Genetic Diversity Analysis of <italic style="font-style: italic">Pogostemon cablin</italic> Based on AFLP Markers

Xiao-ying LIU; Jia-hui LI; Yu-ting YANG; Han-jing YAN; Hong-yi ZHANG; Meng-ling HE

doi:10.13422/j.cnki.syfjx.20202312

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Genetic Diversity Analysis of Pogostemon cablin Based on AFLP Markers

更新时间：2021-12-03

- Genetic Diversity Analysis of Pogostemon cablin Based on AFLP Markers
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 4, Pages: 152-158(2021)
- 作者机构：
  
  广东药科大学中药学院，国家中医药管理局岭南药材生产与开发重点研究室，中药材国家现代农业产业技术体系广州综合试验站（CARS-21-16），广州 510006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202312
  CLC： R284.2;R289;R22;R2-031
- Received：04 April 2020，
  
  Published Online：22 October 2020，
  
  Published：20 February 2021
- 稿件说明：
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刘晓莹,李嘉惠,杨钰婷等.基于AFLP分子标记的广藿香遗传多样性分析[J].中国实验方剂学杂志,2021,27(04):152-158.

LIU Xiao-ying,LI Jia-hui,YANG Yu-ting,et al.Genetic Diversity Analysis of Pogostemon cablin Based on AFLP Markers[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(04):152-158.
刘晓莹,李嘉惠,杨钰婷等.基于AFLP分子标记的广藿香遗传多样性分析[J].中国实验方剂学杂志,2021,27(04):152-158. DOI： 10.13422/j.cnki.syfjx.20202312.

LIU Xiao-ying,LI Jia-hui,YANG Yu-ting,et al.Genetic Diversity Analysis of Pogostemon cablin Based on AFLP Markers[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(04):152-158. DOI： 10.13422/j.cnki.syfjx.20202312.

摘要

目的

采用扩增片段长度多态性（AFLP）分子标记方法研究广藿香的种质遗传多样性。

方法

采用12对引物对14个品种共212个样本进行AFLP分析；利用POPGENE 32，Arlequinver 3.5，MEGA7，NTSYSpc 2.10e等生物学分析软件进行多态性参数计算、主坐标分析以及聚类分析。

结果

12对引物共扩增出2 238个位点，其中多态性位点有2 226个，多态位点百分率为99.38%；在种群间，有效等位基因数（

）为1.365 6±0.066 3，Nei's基因多样性指数（

）为0.220 7±0.036 4，Shannon多态性信息指数（

）为0.343 7±0.050 2；在种群内，

为1.118 5±0.038 7，

为0.071 3±0.023 0，

为0.109 4±0.035 0；分子方差学分析（AMOVA）表明广藿香的总变异有71.57%来源于种群间，28.43%来源于种群内；聚类分析可将14个种群分为4大类。

结论

AFLP分子标记结果显示，广藿香种间具有比较丰富的遗传多样性，种内遗传多样性相对较小，种间遗传分化显著，可为广藿香后续的优良种质筛选等研究提供参考依据。

Abstract

Objective

This paper aims to study the genetic diversity of

Pogostemon cablin

by amplified fragment length polymorphism （AFLP） markers.

Method

The 12 pairs of primers were used for AFLP analysis of 212 samples from 14 varieties，and biological analysis software such as POPGENE 32，Arlequinver 3.5，MEGA 7 and NTSYSpc 2.10e were used for polymorphism parameter calculation，principal coordinate analysis and cluster analysis.

Result

A total of 2 238 loci were amplified by 12 pairs of primers. 2 226 of them were polymorphic loci， accounting for 99.38%. At the inter-population level，the values of effective alleles（

），Nei's gene diversity index（

），Shannon polymorphic information index（

） were 1.365 6±0.066 3， 0.220 7±0.036 4， and 0.343 7±0.050 2，respectively；and 1.118 5±0.038 7，0.071 3±0.023 0，0.109 4±0.035 0，respectively at the intra-population level. Analysis of molecular variance（AMOVA）showed that 71.57% of the total variation of

P. cablin

was of inter-population nature， and 28.43% was of intra-population nature. The 14 populations could be divided into four groups by cluster analysis.

Conclusion

The results of AFLP molecular markers showed that abundant genetic diversity was present at inter-population level of

P. cablin

，however，relatively low at intra-population level； the genetic differentiation at the inter-population level was significant，which could provide a reference for the subsequent study of good germplasm selection of

P. cablin

关键词

Keywords

references

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Genetic Diversity Analysis of Pogostemon cablin Based on AFLP Markers

DOI：10.13422/j.cnki.syfjx.20202312

摘要

Abstract

关键词

Keywords

references