Regulatory Effect of <italic style="font-style: italic">Polygala tenuifolia</italic> and Licorice-simmered <italic style="font-style: italic">P. tenuifolia</italic> on Learning and Memory， HPA Axis Function and Neurotransmitters in Rats with Heart-kidney Imbalance Insomnia

Xin ZHAO; Yue-li CUI; Peng WU; Zhi-hui ZHANG; Yue WANG; Xue-lan ZHANG

doi:10.13422/j.cnki.syfjx.20210151

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Regulatory Effect of Polygala tenuifolia and Licorice-simmered P. tenuifolia on Learning and Memory， HPA Axis Function and Neurotransmitters in Rats with Heart-kidney Imbalance Insomnia

Pharmacy Fundamentals | 更新时间：2021-05-17

- Regulatory Effect of Polygala tenuifolia and Licorice-simmered P. tenuifolia on Learning and Memory， HPA Axis Function and Neurotransmitters in Rats with Heart-kidney Imbalance Insomnia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 11, Pages: 147-154(2021)
- 作者机构：
  
  山东中医药大学药学院，山东省高校中药质量控制与全产业链建设协同创新中心，济南 250355
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210151
  CLC： R22;R256.23;R28;R943.1
- Received：18 September 2020，
  
  Published Online：23 November 2020，
  
  Published：05 June 2021
- 稿件说明：
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赵鑫,崔月莉,吴鹏等.远志与炆远志对心肾不交失眠大鼠学习记忆，HPA轴功能及神经递质的调控作用[J].中国实验方剂学杂志,2021,27(11):147-154.

ZHAO Xin,CUI Yue-li,WU Peng,et al.Regulatory Effect of Polygala tenuifolia and Licorice-simmered P. tenuifolia on Learning and Memory， HPA Axis Function and Neurotransmitters in Rats with Heart-kidney Imbalance Insomnia[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(11):147-154.
赵鑫,崔月莉,吴鹏等.远志与炆远志对心肾不交失眠大鼠学习记忆，HPA轴功能及神经递质的调控作用[J].中国实验方剂学杂志,2021,27(11):147-154. DOI： 10.13422/j.cnki.syfjx.20210151.

ZHAO Xin,CUI Yue-li,WU Peng,et al.Regulatory Effect of Polygala tenuifolia and Licorice-simmered P. tenuifolia on Learning and Memory， HPA Axis Function and Neurotransmitters in Rats with Heart-kidney Imbalance Insomnia[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(11):147-154. DOI： 10.13422/j.cnki.syfjx.20210151.

摘要

目的

比较远志与炆远志对心肾不交型失眠大鼠学习记忆、下丘脑-垂体-肾上腺轴（HPA轴）功能及神经递质的调控作用。

方法

多因素共同刺激致大鼠心肾不交失眠模型，造模结束后，给药组灌胃给予远志与炆远志提取物（给药剂量8 g·kg

-1

·d

-1

），正常组和模型组给予等体积生理盐水，连续灌胃7 d。采用Morris水迷宫检测各组大鼠学习记忆能力的变化；酶联免疫吸附测定法（ELISA）测定各组大鼠血清中促肾上腺皮质激素释放激素（CRH），促肾上腺皮质激素（ACTH），皮质醇（CORT）水平；超高效液相色谱-串联质谱法（UPLC-MS/MS）测定大鼠下丘脑中5-羟色胺（5-HT），多巴胺（DA），

-氨基丁酸（GABA）及谷氨酸（Glu）的含量。

结果

与正常组比较，模型组大鼠空间学习记忆能力下降，在目标象限停留的次数和时间明显减少（

0.05，

0.01）；血清中CORT，CRH，ACTH水平显著升高（

0.01）；下丘脑组织中GABA，DA，5-HT含量显著降低（

0.01），Glu含量显著升高（

0.01）。与模型组比较，远志与炆远志组大鼠空间学习记忆能力提高，在目标象限停留的次数和时间显著增加（

0.05，

0.01）；血清中CORT，CRH，ACTH水平明显降低（

0.05，

0.01）；下丘脑组织中GABA，DA，5-HT含量明显升高（

0.05，

0.01），Glu含量明显降低（

0.05）；炆远志组各指标恢复程度均优于远志组。

结论

远志与炆远志均可提高心肾不交型失眠大鼠的学习记忆能力，改善HPA轴功能，调节中枢神经递质水平，从而起到安神益智的功效，且炆远志作用优于远志。

Abstract

Objective

To compare the regulatory effects of

Polygala tenuifolia

and licorice-simmered

P. tenuifolia

on learning and memory， hypothalamus-pituitary-adrenal axis （HPA axis） function and neurotransmitters in rats with heart-kidney imbalance insomnia.

Method

The rat model of insomnia induced by multi-factor stimulation was established. After the model being made， the administration groups were given the extracts of

P. tenuifolia

and licorice-simmered

P. tenuifolia

by gavage （dose of 8 g·kg

-1

·d

-1

）， while the normal group and model group were given the same volume of normal saline for 7 days. Morris water maze was used to detect the changes of learning and memory ability of rats in each group. Enzyme-linked immunosorbent assay （ELISA） was used to determine the levels of corticotropin-releasing hormone （CRH）， adrenocorticotropic hormone （ACTH） and cortisol （CORT） in serum of rats from each group. The contents of 5-hydroxytryptamine （5-HT）， dopamine （DA），

-aminobutyric acid （GABA） and glutamic acid （Glu） in the hypothalamus of rats were determined simultaneously by ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS）.

Result

Compared with the normal group， the spatial learning and memory ability of rats in the model group

was decreased， the times and time of staying in target quadrant were significantly reduced （

0.05，

0.01）， the levels of CORT， CRH and ACTH in serum were significantly increased （

0.01）， the contents of GABA， DA， 5-HT in hypothalamus tissue were significantly decreased （

0.01）， and the content of Glu was significantly increased （

0.01）. Compared with the model group， the spatial learning and memory ability of rats in the

P. tenuifolia

group and licorice-simmered

P. tenuifolia

group

were increased， the times and time of staying in the target quadrant were significantly increased （

0.05，

0.01）， the levels of CORT， CRH and ACTH in serum were significantly decreased （

0.05，

0.01）， the contents of GABA， DA and 5-HT in hypothalamus tissue were significantly increased （

0.05，

0.01）， and the content of Glu was significantly decreased （

0.05）. The recovery degree of each index in the licorice-simmered

P. tenuifolia

group was better than that in the

P. tenuifolia

group.

Conclusion

Both

P. tenuifolia

and licorice-simmered

P. tenuifolia

can improve the learning and memory ability， improve the function of HPA axis， regulate the level of central neurotransmitters， and have the effect of calming the mind and improving the intelligence of rats with heart-kidney imbalance insomnia. The effect of licorice-simmered

P. tenuifolia

is better than that of

P. tenuifolia.

关键词

Keywords

references

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Regulatory Effect of Polygala tenuifolia and Licorice-simmered P. tenuifolia on Learning and Memory， HPA Axis Function and Neurotransmitters in Rats with Heart-kidney Imbalance Insomnia

DOI：10.13422/j.cnki.syfjx.20210151

摘要

Abstract

关键词

Keywords

references