Objective

2

To carry out germplasm resource evaluation and new variety breeding of

Murraya paniculata

and improve the germplasm quality， so as to ensure the demand， yield and quality of medicinal materials.

Method

2

Following resource investigation and collection， 17 traits of 107

M. paniculata

germplasm samples， like plant type， basal diameter， leaf shape， leaf length， and leaf width were determined and then subjected to principal component analysis and factor analysis for screening the principal component factors. Nine primary traits were selected as variables for further cluster analysis using Ward's method and Euclidean distance. According to the characteristics of medicinal parts， the core germplasms were screened out. Then the contents of auxin， zeatin， zeatin nucleoside， isopentenyl adenine， isopentenyl adenine riboside， dihydrozeatin， and dihydrozeatinriboside in the leaves were measured by ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）， followed by their correlation analysis with agronomic trait.

Result

2

The variation coefficients of petiole length， branching number， and basal diameter were large. The nine main factors could be classified into four categories， with a contribution rate of 72.822%. The cluster analysis with Ward's method and Euclidean distance showed that 107 germplasm samples were clustered into six clusters and 61 core germplasms were identified. Such traits as leaf length， leaf width， petiole length， leaf surface， and petiole color were found to play an important role in the classification of

M. paniculata

germplasms. The content of zeatin nucleoside exhibited significant positive correlations with leaf length （

P

<

0.01）， petiole length （

P

<

0.01）， and leaf width （

P

<

0.05）.

Conclusion

2

These results have laid the foundation for further selection and breeding of

M. paniculata

new varieties.