Effect Mechanism of Total Flavonoid of Mori Cortex Combined with Total Saponins of Anemarrhenar Rhizoma on Hyperlipidemia Rats with Osteoporosis

Ju-ling XING; Yuan-xia DANG; Fen LIU; Meng FENG; Wei-min LI; Xin-xin ZHOU

doi:10.13422/j.cnki.syfjx.20210237

您当前的位置：

首页 >

文章列表页 >

Effect Mechanism of Total Flavonoid of Mori Cortex Combined with Total Saponins of Anemarrhenar Rhizoma on Hyperlipidemia Rats with Osteoporosis

更新时间：2021-12-03

- Effect Mechanism of Total Flavonoid of Mori Cortex Combined with Total Saponins of Anemarrhenar Rhizoma on Hyperlipidemia Rats with Osteoporosis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 2, Pages: 37-43(2021)
- 作者机构：
  
  广州中医药大学中药学院，广州 510006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210237
  CLC： R2-0;R289;R972+.6
- Received：20 July 2020，
  
  Published Online：26 November 2020，
  
  Published：20 January 2021
- 稿件说明：
移动端阅览
邢菊玲,党院霞,刘芬等.桑白皮总黄酮联合知母总皂苷改善高脂血症伴骨质疏松症大鼠的作用机制[J].中国实验方剂学杂志,2021,27(02):37-43.

XING Ju-ling,DANG Yuan-xia,LIU Fen,et al.Effect Mechanism of Total Flavonoid of Mori Cortex Combined with Total Saponins of Anemarrhenar Rhizoma on Hyperlipidemia Rats with Osteoporosis[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(02):37-43.
邢菊玲,党院霞,刘芬等.桑白皮总黄酮联合知母总皂苷改善高脂血症伴骨质疏松症大鼠的作用机制[J].中国实验方剂学杂志,2021,27(02):37-43. DOI： 10.13422/j.cnki.syfjx.20210237.

XING Ju-ling,DANG Yuan-xia,LIU Fen,et al.Effect Mechanism of Total Flavonoid of Mori Cortex Combined with Total Saponins of Anemarrhenar Rhizoma on Hyperlipidemia Rats with Osteoporosis[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(02):37-43. DOI： 10.13422/j.cnki.syfjx.20210237.

摘要

目的

探讨桑白皮总黄酮联合知母总皂苷对高脂血伴骨质疏松症大鼠的改善作用及其可能的机制。

方法

40只SPF级雄性SD大鼠适应性喂养7 d后，随机分为正常组、模型组、骨化三醇组（45 ng·kg

-1

），桑白皮总黄酮-知母总皂苷1∶2组（0.6 g·kg

-1

+0.4 g·kg

-1

）和桑白皮总黄酮-知母总皂苷2∶1组（1.2 g·kg

-1

+0.2 g·kg

-1

）。除正常组外，其余各组大鼠高脂饲喂造模9周；正常组与模型组灌胃给予生理盐水，其他组灌胃给予相应药物；给药12周后，除正常组外，其余各组给药的同时肌肉注射糖皮质激素，给药22周后测定各组大鼠的体质量，生化法检测血清中总胆固醇（TC），甘油三酯（TG），低密度脂蛋白胆固醇（LDL-C），骨钙素（BGP）及骨碱性磷酸酶（BALP）的含量；苏木素-伊红（HE）染色观察大鼠胫骨病理形态变化；实时荧光定量聚合酶链式反应（Real-time PCR）检测大鼠骨组织中过氧化物酶体增殖物激活受体

（PPAR

），Runt相关转录因子2（Runx2） mRNA的表达水平；免疫荧光法检测大鼠PPAR

，Runx2的表达。

结果

与正常组比较，模型组大鼠体质量显著升高（

0.01），血清中TC，TG，LDL-C含量显著增加（

0.01）；与模型组比较，桑白皮总黄酮-知母总皂苷1∶2组和桑白皮总黄酮-知母总皂苷2∶1组大鼠体质量显著降低（

0.01），血清中TC，TG，LDL-C含量显著降低（

0.01），BGP，BALP含量显著升高（

0.01）。HE染色结果表明，与正常组比较，模型组大鼠胫骨脂肪空泡增多，成骨细胞数量减少；与模型组比较，桑白皮总黄酮-知母总皂苷1∶2组和桑白皮总黄酮-知母总皂苷2∶1组大鼠胫骨脂肪空泡减小，成骨细胞数量增加。免疫荧光与Real-time PCR结果表明，与正常组比较，模型组大鼠Runx2表达减少，PPAR

表达增加（

0.01），与模型组比较，桑白皮总黄酮-知母总皂苷1∶2组和桑白皮总黄酮-知母总皂苷2∶1组上调Runx2的表达，下调PPAR

的表达（

0.05，

0.01）。

结论

桑白皮总黄酮联合知母总皂苷可上调Runx2，下调PPAR

mRNA和蛋白的表达，从而影响了血液中TG和TC代谢，达到对骨质疏松的治疗作用，为临床防治高脂血症伴骨质疏松症的治疗提供了实验依据。

Abstract

Objective

To explore the improvement effect of total flavonoids of Mori Cortex combined with total saponins of Anemarrhena Asphodeloide on hyperlipidemia rats with osteoporosis and its possible mechanism.

Method

The 40 SPF male SD rats were adaptively fed for 7 days， and then randomly divided into normal group， model group， calcitriol group （45 ng·kg

-1

）， total flavonoids of Mori Cortex and total saponins of Anemarrhena Asphodeloide 1∶2 group （0.6 g·kg

-1

+0.4 g·kg

-1

） and 2∶1 group （1.2 g·kg

-1

+0.2 g·kg

-1

）. Except for the normal group， rats in the other groups were fed with high fat for 9 weeks， the normal group and the model grouotal flavonoids of total flavonoids of Mori Cortex and total saponins of Anemarrhena Asphodeloip were given normal saline by gavage， and the other groups were given corresponding drugs by gavage， after 12 weeks of administration， except for the normal group ， the other groups were given intramuscular injection of glucocorticoids at the same time. After 22 weeks of administration， the weight of rats with total flavonoids from Mori Cortex combined with total saponins of Anemarrhena Asphodeloide was measured. Serum levels of total cholesterol （TC）， triglyceride （TG）， low density lipoprotein cholesterol （LDL-C）， osteocalcin （BGP） and bone alkaline phosphatase （BALP） were determined by biochemical assay. Hematoxylin-eosin （HE） staining to observe the pathological changes of rat tibia. Real-time fluorescent quantitative polymerase chain reaction （Real-time PCR） was used to detect the expression levels of peroxisomal proliferators activate the receptor gamma（PPAR

） and Runt-related transcription factor 2 （Runx2） mRNA in rat bone tissue， immunofluorescence was used to detect the expression of PPAR

and Runx2 in rats.

Result

Compared with normal group， the body mass of rats in model group was significantly increased （

0.01）， and the contents of TC， TG， and LDL-C in the serum were significantly increased （

0.01）. Compared with model group， the body weight of rats in thet total flavonoids of Mori Cortex and total saponins of Anemarrhena Asphodeloide 1∶2 group and 2∶1 group were significantly reduced （

0.01）， and the contents of TC， TG， and LDL-C in the serum were significantly reduced （

0.01）， the content of BGP and BALP increased （

0.01）. HE staining results showed that compared with the normal group， the tibia fat vacuoles of the model group increased， and the number of osteoblasts decreased， compared with the model group， the total flavonoids of the Mori Cortex and the flavonoids-total saponins of Anemarrhena Asphodeloide 1∶2 group and 2∶1 group decreased in tibia fat vacuoles and increased the number of osteoblasts， the results of immunofluorescence and Real-time PCR showed that， compared with normal group， the expression of Runx2 in the model group decreased and the expression of PPAR

increased （

0.01）. Compared with model group， the total flavonoids of Mori Cortex-total saponins 1∶2 group and the total flavonoids of Mori Cortex-total saponins 2∶1 Group up-regulated the expression of Runx2 and down-regulated the expression of PPAR

（

0.05，

0.01）.

Conclusion

The total flavonoids of Mori Cortex combined with the total saponins of Anemarrhena Asphodeloide up-regulated Runx2 and down-regulated the expression of PPAR

mRNA and protein， thereby affecting the metabolism of TG and TC in the blood， achieving a therapeutic effect on osteoporosis， provides experimental basis for the clinical prevention and treatment of hyperlipidemia with osteoporosis.

关键词

Keywords

references

董晓菲 . 高脂血症大鼠种植体周围骨组织中的基因共表达网络研究及机制初探［D］. 济南：山东大学， 2018 .

BOUHLALI E ， HMIDANI A ， BOURKHIS B ， et al . Effect of phoenix dactylifera seeds（dates）extract in triton WR-1339 and high fat diet induced hyperlipidaemia in rats：a comparison with simvastatin ［J］. J Ethnopharmacol ， 2020 ， 259 ： 112961 .

施洋，樊官伟，候宝林，等 . 桑白皮化学成分及药理作用研究进展［J］. 辽宁中医杂志， 2020 ， 11 （ 25 ）： 1 - 9 .

王慧娟 . 桑白皮黄酮类化合物抗骨质疏松活性及作用机制的研究［D］. 贵阳：贵州大学， 2019 .

孙勇 . 桂枝芍药知母汤加减联合扶他林软膏治疗膝关节骨性关节炎的疗效观察［J］. 临床医药文献电子杂志， 2019 ， 6 （ 32 ）： 169 - 172 .

翁丽丽，陈丽，宿莹，等 . 知母化学成分和药理作用［J］. 吉林中医药， 2018 ， 38 （ 1 ）： 90 - 92 .

李艳，曹美群，陶文聪，等 . 黄芪散对高脂饮食诱导肥胖大鼠肝脏内质网应激信号通路的影响［J］. 中国实验方剂学杂志， 2019 ， 25 （ 21 ）： 6 - 11 .

王芳，陈珺，曾煦欣，等 . 黄芪散对肥胖模型大鼠胫骨骨结构的研究［J］. 中国骨质疏松杂志， 2019 ， 25 （ 5 ）： 632 - 637 .

王芳，曾煦欣，徐彬，等 . 黄芪散对T2DM大鼠体脂系数和脂肪组织MCP-1、UCP-1、PRDM16、Cidea mRNA表达的影响［J］. 中华中医药学刊 2018 ， 36 （ 10 ）： 2345 - 2348 .

党院霞 . 桑白皮联合知母对高脂血症伴骨质疏松症的作用研究［D］. 广州：广州中医药大学， 2019 .

段志涛，高英，霍文杰，等 . 桑白皮中总黄酮含量测定方法研究［J］. 中国实验方剂学杂志， 2013 ， 19 （ 7 ）： 133 - 135 .

李子雯，贾丹，党院霞，等 . 百合知母汤总皂苷调节5-HT功能治疗抑郁症的作用机制［J］. 中国实验方剂学杂志， 2018 ， 24 （ 19 ）： 131 - 138 .

国家药典委员会 . 中华人民共和国药典［M］. 一部.北京：中国医药科技出版社， 2015 ： 298 .

张悦，李运峰 . 骨质疏松症动物模型研究进展［J］. 中国骨质疏松杂志， 2020 ， 26 （ 1 ）： 152 - 156 .

WANG X Y ， GONG L J ， HUANG J M ， et al . Pinocembrin alleviates glucocorticoid-induced apoptosis by activating autophagy via suppressing the PI3K/Akt/mTOR pathway in osteocytes ［J］. Eur J Pharmacol ， 2020 ， 880 ： 173212 .

何明，凃长春，黄起壬，等 . Lee's指数用于评价成年大鼠肥胖程度的探讨［J］. 中国临床药理学与治疗学杂志， 1997 ， 14 （ 3 ）： 171 .

张志达，沈耿杨，任辉，等 . 木通皂苷D促进糖皮质激素环境下小鼠骨髓间充质干细胞成骨分化［J］. 中国骨质疏松杂志， 2020 ， 26 （ 4 ）： 529 - 533 .

刘飞祥，林子璇，徐道明，等 . 左归丸防治骨质疏松症的动物模型和评价方法［J］. 中国实验方剂学杂志， 2019 ， 25 （ 18 ）： 16 - 23 .

张莉莉，李玉坤 . 大鼠骨组织中IRS-2 mRNA水平与2型糖尿病性骨质疏松发病的关系［J］. 河北医科大学学报， 2019 ， 40 （ 6 ）： 637 - 641 .

倪扬 . 阿仑膦酸钠联合他汀类药物对老年高脂血症合并骨质疏松患者的疗效［J］. 湖南师范大学学报：医学版， 2019 ， 16 （ 1 ）： 38 - 40 .

KOZISEK T ， HAMANN A ， NGUYEN A ， et al . High-throughput screening of clinically approved drugs that prime nonviral gene delivery to human Mesenchymal stem cells ［J］. J Biol Eng ， 2020 ， 14 ： 16 .

江园 . PEMF通过PPAR γ -Axin2-Wnt信号轴调节GIOP大鼠骨髓脂质代谢及其影响骨代谢的机制研究［D］. 重庆：重庆医科大学， 2016 .

王保平，贾红蔚，郑纺，等 . 糖皮质激素诱导骨质疏松的机制和治疗进展［J］. 实用医学杂志， 2018 ， 34 （ 14 ）： 2290 - 2292 .

张朔，王英骥 . PPARs激动剂与骨质疏松症［J］. 中国骨质疏松杂志， 2015 ， 21 （ 6 ）： 761 - 768 .

廖丽姿，刘磊，田卫东 . PPAR γ 天然激动剂的研究进展［J］. 现代生物医学进展， 2009 ， 9 （ 7 ）： 1381 - 1383 .

SONG Y ， DU Z ， REN M ， et al . Association of gene variants of transcription factors PPARgamma，RUNX2，Osterix genes and COL2A1，IGFBP3 genes with the development of osteonecrosis of the femoral head in Chinese population ［J］. Bone ， 2017 ， 101 ： 104 - 112 .

FARSANI Z H ， BANITALEBI E ， FARAMARZI M ， et al . Effects of different intensities of strength and endurance training on some osteometabolic miRNAs，Runx2 and PPARgamma in bone marrow of old male wistar rats ［J］. Mol Biol Rep ， 2019 ， 46 （ 2 ）： 2513 - 2521 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical Efficacy of Zhuyuwan in Treatment of Hyperlipidemia with Syndrome of Phlegm Turbidity and Obstruction

Zhuyuwan in Treatment of Hyperlipidemia Complicated with Carotid Atherosclerosis： A Randomized Controlled Trial

Mechanism of Wendantang in Intervention of ApoE^-/- Hyperlipidemic Mice Based on Liver Metabolomics

Anti-osteoporosis Effect of Isorhamnetin： A Review

Polygonati Odorati Rhizoma Polysaccharides Ameliorate Hyperlipidemia in Mice by Regulating Gut Microbiota

Related Author

YANG Lele

LUO Danmei

CHEN Jiao

ZHANG Xiaobo

SONG Wei

ZHU Wenyu

ZHOU Xin

LI Xueping

Related Institution

Hospital of Chengdu University of Traditional Chinese Medicine （TCM）

Chengdu University of TCM

Chengdu University of Traditional Chinese Medicine（TCM）

Hospital of Chengdu University of TCM

Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory/Jiangxi Province Key Laboratory of Biological Etiologies of TCM/First-Level Discipline Platform of Chinese and Western Integrative Medicine/Experimental Animal and Scientific Technology Center of Jiangxi University of Chinese Medicine/School of Pharmacy， Jiangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰