LEI Rui-xiang,YANG Bing-yue,WEI Yan-ni,et al.Allelopathic Effect of Water Extracts from Rhizosphere Soil of Three Medicinal Plants on Polygala tenuifolia Seeds and Their Seedlings[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(23):161-170.
LEI Rui-xiang,YANG Bing-yue,WEI Yan-ni,et al.Allelopathic Effect of Water Extracts from Rhizosphere Soil of Three Medicinal Plants on Polygala tenuifolia Seeds and Their Seedlings[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(23):161-170. DOI: 10.13422/j.cnki.syfjx.20202076.
Allelopathic Effect of Water Extracts from Rhizosphere Soil of Three Medicinal Plants on Polygala tenuifolia Seeds and Their Seedlings
To investigate the allelopathic effects of water extracts from rhizosphere soil of three medicinal plants
Rehmannia glutinosa
,
Pinellia ternata
and
Isatis indigotica
on seed germination and seedling growth of
Polygala tenuifolia
, screen the stubble varieties suitable for crop rotation with
P. tenuifolia
, and provide some scientific basis for continuous cropping obstacles of
P. tenuifolia.
Method
2
The bioassay method was used to study the effects of rhizosphere soil water extracts from three medicinal plants
Rehmannia glutinosa
,
Pinellia ternata
and
Isatis indigotica
at concentrations of 0.3,0.6,0.9 g·mL
-1
on the germination of
P. tenuifolia
seed and seedling growth.
Result
2
The rhizosphere soil water extracts of
Rehmannia glutinosa
and
Pinellia ternata
showed basically low-promotion and high-inhibition concentration effects on the final germination rate,germination potential,and germination index of
P. tenuifolia
seeds,while the water extract of
Isatis indigotica
showed significant allelopathic inhibition effect. All three rhizosphere soil water extracts showed significant allelopathic inhibition effects on the growth index of
P. tenuifolia
seedlings. Among them,the rhizosphere soil water extract of
Rehmannia glutinosa
showed lower inhibitory effect on the plant height and root length of
P. tenuifolia
seedlings than the other two water extracts. The photosynthetic pigment content,proline(Pro) content,and soluble sugar content of
P. tenuifolia
chinensis seedlings were the highest under 0.3 g·mL
-1
soil water extract of
Rehmannia glutinosa
, with relatively higher content of soluble protein, and relatively lower content of hydrogen oxide(H
2
O
2
). Under the treatment of 0.9 g·mL
-1
soil water extract of
Rehmannia glutinosa
,
P. tenuifolia
seedlings had the highest peroxidase(POD) and superoxide dismutase(SOD) activities,low catalase(CAT) activity,and lowest content of malondialdehyde(MDA).
Conclusion
2
Based on the comprehensive analysis of the above experimental data and allelopathic effects,the water extract of rhizosphere of
Rehmannia glutinosa
can promote the germination of
P. tenuifolia
seeds to a certain extent,and lay the foundation for seedling resistance to biochemical stress. Therefore,
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Related Author
Jian-ping GAO
Xiao-qiong ZHAO
Tai-shuai LIANG
Xue-lan ZHANG
Yue WANG
Zhi-hui ZHANG
Peng WU
Yue-li CUI
Related Institution
College of Pharmacy,Shanxi Medical University
Taiyuan Institute of Technology
School of Pharmacy,Shandong University of Traditional Chinese Medicine(TCM), Collaborative Innovation Center for Quality Control and Construction of Whole Industrial Chain of TCM in Universities of Shandong Province,Ji'nan