Scientific Analysis of Processing of Pinelliae Rhizoma by Ancient Hot Water Washing Method

Dong-ying LI; Kai-xi CUI; He MENG; Xiao-guang ZHU; Jian-ping YI

doi:10.13422/j.cnki.syfjx.20202355

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Scientific Analysis of Processing of Pinelliae Rhizoma by Ancient Hot Water Washing Method

Pharmacy Fundamentals | 更新时间：2021-03-29

- Scientific Analysis of Processing of Pinelliae Rhizoma by Ancient Hot Water Washing Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 7, Pages: 127-133(2021)
- 作者机构：
  
  北京中研同仁堂医药研发有限公司中药复方新药开发国家工程研究中心，北京 100079
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202355
  CLC： R22;R943.1;R28;O657.7
- Received：14 August 2020，
  
  Published Online：24 September 2020，
  
  Published：05 April 2021
- 稿件说明：
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李东影,崔凯茜,孟贺等.半夏古法“汤洗”炮制的科学性分析[J].中国实验方剂学杂志,2021,27(07):127-133.

LI Dong-ying,CUI Kai-xi,MENG He,et al.Scientific Analysis of Processing of Pinelliae Rhizoma by Ancient Hot Water Washing Method[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(07):127-133.
李东影,崔凯茜,孟贺等.半夏古法“汤洗”炮制的科学性分析[J].中国实验方剂学杂志,2021,27(07):127-133. DOI： 10.13422/j.cnki.syfjx.20202355.

LI Dong-ying,CUI Kai-xi,MENG He,et al.Scientific Analysis of Processing of Pinelliae Rhizoma by Ancient Hot Water Washing Method[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(07):127-133. DOI： 10.13422/j.cnki.syfjx.20202355.

摘要

目的

评价经典名方中半夏“汤洗”炮制的科学性和可行性，为相关经典名方的开发提供参考。

方法

基于古代“汤洗”炮制建立水洗半夏炮制方法，通过单因素试验优选工艺条件，测定炮制前后水分、灰分、浸出物，对其炮制前后质量变化、总酸含量（以琥珀酸计）以及高效液相色谱法（HPLC）指纹图谱进行比对，并进行家兔眼刺激试验，以评价水洗半夏上述指标及刺激性变化情况。

结果

水洗半夏炮制方法为加4倍量80 ℃热水反复清洗10次，洗至水清澈，切开断面透心，口尝无麻感或者微麻。水洗半夏的水分平均值9.34%，灰分平均值1.71%，浸出物平均值4.22%，质量下降率平均值7.49%，总酸质量分数下降率平均值43.31%，水洗炮制前后HPLC指纹图谱发现各成分均有所减少，但并无新增色谱峰，其中9号峰（腺苷）减少较为明显。家兔眼刺激试验结果表明水洗半夏浸液未出现明显眼结膜刺激性，水洗后毒性明显降低。

结论

建立的半夏“汤洗”炮制方法稳定可行，半夏水洗后的浸提液对家兔眼结膜无刺激性。

Abstract

Objective

To evaluate the scientificity and feasibility of processing of Pinelliae Rhizoma by hot water washing （Tangxi）， and to provide reference for the development of related famous classical formulas.

Method

Processing method of Pinelliae Rhizoma washed by hot water was established based on ancient Tangxi processing method， and the process conditions were optimized by single factor tests. The weight， moisture， ash， extract， total acid （calculated by succinic acid） contents and high performance liquid chromatography （HPLC） fingerprint of Pinelliae Rhizoma were compared before and after processing. In addition， the rabbit eye irritation test was conducted to evaluate the toxicity changes.

Result

The processing method of Pinelliae Rhizoma washed by hot water was as following：washed by 4 times the amount of hot water at 80 ℃ for 10 times until clear water， transfused cross-section after incision， no or slight numbness in the mouth. The average moisture， ash， extract contents of Pinelliae Rhizoma washed by hot water were 9.34%， 1.71% and 4.22%， respectively. After being processed， the decline rates of weight and total acid content of Pinelliae Rhizoma were 7.49% and 43.31%. The HPLC fingerprint of Pinelliae Rhizoma before and after washing showed a decrease in all components， but there was no new chromatographic peak， and peak 9 （adenosine） reduced significantly. The results of rabbit eye irritation test showed that there was no obvious eye conjunctival irritation after washing， indicating that the toxicity of Pinelliae Rhizoma decreased obviously after washing.

Conclusion

The established method of Pinelliae Rhizoma by Tangxi processing is stable and feasible， the aqueous extract of Pinelliae Rhizoma has no obvious eye conjunctival irritation after washing.

关键词

Keywords

references

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