Efficacy of Fufang Huangbaiye Tuji on Inflammation on Skin with Deep Ⅱ Degree Burn and Its Dose-effect Relationship

Chang-shuan XIAO; Ya-ping LIU; Kui SUN; Jing-zhe YANG; Hong-yu CHEN

doi:10.13422/j.cnki.syfjx.20210313

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Efficacy of Fufang Huangbaiye Tuji on Inflammation on Skin with Deep Ⅱ Degree Burn and Its Dose-effect Relationship

更新时间：2021-12-03

- Efficacy of Fufang Huangbaiye Tuji on Inflammation on Skin with Deep Ⅱ Degree Burn and Its Dose-effect Relationship
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 4, Pages: 88-94(2021)
- 作者机构：
  
  承德医学院附属医院，河北承德 067000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210313
  CLC： R284.2;R289;R22;R2-031
- Received：02 September 2020，
  
  Published Online：30 December 2020，
  
  Published：20 February 2021
- 稿件说明：
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肖长栓,刘娅平,孙奎等.复方黄柏液涂剂对深Ⅱ° 烧伤创面炎症反应的影响及量效关系[J].中国实验方剂学杂志,2021,27(04):88-94.

XIAO Chang-shuan,LIU Ya-ping,SUN Kui,et al.Efficacy of Fufang Huangbaiye Tuji on Inflammation on Skin with Deep Ⅱ Degree Burn and Its Dose-effect Relationship[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(04):88-94.
肖长栓,刘娅平,孙奎等.复方黄柏液涂剂对深Ⅱ° 烧伤创面炎症反应的影响及量效关系[J].中国实验方剂学杂志,2021,27(04):88-94. DOI： 10.13422/j.cnki.syfjx.20210313.

XIAO Chang-shuan,LIU Ya-ping,SUN Kui,et al.Efficacy of Fufang Huangbaiye Tuji on Inflammation on Skin with Deep Ⅱ Degree Burn and Its Dose-effect Relationship[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(04):88-94. DOI： 10.13422/j.cnki.syfjx.20210313.

摘要

目的

观察复方黄柏液涂剂不同剂量外用对深Ⅱ° 烧伤创面愈合过程中炎症反应的影响。

方法

将2019年6月至2020年3月承德医学院附属医院烧伤整形科确诊的深Ⅱ° 烧伤火毒伤津证患者120例，采用随机数字表法分为对照组（1%体表面积创面外用碘伏溶液35 mL），低剂量治疗组（1%体表面积创面外用复方黄柏液涂剂17.5 mL）和高剂量治疗组（1%体表面积创面外用复方黄柏液涂剂35 mL），每组各40例，每日换药1次。观察各组患者入院时及治疗后创面炎症反应；各组治疗后4 d，观察创面病理学变化，免疫组织化学法测定创面组织核转录因子-

B亚基p65亲和肽（NF-

B p65）的表达情况；治疗后第4，10，21天各组细菌培养并计数，分别按照酶联免疫吸附法测定创面组织中白细胞介素（IL）-２，IL-８水平及肿瘤坏死因子-

（TNF-

）水平。

结果

入院时各组创面炎症反应程度重且无明显差别，治疗后减轻程度与治疗时间呈正相关，同时相点创面炎症反应对照组

低剂量治疗组

高剂量治疗组。对照组于第4天出现细菌生长，低剂量及高剂量治疗组于第10天出现细菌生长，以金黄色葡萄球菌和铜绿假单胞菌检出率最高。与对照组比较，低剂量及高剂量治疗组在治疗后4 d创面组织NF-

B p65吸光度明显降低（

0.05），在治疗后10

，

21 d细菌计数明显减少（

0.05），在治疗后4，10，21 d创面组织中IL-２，IL-８及TNF-

水平明显降低（

0.05），且上述指标低剂量与高剂量组之间差异具有统计学意义（

0.05）。光镜下低剂量及高剂量治疗组均较对照组组织水肿减轻，炎症细胞浸润减少，且高剂量组表现更显著。

结论

复方黄柏液涂剂外用能减少深Ⅱ° 烧伤创面细菌生长，可能通过抑制炎症介质的产生及释放减轻创面炎症反应，且存在一定的量效关系，值得临床推广应用。

Abstract

Objective

To observe the effect of different doses of Fufang Huangbaiye Tuji asin the treatment onof the inflammatory response in healing process for of skin with deep Ⅱ degree burn. Methods in healing process.

Methods

The 120 cses patients with deep Ⅱ degree burn of fire-toxin injuring fluid syndrome diagnosed in the affiliated hospital of Chengde Medical University between June 2019 and March 2020 were randomly divided into control group，low -dose treatment group and high -dose treatment group，with 40 cases in each group and once. They got a dressing change perevery day. Control group was locally administered with lodophor solution 35 mL per 1% on the body surface area. Low-dose treatment group was locally administered with compound cortex phellodendri fluid 17.5 mL per 1% on the body surface area，while high-dose treatment group was locally administered with compound cortex phellodendri fluid 35 mL per 1% on the body surface area. Observe theThe inflammatory reaction of wound surface in each group onwas observed at admission and after treatment. The pathological changes of each groupsgroup were observed， and determination of nuclear factor kappa-B（NF-

B） p65 expression inon the wound surface was determined by immunohistochemistry on the 4th day after the treatment. The levels of interleukin（IL）-2，IL-8 and tumor necrosis factor（TNF）-α in wound tissue were measured with ELISA and Bacterial culture and count were performed in each group on the 4

，10

and 21

daydays after treatment. The levels of IL-2，IL-8 and TNF-α in wound tissue were measured with ELISA.

Results

There was no significant difference in the degree of wound inflammation in each group at admission，and the degree of relief after treatment was positively correlated with the treatment time. At the simultaneous phase point，the inflammatory reaction was severest in control group，which was followed by low-dose treatment group and high-dose treatment group. Bacterial growth were observed on the 4

day in control group，which was found in low-dose and high-dose treatment groups on the 10

day，the detection rates of Staphylococcus aureus and Pseudomonas aeruginosa were the highest. Compared with control group，the mean integrated optical density of NF-

B p65 in wound tissue decreased markedly in low-dose and high-dose treatment groups on the 4th day after treatment（

0.05），the bacterial count decreased significantly in low-dose and high-dose treatment groups on the 10

and 21

days after treatment（

0.05），and the levels of IL-2，IL-8 and TNF-

in wound tissue decreased markedly in low-dose and high-dose treatment groups on the 4

，10

and 21

days after treatment（

0.05），with statistically significant differences between low-dose and high-dose treatment groups（

0.05）. Histopathological examination showed that inflammatory granulocytes and edema were improved in low-dose and high-dose treatment groups compared with control group，with a more significant performance in high-dose treatment group.

Conclusion

The external application of compound cortex phellodendri fluid can reduce thebacterial growth of bacteria in on the wound surface，which may reduce the inflammatory reaction by inhibiting the production and release of inflammatory mediators，with a certain dose-effcteffect relationship，and is worth clinical promotion.

关键词

Keywords

references

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