Effect of Different Extracts of Thlaspi Herba on Gut Microbiota of Hyperuricemia Mice

Xi KANG; Hui-hui HAO; Cheng-lin MU; Tao-tao LU; Xue-lian WANG; Li-jing TANG; Rong-ping YANG

doi:10.13422/j.cnki.syfjx.20211249

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Effect of Different Extracts of Thlaspi Herba on Gut Microbiota of Hyperuricemia Mice

Drug Metabolism | 更新时间：2021-07-28

- Effect of Different Extracts of Thlaspi Herba on Gut Microbiota of Hyperuricemia Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 17, Pages: 132-138(2021)
- 作者机构：
  
  1.成都中医药大学药学院，成都 611137
  2.西南大学药学院，重庆 400715
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20211249
  CLC： R22;R378.2;R28;R696+6
- Received：24 December 2020，
  
  Published Online：25 March 2021，
  
  Published：05 September 2021
- 稿件说明：
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康希,郝慧汇,穆成林等.菥蓂不同提取物对高尿酸血症小鼠肠道菌群的影响[J].中国实验方剂学杂志,2021,27(17):132-138.

KANG Xi,HAO Hui-hui,MU Cheng-lin,et al.Effect of Different Extracts of Thlaspi Herba on Gut Microbiota of Hyperuricemia Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(17):132-138.
康希,郝慧汇,穆成林等.菥蓂不同提取物对高尿酸血症小鼠肠道菌群的影响[J].中国实验方剂学杂志,2021,27(17):132-138. DOI： 10.13422/j.cnki.syfjx.20211249.

KANG Xi,HAO Hui-hui,MU Cheng-lin,et al.Effect of Different Extracts of Thlaspi Herba on Gut Microbiota of Hyperuricemia Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(17):132-138. DOI： 10.13422/j.cnki.syfjx.20211249.

摘要

目的

探索菥蓂不同提取物对高尿酸血症小鼠肠道菌群的影响，以揭示其降尿酸活性物质基础和作用机制。

方法

将88只雄性昆明鼠小鼠分为11组，即空白组，模型组，别嘌呤醇组，石油醚提取物高、低剂量组，乙酸乙酯提取物高、低剂量组，正丁醇提取物高、低剂量组，总黄酮提取物高、低剂量组。空白组小鼠灌胃0.5%羧甲基纤维素钠，其他组小鼠均灌胃氧嗪酸钾（500 mg·kg

-1

）以复制高尿酸血症模型。造模若干小时后，空白组和模型组均灌胃蒸馏水，别嘌呤醇组给予别嘌呤醇混悬液（50 mg·kg

-1

）。各给药组按高、低剂量（5，2.5 g·kg

-1

）灌胃给药，每日1次，持续14 d后取血，测定血尿酸（SUA）水平和黄嘌呤氧化酶（XOD）活性，收集新鲜粪便进行16S rDNA测序。

结果

与空白组比较，模型组SUA水平和XOD活性明显升高（

0.05）；与模型组相比，别嘌呤醇组SUA水平和XOD活性显著下降（

0.01），菥蓂给药组干预后，除石油醚提取物高、低剂量组和总黄酮提取物低剂量组外，SUA水平均明显降低（

0.05，

0.01），石油醚提取物低剂量组、总黄酮提取物高剂量组和正丁醇提取物高、低剂量组可明显抑制XOD活性（

0.05，

0.01），其中总黄酮提取物高剂量组降低SUA水平和抑制XOD活性最显著。菌群测序结果显示，模型组

多样性和菌群丰度变化显著，且发现拟杆菌门（Bacteroidetes），厚壁菌门（Firmicutes）和乳酸杆菌科（Lactobacillaceae）与XOD活性显著相关；给药组干预后菥蓂总黄酮提取物高、低剂量组操作分类单元（OTU），ACE，Chao1，Shannon指数明显升高（

0.05，

0.01）；乙酸乙酯提取物低剂量组、总黄酮提取物高剂量组和正丁醇提取物高、低剂量组的拟杆菌门相对丰度显著降低（

0.01），厚壁菌门相对丰度显著升高（

0.01）；乳酸杆菌科相对丰度在正丁醇提取物低剂量组、总黄酮提取物高剂量组中显著升高（

0.01）。

结论

菥蓂降尿酸的有效部位主要为黄酮类成分，通过影响肠道菌群如乳酸杆菌、拟杆菌和厚壁菌进而改善机体SUA水平和XOD活性可能是其作用机制之一。

Abstract

Objective

To explore the effect of different extracts of Thlaspi Herba on the gut microbiota of hyperuricemia mice， and to reveal the substance basis and mechanism of its hypouricemic activity.

Method

Eighty-eight male Kunming mice were divided into 11 groups， including blank group， model group， allopurinol group， high and low dose groups of petroleum ether extract， high and low dose groups of ethyl acetate extract， high and low dose groups of

-butanol extract， high and low dose groups of total flavonoids extract. Mice in the blank group were given 0.5% sodium carboxymethylcellulose by gavage， and the other groups were given oteracil potassium （500 mg·kg

-1

） by gavage to duplicate the hyperuricemia model. After modeling for several hours， the blank group and the model group were given distilled water by gavage， while mice in the allopurinol group were given allopurinol suspension （50 mg·kg

-1

）， and mice in each treatment group were given high and low doses of corresponding extract （5， 2.5 g·kg

-1

）. The serum uric acid （SUA） level and xanthine oxidase （XOD） activity were measured after 14 days. Fresh feces were collected for 16S rDNA sequencing.

Result

Compared with the blank group， SUA level and XOD activity of model group were significantly increased （

0.05）. Compared with the model group， SUA level and XOD activity of the allopurinol group were significantly decreased （

0.01）. After intervention， SUA level were significantly decreased （

0.05，

0.01）， except for high dose and low dose groups of petroleum ether extract and low dose group of total flavonoids extract， XOD activity was significantly inhibited in low dose group of petroleum ether extract， high dose group of total flavonoids extract， and high and low dose groups of

-butanol extract （

0.05，

0.01）. The high dose group of total flavonoids extract was the most significant. The results of flora sequencing showed that

diversity and abundance of the model group changed significantly， and Bacteroidetes， Firmicutes and Lactobacillaceae were significantly correlated with XOD activity. After intervention， the operational taxonomic unit （OTU）， ACE， Chao1 and Shannon indexes of the high and low dose groups of total flavonoids extract were significantly increased （

0.05，

0.01）. Relative abundance of Bacteroidetes in low dose group of ethyl acetate extract， high dose group of total flavonoids extract， and high and low dose groups of

-butanol extract was significantly decreased （

0.01）， and the relative abundance of Firmicutes was significantly increased （

0.01）. The relative abundance of Lactobacillaceae in low dose group of

-butanol extract and high dose group of total flavonoids extract was significantly increased （

0.01）.

Conclusion

The effective part of Thlaspi Herba for reducing uric acid is mainly flavonoids， the improvement of SUA level and XOD activity by affecting gut microbiota such as Lactobacillaceae， Bacteroidetes and Firmicutes， may be one of its mechanisms.

关键词

Keywords

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