Analysis on Material Basis and Mechanism Difference of Antibacterial Effect of <italic style="font-style: italic">Coptis chinensis </italic>and Its Flower Stalk Based on Secondary Metabolites and Network Pharmacology

Hui YANG; Hong-mei ZHU; Rui-si WU; Cai-hong LI; Ying-jun HE; Yu WEI; Yu-ling XU

doi:10.13422/j.cnki.syfjx.20210611

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Analysis on Material Basis and Mechanism Difference of Antibacterial Effect of Coptis chinensis and Its Flower Stalk Based on Secondary Metabolites and Network Pharmacology

Data Mining | 更新时间：2021-04-20

- Analysis on Material Basis and Mechanism Difference of Antibacterial Effect of Coptis chinensis and Its Flower Stalk Based on Secondary Metabolites and Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 10, Pages: 133-141(2021)
- 作者机构：
  
  成都大学，成都 610106
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210611
  CLC： R285;R289;R22;R2-031;R33
- Received：12 August 2020，
  
  Published Online：09 March 2021，
  
  Published：20 May 2021
- 稿件说明：
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杨慧,朱红梅,伍蕊嗣等.基于次生代谢产物和网络药理学探索黄连及黄连花薹抗菌作用的物质基础及机制差异[J].中国实验方剂学杂志,2021,27(10):133-141.

YANG Hui,ZHU Hong-mei,WU Rui-si,et al.Analysis on Material Basis and Mechanism Difference of Antibacterial Effect of Coptis chinensis and Its Flower Stalk Based on Secondary Metabolites and Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):133-141.
杨慧,朱红梅,伍蕊嗣等.基于次生代谢产物和网络药理学探索黄连及黄连花薹抗菌作用的物质基础及机制差异[J].中国实验方剂学杂志,2021,27(10):133-141. DOI： 10.13422/j.cnki.syfjx.20210611.

YANG Hui,ZHU Hong-mei,WU Rui-si,et al.Analysis on Material Basis and Mechanism Difference of Antibacterial Effect of Coptis chinensis and Its Flower Stalk Based on Secondary Metabolites and Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):133-141. DOI： 10.13422/j.cnki.syfjx.20210611.

摘要

目的

基于次生代谢产物与网络药理学探讨黄连及黄连花薹抗菌作用机制差异。

方法

基于超高液相色谱-质谱法（UPLC-MS/MS）检测平台，自建黄连及黄连花薹次生代谢产物数据库（MWDB），通过代谢物信息公共数据库以及多元统计分析相结合的手段研究了黄连花薹和黄连的次生代谢组差异，筛选含量相对较高的黄连花薹18个代谢产物和11个黄连代谢产物，借助BATMAN-TCM数据库获取成分作用靶点，于UniProt数据库中查询靶点对应的基因。通过GeneCards检索抗菌基因，使用Venny平台获得成分和抗菌的交集基因。通过DAVID进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）通路富集分析，预测其作用机制，并通过GraphPad Prism软件和OmicShare数据库绘制柱状图及高级气泡图使结果可视化。通过STRING数据库构建蛋白质-蛋白质相互作用（PPI）网络，Cytoscape 3.7.2软件构建成分-靶标-通路网络，对网络药理学分析结果对比二者抗菌差异性。

结果

通过网络药理学得到黄连发挥抗菌有效活性成分比黄连花薹少5个，抗菌靶点比黄连花薹多55个；预测黄连和黄连花薹对抗菌作用的共同成分为槲皮素和小檗碱；参与抗菌作用的不同关键基因有p38丝裂原活化蛋白激酶14（MAPK14），过氧化氢酶（CAT）；涉及的不同关键通路有疟疾及Toll样受体信号通路。

结论

黄连与黄连花薹主要通过多靶点、多通路的方式发挥抗菌作用，可为黄连和黄连花薹抗菌机制研究提供新的思路和线索，同时为黄连花薹资源的综合开发和合理应用提供了新的发展方向。

Abstract

Objective

To explore the difference in antibacterial mechanism between

Coptis chinensis

and

its

flower stalk based on secondary metabolites and network pharmacology.

Method

Based on the ultraperformance liquid chromatography mass spectrometry（UPLC-MS/MS） detection platform，the secondary metabolites database of

C. chinensis

and its flower stalk（MWDB） was built. The common database of metabolites information and the multivariate statistical analysis were used to study the differences of secondary metabolites between

C. chinensis

and its flower stalk and screen out 18 metabolites of

the

flower stalk and 11 metabolites of

C. chinensis

with a high content. BATMAN-TCM database was used to obtain the targets of component action，and their corresponding genes were inquired in the UniProt database. GeneCards was retrieved for antimicrobial genes，and the intersection genes of components and antimicrobials were obtained on Venny platform. Through DAVID gene ontology（GO） enrichment analysis and Kyoto encyclopedia of genes and genomes（KEGG） pathway enrichment analysis，the mechanism of its action was predicted，and the results were visualized through histogram and advanced bubble diagram drawn by GraphPad Prism software and OmicShare database. The protein-protein interaction（PPI） network was constructed by STRING， database and the component-target-pathway network was constructed by Cytoscape 3.7.2 software. The antibacterial differences were compared based on the results of network pharmacology analysis.

Result

Through network pharmacology，the antibacterial active components of

C. chinensis

were 5 fewer than that of the flower stalk，55 more antibacterial targets than that of the flower stalk； quercetin and berberine were predicted to be the common components of the antagonistic action of

C. chinensis

and the flower stalk. Key genes involved in antimicrobial action were p38 Mitogen-activated protein kinase 14（MAPK14），catalase（CAT）； malaria and Toll-like receptor signaling pathway were different key pathways involved in antimicrobial activity.

Conclusion

C. chinensis

and the flower stalk mainly exert the antibacterial effect in a multi-target and multi-pathway manner，which can offer new ideas and clues for the study of antibacterial mechanism of

C. chinensis

and the flower stalk，and provide a new development direction for the comprehensive development and rational application of the flower stalk resources.

关键词

Keywords

references

国家药典委员会 . 中华人民共和国药典：一部［M］. 北京：中国医药科技出版社， 2015 ： 303 - 305 .

王琳，杨阳，张一颖，等 . 中药抗菌作用文献研究现状［J］. 世界中西医结合杂志， 2017 ， 12 （ 12 ）： 1663 - 1665，1763 ．

魏华波，董洋，谭兵，等 . 黄连花薹化学成分和药理作用的研究进展［J］. 现代生物医学进展， 2014 ， 14 （ 27 ）： 5342 - 5344 .

伍利华，杨慧，杨俊莉，等 . 反相高效液相色谱法及紫外可见分光光度法测定黄连花薹的含量［J］. 时珍国医国药， 2019 ， 30 （ 6 ）： 1330 - 1332 .

许海玉，刘振明，付岩，等 . 中药整合药理学计算平台的开发与应用［J］. 中国中药杂志， 2017 ， 42 （ 18 ）： 3633 - 3688 ．

董培良，李慧，韩华 . 中药网络药理学的应用与思考［J］. 中国实验方剂学杂志， 2020 ， 26 （ 17 ）： 204 - 211 .

CHEN M ， ZHU J ， KANG J ， et al . Exploration in the mechanism of action of licorice by network pharmacology ［J］. Molecules ， 2019 ， 24 （ 16 ）： 2959 .

王伟姣，龙凌云，姜成君，等 . UPLC-MS/MS法测定减肥类保健食品中非法添加17种化学药的研究［J］. 中南药学， 2019 ， 17 （ 8 ）： 1255 - 1260 .

杨庆懿，杨柳，王凯，等 . 超高效液相色谱-串联质谱法同时测定保健食品中15种非法添加降糖化学药物［J］. 食品安全质量检测学报， 2019 ， 10 （ 7 ）： 2038 - 2042 .

张雯霞，冯敏，苗雨露，等 . 基于UPLC-Q-TOF-MS/MS技术的还贝止咳方化学成分分析［J］. 中国中药杂志， 2019 ， 44 （ 14 ）： 3022 - 3034 .

马莹，蔡媛，马晓晶，等 . 药用植物活性成分生物合成中P450的研究进展［J］. 药学学报， 2020 ， 55 （ 7 ）： 1573 - 1589 .

LAU W ， SATTELY E S . Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone ［J］. Science ， 2015 ， 349 （ 6253 ）： 1224 - 1228 .

CHEN W ， GAO Y ， XIE W ， et al . Genome-wide association analyses provide genetic and biochemical insights into natural variation in rice metabolism ［J］. Nature Genetics ， 2014 ， 46 ： 714 - 721 .

欧水平，沈必江，王森，等 . 中药“一体多用性”及新药用部位开发的探析［J］. 中国中医基础医学杂志， 2016 ， 22 （ 5 ）： 685 - 686，716 .

赵祥 . 炎症标志物CRP、TNF-α、IL-6、PCT与白细胞计数分析在肺部细菌感染中诊断价值分析［C］// 中国中西医结合学会 . 第一次全国中西医结合检验医学学术会议暨中国中西医结合学会检验医学专业委员会成立大会论文汇编： 2014 年卷. 北京：中国中西医结合学会， 2014 ： 5 .

问亚锋，金恂，金宝生，等 . CD64指数、血清降钙素原、IL-6及TNF-α鉴别细菌感染的临床价值［J］. 国际检验医学杂志， 2014 ， 35 （ 9 ）： 1183 - 1184 .

MATSUBARA A ， WASSON J C ， DONELAN S S ， et al . Isolation and characterization of the human AKT1 gene，identification of 13 single nucleotide polymorphisms（SNPs），and their lack of association with Type Ⅱ diabetes ［J］. Diabetologia ， 2001 ， 44 （ 7 ）： 910 - 913 .

ALWHAIBI A ， VERMA A ， ADIL M S ， et al . The unconventional role of Akt1 in the advanced cancers and in diabetes-promoted carcinogenesis ［J］. Pharmacol Res ， 2019 ， 145 ： 104270 .

陈桐莹，高丰禾，汪悦东，等 . 基于生物信息学探讨骨质疏松症和膝骨关节炎的关系［J］. 中国骨质疏松杂志， 2020 ， doi： 11.3701.R.20200728.1542.004 http://dx.doi.org/11.3701.R.20200728.1542.004 .

段海婧，龙晓宙，杜丽东，等 . 清肺排毒汤对三种冠状病毒感染的SARS、MERS和COVID-19的潜在共性作用及机制探讨［J］. 中药药理与临床， 2020 ， doi： 10.13412/j.cnki.zyyl.20200706.001 http://dx.doi.org/10.13412/j.cnki.zyyl.20200706.001 .

刘涛，赵灵丽，钟林江，等 . 基于网络药理学的复方当归注射液作用机制研究［J］. 成都大学学报：自然科学版， 2020 ， 39 （ 3 ）： 225 - 229 .

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Analysis on Material Basis and Mechanism Difference of Antibacterial Effect of Coptis chinensis and Its Flower Stalk Based on Secondary Metabolites and Network Pharmacology

DOI：10.13422/j.cnki.syfjx.20210611

摘要

Abstract

关键词

Keywords

references