Analysis of Effective Terpenoids in Xinjiang<italic style="font-style: italic"> Pleurotus ferulae</italic> Against Esophageal Carcinoma by UPLC-Q/TOF-MS Technique

Abulikemu MAIHEMUTI; Lei WANG; Jing LI; Aihaiti AIHEMAITIJIANG; Jun LIU

doi:10.13422/j.cnki.syfjx.20210149

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Analysis of Effective Terpenoids in Xinjiang Pleurotus ferulae Against Esophageal Carcinoma by UPLC-Q/TOF-MS Technique

Pharmacy Fundamentals | 更新时间：2021-04-20

- Analysis of Effective Terpenoids in Xinjiang Pleurotus ferulae Against Esophageal Carcinoma by UPLC-Q/TOF-MS Technique
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 10, Pages: 93-101(2021)
- 作者机构：
  
  新疆大学生命科学与技术学院，乌鲁木齐 830046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210149
  CLC： R22;R857.3;R28;R73
- Received：09 September 2020，
  
  Published Online：23 November 2020，
  
  Published：20 May 2021
- 稿件说明：
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买合木提·阿布里克木,王磊,李静等.基于UPLC-Q/TOF-MS技术的新疆阿魏菇抗食管癌有效萜类组分分析[J].中国实验方剂学杂志,2021,27(10):93-101.

MAIHEMUTI Abulikemu,WANG Lei,LI Jing,et al.Analysis of Effective Terpenoids in Xinjiang Pleurotus ferulae Against Esophageal Carcinoma by UPLC-Q/TOF-MS Technique[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):93-101.
买合木提·阿布里克木,王磊,李静等.基于UPLC-Q/TOF-MS技术的新疆阿魏菇抗食管癌有效萜类组分分析[J].中国实验方剂学杂志,2021,27(10):93-101. DOI： 10.13422/j.cnki.syfjx.20210149.

MAIHEMUTI Abulikemu,WANG Lei,LI Jing,et al.Analysis of Effective Terpenoids in Xinjiang Pleurotus ferulae Against Esophageal Carcinoma by UPLC-Q/TOF-MS Technique[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):93-101. DOI： 10.13422/j.cnki.syfjx.20210149.

摘要

目的

探究新疆阿魏菇中抗食管癌活性的有效萜类成分。

方法

经人食管癌Eca109细胞活性筛选，将阿魏菇醇提物乙酸乙酯萃取相（PFEP-E）通过硅胶柱和制备型薄层色谱技术进行分离纯化，采用超高效液相色谱-串联四极杆-飞行时间质谱法（UPLC-Q/TOF-MS）技术，结合Metlin，MassBank，PubChem等在线网络数据库及相关文献对有效洗脱部位进行成分鉴定及含量分析。

结果

硅胶柱石油醚-乙酸乙酯（3∶1）洗脱部位（Fr

2-3∶1

）对人食管癌Eca109细胞增殖的抑制活性最强，在正离子模式下分析鉴定出了24种萜类化合物，在负离子模式下分析鉴定出了28种萜类化合物，共52种，均为首次在该食用菌中分离得到；在Fr

2-3∶1

组分中总萜类成分质量分数62.88 mg·g

-1

，其包括2种单萜，5种倍半萜，15种二萜和30种三萜类，分别占总萜类含量的1.32%，12.04%，47.55%，39.09%。二萜类和三萜类是阿魏菇萜类的主要组成成分，占总萜类的86.64%。二萜类以赤霉素为主，占总二萜类的79.70%，三萜类以灵芝酸类为主，占总三萜类的29.25%。四甲基偶氮唑蓝（MTT）试验结果表明，赤霉素A

和赤霉素A

的抗食管癌活性较弱，而丝石竹皂苷元和齐墩果酸具有较强的抗食管癌活性。

结论

新疆阿魏菇抗食管癌有效萜类是以灵芝酸类为主的三萜类化合物，可为其作为抗肿瘤药物的开发以及功能性食品的研制提供理论依据，有助于有效提升阿魏菇的附加产值。

Abstract

Objective

To discover the effective terpenoids in Xinjiang

Pleurotus ferulae

with the activity of anti-esophageal carcinoma.

Method

By screening the activity of human esophageal cancer Eca109 cells， the ethyl acetate extract phase of

P. ferulae

ethanol extract （PFEP-E） was separated and purified by silica gel chromatography and preparative thin layer chromatography. Ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry （UPLC-Q/TOF-MS） combined with online network databases such as Metlin， MassBank， PubChem and related literature were used to identify the effective elution sites and analyze their contents.

Result

The elution fraction （Fr

2-3∶1

） of petroleum ether-ethyl acetate （3∶1） on the silica gel column had the strongest inhibitory activity on the proliferation of human esophageal cancer Eca109 cells. The component analysis showed that 24 effective terpenoids were identified under positive ion mode， and 28 effective terpenoids were identified under negative ion mode， a total of 52 terpenoids were identified， which were isolated from this edible fungus for the first time. Content of total terpenoids in Fr

2-3∶1

were 62.88 mg·g

-1

， including 2 monoterpenoids， 5 sesquiterpenoids， 15 diterpenoids and 30 triterpenoids， accounting for 1.32%， 12.04%， 47.55%， 39.09% of the total terpenoids， respectively. Diterpenoids and triterpenoids were the main components of the effective terpenoids in

P. ferulae

， accounting for 86.64% of the total terpenoids. Gibberellins were the main diterpenoids， accounting for 79.70% of the total diterpenoids， triterpenoids were mainly ganoderic acids， accounting for 29.25% of the total triterpenoids. The results of methyl thiazolyl tetrazolium （MTT） test showed that gibberellin A

and gibberellin A

had weak anti-esophageal cancer activity， while gypsogenin and oleanolic acid had strong anti-esophageal cancer activity.

Conclusion

The effective terpenoids of

P. ferulae

against esophageal cancer are triterpenoids mainly composed of ganoderic acids， which can provide theoretical basis for the development of terpenoids of

P. ferulae

as anti-tumor drugs and the development of functional foods， and help to effectively improve the additional output value of

P. ferulae

关键词

Keywords

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Analysis of Effective Terpenoids in Xinjiang Pleurotus ferulae Against Esophageal Carcinoma by UPLC-Q/TOF-MS Technique

DOI：10.13422/j.cnki.syfjx.20210149

摘要

Abstract

关键词

Keywords

references