶，等 . 基于偏最小二乘回归分析法怀牛膝药材抗炎镇痛谱效关系［J］. 中成药， 2018 ， 40 （ 12 ）： 2786 - 2792 .

黄敏，黄国东，韦瑀龙，等 . 止得咳颗粒质量标准的研究［J］. 中国医药导报， 2018 ， 15 （ 30 ）： 106 - 110，182 .

孙如煜，李鹏跃，杜守颖，等 . 基于中药指纹图谱结合模式识别的清开灵硬胶囊批次间稳定性控制研究［J］. 中华中医药杂志， 2017 ， 32 （ 10 ）： 4629 - 4633 .

滕会会，杜守颖，白洁，等 . 基于中药指纹图谱结合化学模式识别的清开灵软/硬胶囊的成分分析［J］. 环球中医药， 2016 ， 9 （ 6 ）： 671 - 677 .

杨光 . 基于物质基础表征与新型化学模式识别方法的辛夷质量控制与药物动力学研究［D］. 上海：第二军医大学， 2017 .

姜建萍，王美琪，马雯芳，等 . 基于多种分析模式构建壮药滇桂艾纳香HPLC指纹图谱［J］. 中药材， 2018 ， 41 （ 1 ）： 124 - 128 .

李潮，于欢，温柔，等 . 江西不同产区车前子药材的HPLC指纹图谱及其多成分化学模式识别分析［J］. 中国实验方剂学杂志， 2019 ， 25 （ 15 ）： 161 - 167 .

沈梅芳，李小萌，单琪媛 . 薄荷化学成分与药理作用研究新进展［J］. 中华中医药学刊， 2012 ， 30 （ 7 ）： 1484 - 1487 .

泽仁拉姆，普珍，卓玛东智，等 . 荆芥的化学成分和药理作用［J］. 现代医药卫生， 2014 ， 30 （ 2 ）： 215 - 217 .

孙强，蒙艳丽，吴秉纯，等 . 桔梗化学成分及药理作用的研究概况［J］. 黑龙江中医药， 2017 ， 46 （ 4 ）： 64 - 65 .

颜美玲，杨柳，侯阿娇，等 . 柴胡化学成分及药理作用研究进展［J］. 中医药信息， 2018 ， 35 （ 5 ）： 103 - 109 .

刘洋，王四旺，唐志书 . 白前的现代研究与开发应用前景［J］. 西北药学杂志， 2015 ， 30 （ 6 ）： 768 - 770 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Improvement of Quality Standard of Shenwei Gubi Tablets and Changes of Transfer Rates of Its Quality Control Indicators in Production Based on Chemical Pattern Recognition Technology

Examination of Quality Characteristics of Rehmannia glutinosa Leaves by Different Fixation Methods Based on Fingerprint and Antioxidant Activity

Comparison Between Integrated and Traditional Decoction Pieces of Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine Based on Quality and Pharmacological Effect

Spectrum-effect Relationship Analysis Between HPLC Fingerprint and Immunomodulatory Activity of Zhenqi Fuzheng Granules

Quality Value Transmitting of Substance Benchmark of Xiebaisan

Related Author

Xiao-ling YU

Jiang LI

Jiao LI

Peng FU

Shuang PENG

Ling-yun CHEN

CUI Jinpeng

GENG Xiaotong

Related Institution

Kunming Municipal Hospital of Traditional Chinese Medicine

Yunnan Provincial Key Laboratory of Research on External Drug Delivery System and Preparation Technology in University，Yunnan University of Chinese Medicine

Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine， Henan University of Chinese Medicine

Xinyang Agriculture and Forestry University

Pharmacy Faculty，Hubei Engineering Technology Research Center of Chinese Medicine Processing，Hubei University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰