YAN Hong-yuan,GONG Wen-ling,LIU Yin,et al.Analysis and Evaluation of Mineral Elements in Gastrodia elata with Different Specifications and Grades from Diverse Producing Areas[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(12):147-156.
YAN Hong-yuan,GONG Wen-ling,LIU Yin,et al.Analysis and Evaluation of Mineral Elements in Gastrodia elata with Different Specifications and Grades from Diverse Producing Areas[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(12):147-156. DOI: 10.13422/j.cnki.syfjx.20210719.
Analysis and Evaluation of Mineral Elements in Gastrodia elata with Different Specifications and Grades from Diverse Producing Areas
To study the distribution characteristics of mineral elements in
Gastrodia elata
samples
with different grades and specifications (variants) from diverse producing areas and their classification and identification evidences.
Method
2
Fourteen mineral elements in 31 batches of
Gastrodia elata
samples of different grades and specifications (variants) from diverse producing areas were determined by atomic absorption spectrophotometry, Mo-Sb colorimetry, and curcumin colorimetry, and then subjected to correlation analysis (CA), discriminant analysis (DA), and principal component analysis (PCA).
Result
2
The content of K, N, and P in
G. elata
was the highest, enabling them to serve as the nutritional limiting factors affecting its growth. The
G. elata
samples could be identified by the variation trend of elements (K
>
N
>
P
>
Mg
>
Ca
>
Fe
>
B
>
Zn
>
Mn
>
Cu
>
Ni
>
Cr
>
Pb
>
Cd). The comparison of
G. elata
samples from multiple producing areas showed that
G. elata
from Zhaotong has the highest P, Fe, and Cd content, that from Lijiang the highest K content, that form Luotian the highest Zn and Cr content, and that from Jinzhai the highest Cu and Pb content. The content of Mg, B, Pb, and Cr in
G. elata
f.
elata
was higher than that in
G. elata
f.
glauca
. It was found that the content of P, Cu, and Cd in commercially available
G. elata
products gradually increased with the decrease in the commercial grade, while that of Mg, Fe, B, and Ni mostly decreased. As revealed by CA, Fe was positively correlated with Mg, Cr, and B. The producing areas of
G. elata
samples could be effectively identified by DA with Cd, Pb, Cr, Cu, B, and Ni as the main variables, and the accuracy reached up to 85.71%. According to the PCA of mineral elements in
G. elata
f.
glauca
from Zhaotong, Yunnan Province, Fe, Cr, Mg, Cd, P, Mn, B, Pb, and Cu exerted a greater influence on
G. elata
.
Conclusion
2
The determination of mineral elements in
G. elata
samples contributes to identifying their authenticity and origin due to the easy operation, accurate results, and good stability.
ZHAO Y S , MA X F , FAN L L , et al . Origin of cultivated Polygala tenuifolia based on multi-element fingerprinting by inductively coupled plasma mass spectrometry [J]. Sci Rep , 2017 , 7 ( 1 ): 144 - 146 .
GAO F Y , XU Z H , WANG W Z , et al . A comprehensive strategy using chromatographic profiles combined with chemometric methods: application to quality control of Polygonum cuspidatum Sieb. et Zucc [J]. J Chromatogr A , 2016 , 1466 : 67 - 75 .
GRANATO D , SANTOS J S , ESCHER G B , et al . Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: a critical perspective [J]. Trends Food Sci Technol , 2018 , 72 : 83 - 90 .