Association Study Between Rhizoma <italic style="font-style: italic">Gastrodia elata</italic> Brown-rot and Soil Microbial Communities

Xin TANG; Jin-qiang ZHANG; Li-qing TENG; Tao ZHOU; Qing-song YUAN; Wei-ke JIANG; Lan-ping GUO; Jiao XU; Xiao WANG; Guang-wen ZHANG; Cheng PAN

doi:10.13422/j.cnki.syfjx.20202083

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Association Study Between Rhizoma Gastrodia elata Brown-rot and Soil Microbial Communities

Pharmacy Fundamentals | 更新时间：2020-12-10

- Association Study Between Rhizoma Gastrodia elata Brown-rot and Soil Microbial Communities
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 24, Pages: 153-160(2020)
- 作者机构：
  
  1.贵州中医药大学，贵阳 550025
  2.中国中医科学院中药资源中心，北京 100700
  3.山东省分析测试中心，济南 250014
  4.贵州乌蒙腾菌业有限公司，贵州大方 551600
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202083
  CLC： R284.2;R289;R22;R2-031
- Received：16 February 2020，
  
  Published Online：11 September 2020，
  
  Published：20 December 2020
- 稿件说明：
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唐鑫,张进强,滕力庆等.天麻褐腐病与土壤微生物菌群结构的关联分析[J].中国实验方剂学杂志,2020,26(24):153-160.

TANG Xin,ZHANG Jin-qiang,TENG Li-qing,et al.Association Study Between Rhizoma Gastrodia elata Brown-rot and Soil Microbial Communities[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(24):153-160.
唐鑫,张进强,滕力庆等.天麻褐腐病与土壤微生物菌群结构的关联分析[J].中国实验方剂学杂志,2020,26(24):153-160. DOI： 10.13422/j.cnki.syfjx.20202083.

TANG Xin,ZHANG Jin-qiang,TENG Li-qing,et al.Association Study Between Rhizoma Gastrodia elata Brown-rot and Soil Microbial Communities[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(24):153-160. DOI： 10.13422/j.cnki.syfjx.20202083.

摘要

目的

研究褐腐天麻与土壤微生物菌群的组成及变化，阐述天麻种植过程中天麻褐腐病与土壤微生物菌群之间的关系，为揭示天麻褐腐病的原因提供理论基础。

方法

采用内转录间隔区（ITS）和16S rDNA高通量测序技术，分别对健康、褐腐天麻及其周际土壤中真菌和细菌的微生物多样性、群落组成结构、群落结构相似性进行分析。

结果

与健康组相比，褐腐天麻及其土壤中真菌和细菌分类单元（OTUs）数目均有增加，且土壤中真菌、细菌丰富度和多样性显著增高，褐腐天麻真菌多样性显著降低，细菌多样性显著增高。在属分类水平上，健康天麻及其周际土壤中的优势真菌属为被孢霉属

Mortierella

，该属在褐腐天麻及其土壤中分别减少7.62%和15.75%，优势细菌属为慢生根瘤菌属

Bradyrhizobium

和

Burkholderia-Paraburkholderia

；褐腐天麻及其周际土壤中的优势真菌属为土赤壳属

Ilyonectria

，优势细菌属分别为沙雷氏菌属

Serratia

和慢生根瘤菌属

Bradyrhizobium

。

结论

褐腐天麻与其周际土壤中真菌菌群具有极高相似度，说明土壤微生物真菌群落变化在一定程度上造成天麻褐腐病的发生，其中褐腐天麻真菌群落组成中致病真菌土赤壳属

Ilyonectria

成为优势菌属，推测该菌属可能含有天麻褐腐病的致病菌。

Abstract

Objective

To study the microbial community composition and diversity of brown-rot

Gastrodia elata

and its surface soil，in order to explain the relationship between brown-rot

G. elata

and soil microflora in

G. elata

planting process and provide theoretical basis for revealing the reasons of

G. elata

brown-rot disease.

Method

Used internal transcribed spacer region（ITS） and 16S rDNA high-throughput sequencing technologies to detect the microbial diversity，community structure composition and community structure similarity of fungi and bacteria in healthy tuber，Brown-rot tuber，healthy soil and Brown-rot soil.

Result

Compared with health groups，the number of fungi and bacteria operational taxonomic units（OTUs） was increased in brown-rot

G. elata

and its soil， and the abundance and diversity of fungi and bacteria in brown-rot

G. elata

soil were significantly decreased. The diversity of fungi in the tubers of brown-rot

G. elata

was significantly reduced，while the diversity of bacteria was significantly decreased. At the genus level，

Mortierella

was dominant fungi genus in healthy tuber and healthy soil，which was reduced 7.62% and 15.75%

respectively in brown-rot tuber and brown-rot soil. And the dominant bacteria genus was

Bradyrhizobium

and

Burkholderia-Paraburkholderia

respectively.

Ilyonectria

was dominant fungi genus in brown-rot tuber and brown-rot soil，the dominant bacteria genus was

Serratia

and

Bradyrhizobium

respectively.

Conclusion

The fungal flora in the tuber of brown-rot

G. elata

had a very high degree of similarity to that in the surrounding soil. These results indicated that the change of soil microbial fungal community caused the occurrence of

G. elata

brown-rot disease to a certain extent. And the pathogenic fungal

Ilyonectria

was dominant genus in fungi community of brown-rot tuber and brown-rot soil.

Ilyonectria

may have the main

G. elata

brown-rot disease pathogen.

关键词

Keywords

references

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Association Study Between Rhizoma Gastrodia elata Brown-rot and Soil Microbial Communities

DOI：10.13422/j.cnki.syfjx.20202083

摘要

Abstract

关键词

Keywords

references