GC-MS Analysis of Volatile Oil of <italic style="font-style: italic">Notopterygium inchum </italic>and<italic style="font-style: italic"> Notopterygium franchetii</italic>

Wei-hao WANG; Li-xin YANG

doi:10.13422/j.cnki.syfjx.20202077

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GC-MS Analysis of Volatile Oil of Notopterygium inchum and Notopterygium franchetii

Pharmacy Fundamentals | 更新时间：2020-12-10

- GC-MS Analysis of Volatile Oil of Notopterygium inchum and Notopterygium franchetii
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 23, Pages: 153-160(2020)
- 作者机构：
  
  中国中医科学院中药研究所，北京 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202077
  CLC： R284.2;R289;R22;R2-031
- Received：12 November 2019，
  
  Published Online：02 September 2020，
  
  Published：05 December 2020
- 稿件说明：
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王维皓,杨立新.羌活挥发性成分GC-MS分析[J].中国实验方剂学杂志,2020,26(23):153-160.

WANG Wei-hao,YANG Li-xin.GC-MS Analysis of Volatile Oil of Notopterygium inchum and Notopterygium franchetii[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(23):153-160.
王维皓,杨立新.羌活挥发性成分GC-MS分析[J].中国实验方剂学杂志,2020,26(23):153-160. DOI： 10.13422/j.cnki.syfjx.20202077.

WANG Wei-hao,YANG Li-xin.GC-MS Analysis of Volatile Oil of Notopterygium inchum and Notopterygium franchetii[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(23):153-160. DOI： 10.13422/j.cnki.syfjx.20202077.

摘要

目的

气相色谱-质谱联用法

（

GC-MS

）

研究羌活及宽叶羌活的挥发性成分。

方法

收集羌活（

Notopterygium inchum

）及宽叶羌活（

N. franchetii

）10个批次的样品，包括羌活及宽叶羌活的栽培品各1个批次；四川和甘肃野生品8个批次。参照2015年版《中国药典》方法提取挥发油成分，采用GC-MS技术，分析不同批次样品的挥发油成分。

结果

鉴定出包括单萜、倍半萜以及非萜类共97个化学成分。化学成分相对含量的计算结果显示，

-蒎烯（25.39%~41.68%），

-蒎烯（19.34%~40.95%）和柠檬烯（4.59%~11.99%）为羌活及宽叶羌活挥发油的主要成分。野生羌活和栽培宽叶羌活中

-蒎烯的相对含量高于

-蒎烯；野生宽叶羌活中

-蒎烯相对含量高于

-蒎烯。贮存1年的羌活饮片中

-蒎烯的含量明显低于当年采收的样品。采用SIMCA-P+11.5软件进行偏最小二乘-判别分析，甘肃产羌活和四川产羌活差异不明显；当年采收的样品和贮存1年的样品明显聚为2类。

结论

该研究显示贮存对羌活挥发油中主要成分存在影响；研究还揭示了羌活的野生、栽培品挥发油成分的差异。为不同品种的羌活的挥发油药理活性比较提供化学研究基础。

Abstract

Objective

To study the volatile oil of

Notopterygium inchum

and

N. franchetii

by using gas chromatography-mass spectrometry （GC-MS）.

Method

Ten batches of

N. inchum

and

N. franchetii

were harvested from Sichuan and Gansu，including eight batches of wild samples， one batch of cultivated

N. inchum

and one batch of cultivated

N. franchetii

. Volatile oil was extracted according to the methods in

Chinese Pharmacopeia

， and the chemical constituents were analyzed with GC-MS technology.

Result

Ninety-seven chemical compounds were identified，including monoterpenes，sesquiterpenes and non-terpenes. The calculation results of the relative content of chemical components showed that

-pinene（25.39%-41.68%），

-pinene（19.34%-40.95%） and limonene（4.59%-11.99%） were main compounds in volatile oil of

N. inchum

and

N. franchetii

. Relative content of

-pinene was higher than that of

-pinene in wild

N. inchum

and cultivated

N. franchetii

， while relative content of

-pinene was higher than that of

-pinene in wild

N. franchetii

. Relative content of

-pinene in samples stored for one year was lower than that of newly harvested ones. The partial least squares discriminant analysis with SIMCA-P+11.5 software showed that the samples from Gansu and Sichuan provinces had no significant differences， and additionally，one-year-stored samples and newly-harvested samples were clustered into two groups.

Conclusion

This study showed that the storage had influence on the chemical constituents of volatile oil in

N. inchum

， and it also revealed the differences in volatile oil components of wild and cultivated

Notopterygium

. The above mentioned work provided chemical basis for the pharmacology comparison study of volatile oil from different kinds of

N. inchum

and

N. franchetii.

关键词

Keywords

references

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GC-MS Analysis of Volatile Oil of Notopterygium inchum and Notopterygium franchetii

DOI：10.13422/j.cnki.syfjx.20202077

摘要

Abstract

关键词

Keywords

references