<italic style="font-style: italic">Fusarium</italic> Wilt Changes Microbial Community Structure in Rhizosphere Soil of <italic style="font-style: italic">Chrysanthemum morifolium</italic>

Qiao-huan CHEN; Yu-huan MIAO; Tie-lin WANG; Lan-ping GUO; Da-hui LIU

doi:10.13422/j.cnki.syfjx.20210713

您当前的位置：

首页 >

文章列表页 >

Fusarium Wilt Changes Microbial Community Structure in Rhizosphere Soil of Chrysanthemum morifolium

Pharmacy Fundamentals | 更新时间：2021-05-17

- Fusarium Wilt Changes Microbial Community Structure in Rhizosphere Soil of Chrysanthemum morifolium
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 11, Pages: 180-186(2021)
- 作者机构：
  
  1.湖北中医药大学药学院，武汉 430065
  2.中国中医科学院中药资源中心，北京 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210713
  CLC： R284.2;R289;R22;R2-031;R33
- Received：16 February 2021，
  
  Published Online：29 March 2021，
  
  Published：05 June 2021
- 稿件说明：
移动端阅览
陈巧环,苗玉焕,王铁霖等.枯萎病对菊花根际土壤微生物群落结构的影响[J].中国实验方剂学杂志,2021,27(11):180-186.

CHEN Qiao-huan,MIAO Yu-huan,WANG Tie-lin,et al.Fusarium Wilt Changes Microbial Community Structure in Rhizosphere Soil of Chrysanthemum morifolium[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(11):180-186.
陈巧环,苗玉焕,王铁霖等.枯萎病对菊花根际土壤微生物群落结构的影响[J].中国实验方剂学杂志,2021,27(11):180-186. DOI： 10.13422/j.cnki.syfjx.20210713.

CHEN Qiao-huan,MIAO Yu-huan,WANG Tie-lin,et al.Fusarium Wilt Changes Microbial Community Structure in Rhizosphere Soil of Chrysanthemum morifolium[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(11):180-186. DOI： 10.13422/j.cnki.syfjx.20210713.

摘要

目的

探究患枯萎病菊花与健康菊花根际土壤微生物群落的差异。

方法

以患病菊花植株和健康菊花植株根际土壤为研究材料，采用高通量测序技术对患病植株和健康植株样本的细菌16S rDNA和真菌内部转录间隔区（ITS）基因进行序列测定并进行数据分析。

结果

枯萎病的发生降低了菊花根际土壤中细菌种群的丰富度和多样性程度，但对根际土壤中的真菌

-多样性无明显影响。菊花健康植株根际土壤细菌微生物中酸杆菌门、芽单胞菌门、硝化螺旋菌门占比高于患病植株，而变形菌门、拟杆菌门占比低于患病植株（

0.05）。菊花患病植株根际土壤中镰刀菌属（

Fusarium

）真菌占比分别为27.49%，14.53%，11.94%，而健康植株镰刀菌属真菌占比分别为0.47%，1.01%，0.67%。菊花患病植株根际土壤中会出现细菌性致病菌果胶杆菌属（

Pectobacterium

）和菊迪基氏菌属（

Dickeya

），而健康植株根际土壤中硝化类细菌、解毒类细菌、光合细菌等丰度高于患病植株。

结论

患有枯萎病地块菊花植株根际土壤微生物中细菌的物种多样性和丰富度降低，并大量富集镰刀菌属致病真菌和积累果胶杆菌属与菊迪基氏菌属致病细菌，而健康菊花植株根际土壤微生物有益菌占比明显高于患病植株。

Abstract

Objective

To explore the differences in rhizosphere microbial community structure between

Fusarium

wilt-infected and healthy

Chrysanthemum morifolium

plants

Method

The rhizosphere soils of diseased and healthy

C. morifolium

plants were sampled and subjected to high-throughput 16S ribosomal DNA （rDNA） and internal transcribed spacer （ITS） sequencing， to identify the microbial community structure including bacteria and fungi.

Result

Fusarium

wilt reduced the bacterial abundance and diversity but had no significant effect on fungal alpha-diversity.The proportions of Acidobacteria， Gemmatimonadetes， and Nitrospirae in rhizosphere soil of healthy

C.morifolium

plants were higher than those of diseased plants， while the proportions of Proteobacteria and Bacteroidetes were lower（

0.05）.

Fusarium

fungi accounted for 27.49%， 14.53%， and 11.94% in diseased plants whereas 0.47%， 1.01%， and 0.67% in healthy plants.Pathogenic bacteria

Pectobacterium

and

Dickeya

were enriched in rhizosphere soil of diseased plants. The abundances of nitrifying， detoxifying， and photosynthetic bacteria in rhizosphere soil of healthy plants were higher than those of diseased plants.

Conclusion

Fusarium

wilt reduces the bacterial richness and diversity and triggers the enrichment of massive

Fusarium

fungi，

Pectobacterium

， and

Dickeya

. The proportion of beneficial bacteria in rhizosphere soil of healthy plants is significantly higher than that of diseased plants.

关键词

Keywords

references

国家药典委员会 . 中华人民共和国药典：一部［M］. 北京：中国医药科技出版社， 2020 ： 323 - 324 .

瞿璐，王涛，董勇喆，等 . 菊花化学成分与药理作用的研究进展［J］. 现代药物与临床， 2015 ， 38 （ 1 ）： 98 - 104 .

SINGH P K ， KUMAR V . Fusarium wilt of Chrysanthemum –problems and prospects ［J］. Plant Pathol Quarantine ， 2014 ， 4 （ 1 ）： 33 - 42 .

王超，李刚，黄思杰，等 . 枯草芽胞杆菌菌肥对有机冬瓜根区土壤微生态的影响［J］. 微生物学通报， 2019 ， 46 （ 3 ）： 563 - 576 .

赵兰凤 . 生物有机肥对香蕉枯萎病及土壤微生物学特性的影响研究［D］. 广州：华南农业大学， 2016 .

NIU B ， PAULSON J N ， ZHENG X ， et al . Simplified and representative bacterial community of maize roots ［J］. PNAS ， 2017 ， 114 （ 12 ）： 2450 - 2459

柳少燕，陈捷胤，李蕾，等 . 拮抗菌与病原菌碳水化合物酶类比较分析［J］. 基因组学与应用生物学， 2013 ， 32 （ 1 ）： 97 - 104

梁银，张谷月，王辰，等 . 一株拮抗放线菌的鉴定及其对黄瓜枯萎病的生防效应研究［J］. 土壤学报， 2013 ， 50 （ 4 ）： 810 - 817

李雪萍 . 青藏高原青稞根腐类病害及其对根际土壤微生态的影响［D］. 兰州：甘肃农业大学， 2017 .

毛忠顺，龙月娟，朱书生，等 . 三七根腐病研究进展［J］. 中药材， 2013 ， 36 （ 12 ）： 2051 - 2054 .

刘凡 . 白术根腐病病原鉴定，生物学特性和防治研究［D］. 雅安：四川农业大学， 2012 .

王瑞，董林林，徐江，等 . 农田栽参模式中人参根腐病原菌鉴定与防治［J］. 中国中药杂志， 2016 ， 41 （ 10 ）： 1787 - 1791 .

SANTHANAM R ， LUU V T ， WEINHOLD A ， et al . Native rootassociated bacteria rescue a plant from a sudden-wilt disease that emerged during continuous cropping ［J］. Proc Natl Acad Sci ， 2015 ， 112 （ 36 ）： E5013 - E5020 .

BAKKER P A H M ， DOORNBOS R F ， ZAMIOUDIS C ， et al . Induced systemic resistance and the rhizosphere microbiome ［J］. Plant Pathol J ， 2013 ， 29 （ 2 ）： 136 - 143 .

徐娇，欧小宏，江维克，等 . 天麻-冬荪轮种模式对土壤微生物群落结构的影响［J］. 中国中药杂志， 2020 ， 45 （ 3 ）： 463 - 471 .

吕宁，石磊，刘海燕，等 . 生物药剂滴施对棉花黄萎病及根际土壤微生物数量和多样性的影响［J］. 应用生态学报， 2019 ， 30 （ 2 ）： 602 - 614 .

邓晓，李勤奋，武春媛，等 . 健康香蕉（ Musa paradisiaca ）植株与枯萎病患病植株根区土壤细菌多样性的比较研究［J］. 生态环境学报， 2015 ， 24 （ 3 ）： 402 - 408 .

伍文宪，黄小琴，张蕾，等 . 十字花科作物根肿病对根际土壤微生物群落的影响［J］. 生态学报， 2020 ， 40 （ 5 ）： 1532 - 1541 .

周芳，曹国璠，李金玲，等 . 药用植物连作障碍机制及其缓解措施研究进展［J］. 山地农业生物学报， 2019 ， 38 （ 3 ）： 67 - 72 .

吴林坤，黄伟民，王娟英，等 . 不同连作年限野生地黄根际土壤微生物群落多样性分析［J］. 作物学报， 2015 ， 41 （ 2 ）： 308 - 317 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Exogenous Fusarium oxysporum and Trichoderma viride on Soil Fungal Community of Panax ginseng

Effect of Different Fermentation Conditions on Fungal Community and Chemical Composition of Aurantii Fructus

Microbial Diversity and Physicochemical Properties of Rhizosphere Soil of Healthy and Diseased Andrographis paniculata

Application of GSS in Analysis of Endophytic Bacterial Diversity in Ligusticum chuanxiong

Effect of Different Cultivation Modes on Microbial Diversity and Secondary Metabolites of Rhizosphere Soil of Gentiana crassicaulis

Related Author

Jing FANG

Li-li WENG

Chun-ping XIAO

Di LI

Xue-qing YANG

YAN Zhihong

LIU Xiumei

GUAN Qiuyan

Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jiangxi Guxiang Jinyun Comprehensive Health Industry Co. Ltd.

Key Laboratory of Depression Animal Model Based on Traditional Chinese Medicine（TCM）Syndrome of Jiangxi Province Administration of TCM，Key Research Office for Evaluation of TCM Efficacy（Prevention and Treatment of Mental Disorders and Brain Diseases） of Jiangxi Province Administration of TCM， Jiangxi University of Chinese Medicine

Life Science and Technology School of Lingnan Normal University

State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Fusarium Wilt Changes Microbial Community Structure in Rhizosphere Soil of Chrysanthemum morifolium

DOI：10.13422/j.cnki.syfjx.20210713

摘要

Abstract

关键词

Keywords

references