Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by A<italic style="font-style: italic">β</italic><sub>1-42</sub>

Li-xin CAO; Cheng-jun DONG; Yan YANG; Yu-qing LIANG; Yu-xi LUO; Zhi-yan XIE; Jie-mei WANG; Bo XIA; Wen-bin WU

doi:10.13422/j.cnki.syfjx.20210802

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Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by Aβ_1-42

Classic Prescriptions | 更新时间：2021-04-20

- Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by Aβ_1-42
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 10, Pages: 23-30(2021)
- 作者机构：
  
  成都中医药大学附属医院，成都 610075
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20210802
  CLC： R2-0;R22;R285.5;R289;R33
- Received：01 January 2021，
  
  Published Online：18 March 2021，
  
  Published：20 May 2021
- 稿件说明：
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曹蠡馨,董秤均,杨燕等.黄连解毒汤对Aβ_1-42诱导AD大鼠学习记忆能力及胆碱能系统的影响[J].中国实验方剂学杂志,2021,27(10):23-30.

CAO Li-xin,DONG Cheng-jun,YANG Yan,et al.Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by Aβ_1-42[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):23-30.
曹蠡馨,董秤均,杨燕等.黄连解毒汤对Aβ_1-42诱导AD大鼠学习记忆能力及胆碱能系统的影响[J].中国实验方剂学杂志,2021,27(10):23-30. DOI： 10.13422/j.cnki.syfjx.20210802.

CAO Li-xin,DONG Cheng-jun,YANG Yan,et al.Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by Aβ_1-42[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(10):23-30. DOI： 10.13422/j.cnki.syfjx.20210802.

摘要

目的

探讨黄连解毒汤对

-淀粉样蛋白（A

）

1-42

诱导阿尔茨海默病（AD）模型大鼠学习记忆能力和胆碱能系统的影响。

方法

选用60只雄性SD大鼠，分为正常组，模型组，石杉碱甲组（2.1×10

-5

g·kg

-1

），黄连解毒汤高、中、低剂量组（6，3，1.5 g·kg

-1

）。运用A

1-42

海马注射复制AD大鼠模型，术后连续灌胃治疗4周，进行Morris水迷宫实验。苏木素-伊红（HE）染色观察大鼠海马病理改变，腹主动脉取血，酶联免疫吸附测定（ELISA）检测大鼠血清、海马中乙酰胆碱（ACh），乙酰胆碱酯酶（AchE），胆碱乙酰转移酶（ChAT）含量；蛋白免疫印迹法（Western blot）检测海马

7烟碱型乙酰胆碱受体（

7nAChR）蛋白表达；实时荧光定量聚合酶链式反应法（Real-time PCR）检测海马

7nAChR mRNA表达。

结果

与正常组比较，模型组动物脑组织皮质区神经元坏死、海马区锥体细胞或颗粒细胞坏死、排列紊乱和炎性细胞浸润等病理改变明显，大鼠逃避潜伏期延长、逃逸平台次数降低、有效区域停留时间、有效区域游泳路径均减少（

0.05，

0.01），血清中ACh，ChAT浓度降低（

0.01），海马中ACh，AchE，ChAT含量，

7nAChR蛋白，

7nAChR mRNA表达降低（

0.01）；与模型组比较，黄连解毒汤中剂量组逃避潜伏期变短（

0.05），逃逸平台次数、有效区域游泳路径、有效区域停留时间增加（

0.05，

0.01），血清ACh，ChAT含量和海马AchE，ChAT，

7nAChR mRNA表达上升（

0.05），海马

7nAChR蛋白表达升高明显（

0.01）；高剂量组有效区域有效区域停留时间延长（

0.01）；逃逸平台次数增加，血清ACh，ChAT含量和海马ACh，AchE，

7nAChR蛋白，

7nAChR mRNA表达上升（

0.05）。

结论

黄连解毒汤可显著改善A

1-42

诱导AD大鼠学习记忆能力，其作用机制可能与改善A

1-42

所导致的胆碱能系统损伤，增强胆碱能系统功能有关。

Abstract

Objective

To investigate the effects of Huanglian Jiedutang on learning and memory ability and the cholinergic system in Alzheimer's disease（AD） rats induced by amyloid

-protein（A

）

1-42

Method

Sixty male SD rats were divided into normal group， model group， huperzine A group （2.1×10

-5

g·kg

-1

）， high-， medium- and low dose of Huanglian Jiedutang groups （6，3，1.5 g·kg

-1

）. AD rat model was replicated by hippocampal injection of A

1-42

. After 4 weeks of treatment， Morris water maze test was performed. Hematoxylineosin （HE） staining was used to observe the pathological changes of rat hippocampus. Sampling blood from abdominal aorta was taken. Acetylcholine （ACh）， acetylcholinesterase （AchE） and choline acetyltransferase （ChAT） in serum and hippocampus were detected by enzyme-linked immunosorbent assay （ELISA）. The expression of hippocampal

7 nicotinic acetylcholine receptor （

7nAChR） protein was detected by Western blot. The expression of hippocampal

7nAChR mRNA was detected by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）.

Result

Compared with the normal group， there were obvious pathological changes in the model group，such as neuron necrosis in the cerebral cortex，pyramidal cell or granular cell necrosis in the hippocampus，disorder of arrangement and inflammatory cell infiltration，prolonged escape latency，decreased escape platform times，decreased residence time in the effective area and swimming path in the effective area （

0.05，

0.01）. The contents of

7nAChR mRNA，ACh，AchE，ChAT，

7nAChR in the hippocampus decreased （

0.01）. Compared with the model group，the escape latency of the middle dose group was shorter （

0.05）， the escape platform times，the swimming path in the effective area and the residence time in the effective area increased （

0.05，

0.01）， the contents of serum ACh，ChAT， hippocampal AchE，ChAT and

7nAChR increased （

0.05，）. The expression of hippocampal

7nAChR protein significantly increased （

0.01）， the residence time of effective area in high dose group was prolonged （

0.01）， the times of escape platform increased，and the contents of serum ACh，ChAT and hippocampal ACh，AchE，

7nAChR protein and

7nAChR mRNA increased （

0.05）.

Conclusion

Huanglian Jiedutang can significantly improve the learning and memory ability of AD rats induced by A

1-42

，and its mechanism may be related to the improvement of cholinergic system damage and enhancement of cholinergic system function induced by A

1-42

关键词

Keywords

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Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by Aβ1-42

DOI：10.13422/j.cnki.syfjx.20210802

摘要

Abstract

关键词

Keywords

references

Effect of Huanglian Jiedutang on Learning and Memory Ability and Cholinergic System in AD Rats Induced by Aβ_1-42