Analysis of Volatile Organic Compounds in Rhizosphere Soil of <italic style="font-style: italic">Rehmannia glutinosa</italic> Under Intercropping <italic style="font-style: italic">Zea mays</italic> Based on GC-MS Metabonomics

Li GU; Xiao-sai TAO; Feng-qing WANG; Ming-jie LI; Chao-fei YANG; Rui-bin SUN; Cai-xia XIE; Zhong-yi ZHANG

doi:10.13422/j.cnki.syfjx.20210617

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Analysis of Volatile Organic Compounds in Rhizosphere Soil of Rehmannia glutinosa Under Intercropping Zea mays Based on GC-MS Metabonomics

Pharmacy Fundamentals | 更新时间：2021-04-20

- Analysis of Volatile Organic Compounds in Rhizosphere Soil of Rehmannia glutinosa Under Intercropping Zea mays Based on GC-MS Metabonomics
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 10, Pages: 102-108(2021)
- 作者机构：
  
  1.福建农林大学，福州 350002
  2.河南中医药大学，郑州 450046
  3.河南农业大学，郑州 450002
  4.河南省百疗怀药科技开发有限公司，河南焦作 454000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210617
  CLC： R284.2;R289;R22;R2-031;R33
- Received：21 February 2021，
  
  Published Online：18 March 2021，
  
  Published：20 May 2021
- 稿件说明：
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古力,陶晓赛,王丰青等.基于GC-MS代谢组学分析间作玉米下地黄根际土壤中挥发性有机物特征[J].中国实验方剂学杂志,2021,27(10):102-108.

GU Li,TAO Xiao-sai,WANG Feng-qing,et al.Analysis of Volatile Organic Compounds in Rhizosphere Soil of Rehmannia glutinosa Under Intercropping Zea mays Based on GC-MS Metabonomics[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):102-108.
古力,陶晓赛,王丰青等.基于GC-MS代谢组学分析间作玉米下地黄根际土壤中挥发性有机物特征[J].中国实验方剂学杂志,2021,27(10):102-108. DOI： 10.13422/j.cnki.syfjx.20210617.

GU Li,TAO Xiao-sai,WANG Feng-qing,et al.Analysis of Volatile Organic Compounds in Rhizosphere Soil of Rehmannia glutinosa Under Intercropping Zea mays Based on GC-MS Metabonomics[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):102-108. DOI： 10.13422/j.cnki.syfjx.20210617.

摘要

目的

分析地黄-玉米间作模式下地黄根际土壤挥发性有机物特征，筛选间作玉米下地黄根际土壤中特殊的信号物质，为地黄连作障碍中化感物质的研究提供依据。

方法

本实验以7~10月份玉米间作及地黄单作模式下的地黄根际土壤为研究对象，利用气相色谱-质谱法（GC-MS）分析其乙酸乙酯部位中的挥发性有机物，并应用SIMCA 14.1对数据进行主成分分析（PCA），聚类分析（HCA）及正交偏最小二乘法-判别分析（OPLS-DA）处理，筛选2种模式下挥发性有机物中潜在的差异成分。

结果

间作及单作模式下地黄根际土壤中的挥发性有机物种类主要为烃类、醇类、酯类、酮类、酰胺类、酸类等物质，其中间作模式下烃类、酯类、酰胺类成分的平均相对质量分数分别为58.46%，32.15%，5.42%，而单作模式下则分别为37.27%，36.11%，21.13%；PCA与HCA分析结果表明间作与单作两种模式下根际土壤乙酸乙酯部位的挥发性有机物特征可以明显地被分为两类；基于OPLS-DA分析的潜在差异成分筛选结果表明，从两种模式下的根际土壤中筛选到邻苯二甲酸二丁酯，（

）-9-油酸酰胺，间苯二甲酸二辛酯，邻苯二甲酸（2-丙基戊基）二酯，正二十六烷，二十八烷，正二十一烷等差异达到显著水平的几种挥发性有机物。

结论

地黄-玉米间作模式对地黄根际土壤中的挥发性有机物有一定的影响，筛选到的几种差异成分对地黄生长及质量特征的影响还有待于进一步研究。

Abstract

Objective

To explore the composition characteristics of rhizosphere soil under

Rehmannia glutinosa-Zea mays

intercropping model，and screen out special signal substances in rhizosphere soil of

R. glutinosa

under intercropping

Z. mays

， so as to provide the basis for the study of allelopathic substances in continuous cropping obstacle of

R. glutinosa

Method

In this experiment，rhizosphere soils of

R. glutinosa

under

Z. mays

intercropping and

R. glutinosa

single cropping models in July，August，September and October were taken as the research objects， and the volatile organic compounds in ethyl acetate fraction were analyzed by gas chromatography-mass spectrometry （GC-MS）. Principal component analysis （PCA）， hierachical cluster analysis （HCA） and orthogonal partial least squares discriminant analysis （OPLS-DA） analysis were performed on the data by SIMCA 14.1 to screen out potential differences in volatile organic compounds between the two models.

Result

The types of volatile organic compounds in intercropping and single cropping models were mainly hydrocarbons， alcohols， esters， ketones， amides， acids and other substances. Specifically， the average relative contents of hydrocarbons，esters and amides in intercropping model were 58.46%，32.15% and 5.42% respectively，while the relative contents of hydrocarbons，esters and amides in single cropping model were 37.27%，36.11% and 21.13%. The results of PCA and HCA showed that the characteristics of volatile organic compounds in the ethyl acetate fraction of rhizosphere soil under intercropping and single cropping models could be clearly divided into two categories，the screening results of potential differential components based on OPLS-DA analysis indicated that various components， such as dibutyl phthalate，（

）-9-oleamide，

-caryophyllene，dioctyl iso-phthalate， phthalate （2-propylamyl） diester，

-hexadecane，octodecane，

-heneicosane， were screened from rhizosphere soil under the two models.

Conclusion

The

R. glutinosa-Z. mays

intercropping model has certain effects on the volatile organic compounds in the rhizosphere soil of

R. glutinosa

，and the effect of the selected components on the growth and quality characteristics of

R. glutinosa

still need to be further studied.

关键词

Keywords

references

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Analysis of Volatile Organic Compounds in Rhizosphere Soil of Rehmannia glutinosa Under Intercropping Zea mays Based on GC-MS Metabonomics

DOI：10.13422/j.cnki.syfjx.20210617

摘要

Abstract

关键词

Keywords

references