Inhibitory Effect of Sangsuyin on Airway Inflammation in Asthmatic Rats by Regulating TLR4 Pathway

Jian-bin ZHAI; Liang ZHAO; Shi-chang YU; Chen-liang ZHAO; Hong-da ZHAO

doi:10.13422/j.cnki.syfjx.20211594

您当前的位置：

首页 >

文章列表页 >

Inhibitory Effect of Sangsuyin on Airway Inflammation in Asthmatic Rats by Regulating TLR4 Pathway

Classic Prescriptions | 更新时间：2021-06-25

- Inhibitory Effect of Sangsuyin on Airway Inflammation in Asthmatic Rats by Regulating TLR4 Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 14, Pages: 21-28(2021)
- 作者机构：
  
  河北省中医院，石家庄 050000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20211594
  CLC： R2-0;R22;R285.5;R256.12
- Received：24 February 2021，
  
  Published Online：02 June 2021，
  
  Published：20 July 2021
- 稿件说明：
移动端阅览
翟建宾,赵亮,于士昌等.桑苏饮调控TLR4通路抑制哮喘大鼠气道炎症[J].中国实验方剂学杂志,2021,27(14):21-28.

ZHAI Jian-bin,ZHAO Liang,YU Shi-chang,et al.Inhibitory Effect of Sangsuyin on Airway Inflammation in Asthmatic Rats by Regulating TLR4 Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(14):21-28.
翟建宾,赵亮,于士昌等.桑苏饮调控TLR4通路抑制哮喘大鼠气道炎症[J].中国实验方剂学杂志,2021,27(14):21-28. DOI： 10.13422/j.cnki.syfjx.20211594.

ZHAI Jian-bin,ZHAO Liang,YU Shi-chang,et al.Inhibitory Effect of Sangsuyin on Airway Inflammation in Asthmatic Rats by Regulating TLR4 Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(14):21-28. DOI： 10.13422/j.cnki.syfjx.20211594.

摘要

目的

探讨桑苏饮调控Toll样受体4（TLR4）通路对哮喘大鼠气道炎症的抑制作用。

方法

48只SD大鼠中选取40只建立哮喘模型，并按照随机数表法等分为模型组，地塞米松组，桑菊饮低、中、高剂量组5组，剩余8只SD大鼠作为空白组。地塞米松组予以0.005 g·kg

-1

灌胃，桑苏饮低、中、高剂量组分别予以2.1，4.2，8.4 g·kg

-1

桑苏饮灌胃（给药体积0.01 L·kg

-1

），模型组和空白组分别予以给药体积0.01 L·kg

-1

灌胃生理盐水，均每天1次，共7 d。比较干预后各组大鼠过敏反应分级；酶联免疫吸附测定法（ELISA）检测各组大鼠干预后血清，支气管肺泡灌洗液（BALF）白细胞介素-1

（IL-1

），肿瘤坏死因子-

（TNF-

），

γ-

干扰素（IFN-

）水平；苏木精-伊红（HE）染色观察各组肺组织病理改变，比较肺组织炎症细胞浸润评分；实时荧光定量聚合酶链式反应（Real-time PCR）检测各组肺组织TLR4，核转录因子-

B（NF-

B）mRNA表达；蛋白免疫印迹法（Western blot）检测各组肺组织TLR4，NF-

B蛋白表达及磷酸化（p）-NF-

B水平。

结果

模型组过敏反应分级、肺组织病理改变较空白组加重，其余各组较模型组均有所减轻；模型组血清，BALF IL-1

，TNF-

水平均高于空白组，其余各组均低于模型组（

0.01）；模型组血清，BALF IFN-

水平均低于空白组，其余各组均高于模型组（

0.05，

0.01）；模型组肺组织炎症细胞浸润评分，肺组织TLR4，NF-

B mRNA与蛋白表达，p-NF-

B蛋白水平均高于空白组，其余各组均低于模型组（

0.01），差异有统计学意义。

结论

桑苏饮可抑制哮喘大鼠过敏反应、肺组织病变和气道炎症，可能是通过抑制TLR4通路，下调TLR4，NF-

B mRNA与蛋白表达，降低p-NF-

B水平实现。

Abstract

Objective

To investigate the inhibitory effect of Sangsuyin on airway inflammation in asthmatic rats by regulating the Toll-like receptor 4 （TLR4） pathway.

Method

Forty-eight SD rats were randomly divided into a normal group （

=8） and an experimental group （

=40）. Asthma model was induced in the rats of the experimental group which were further divided into the following five groups according to a random number table： a model group， a dexamethasone group （0.005 g·kg

-1

），and low- （2.1 g·kg

-1

）， medium- （4.2 g·kg

-1

）， and high-dose （8.4 g·kg

-1

） Sangsuyin groups. The drugs were all dissolved in normal saline at 0.01 L·kg

-1

. The rats in the model group and the normal group received normal saline （

） at 0.01 L·kg

-1

. The drug treatment was carried out once per day， for a total of 7 days. The grades of allergic reactions were compared among the groups after intervention. The levels of interleukin-1

（IL-1

），tumor necrosis factor-

（TNF-

）， and interferon-

（IFN-

） in serum and bronchoalveolar lavage fluid （BALF） were detected by enzyme-linked immunosorbent assay （ELISA）. Hematoxylin-eosin （HE） staining was used to observe the pathological changes of lung tissues，and the inflammatory cell infiltration scores of lung tissues were compared. The mRNA expression of TLR4 and nuclear factor-

B （NF-

B） was detected by quantitative real-time fluorescence-based reverse-transcription polymerase chain reaction （Real-time PCR）. The expression of TLR4，NF-

B， and the level of phosphorylated NF-

B （p-NF-

B） were detected by Western blot.

Result

The success rate of modeling was 95.00%. Compared with the normal group， the model group showed a higher grade of allergic reaction and more severe pathological changes of lung tissues，and the groups with drug intervention exhibited relieved conditions. The levels of IL-1

and TNF-

in the serum and BALF were higher in the model group than in the normal group，and lower in the groups with drug intervention than in the model group （

0.01）. The level of IFN-

in the serum and BALF was lower in the model group than in the normal group，and higher in the groups with drug intervention than in the model group （

0.05，

0.01）. The inflammatory cell infiltration score，mRNA and protein expression of TLR4 and NF-

B，and the mRNA level of p-NF-

B were higher in the model group than in the normal group，and lower in the groups with drug intervention than in the model group （

0.01）.

Conclusion

Sangsuyin could inhibit allergic reactions，lung tissue lesions， and airway inflammation in asthmatic rats. It is speculated that this effect is achieved by inhibiting the TLR4 pathway，down-regulating the mRNA and protein expression of TLR4 and NF-

B，and reducing the level of p-NF-

B.angsuyin can inhibit allergic reaction，lung tissue lesions and airway inflammation in asthmatic rats. It is speculated that this effect is achieved by inhibiting TLR4 pathway，down regulating the mRNA and protein expression of TLR4，NF-

B，and reducing the levels of p-NF-

关键词

Keywords

references

中华医学会变态反应分会呼吸过敏学组，中华医学会呼吸病学分会哮喘学组 . 中国过敏性哮喘诊治指南（第一版，2019年）［J］. 中华内科杂志， 2019 ， 58 （ 9 ）： 636 - 655 .

ABAYA R ， JONES L ， ZORC J J . Dexamethasone compared to prednisone for the treatment of children with acute asthma exacerbations ［J］. Pediatr Emerg Care ， 2018 ， 34 （ 1 ）： 53 - 58 .

苏奎国，姜良铎，郭勇英，等 . 桑苏饮对哮喘模型大鼠气道重塑及肺组织上皮细胞ICAM-1表达的影响［J］. 山东中医药大学学报， 2010 ， 34 （ 4 ）： 354 - 356 .

WANG Z R ， WANG Q ， SUI Y ， et al . Dexamethasone alleviates allergic asthma immature rat through Toll like receptor 4 ［J］. Eur Rev Med Pharmacol Sci ， 2018 ， 22 （ 1 ）： 184 - 189 .

JIAO W ， CUI H ， HUO Y ， et al . Effects of Baicalin on the activation of Toll like receptor 4/NF- κ B signaling pathway in mice with recurrent abortion ［J］. World Chinese Medicine ， 2020 ， 15 （ 6 ）： 846 - 849 .

MA S Q ， WEI H L ， ZHANG X . TLR2 regulates allergic airway inflammation through NF- κ B and MAPK signaling pathways in asthmatic mice ［J］. Eur Rev Med Pharmacol Sci ， 2018 ， 22 （ 10 ）： 3138 - 3146 .

国家药典委员会 . 中华人民共和国药典：一部［M］. 北京. 中国医药科技出版社， 2020 .

杨飞，惠毅，闫曙光，等 . 卵蛋白加氢氧化铝致敏建立哮喘大鼠模型研究［J］. 现代中西医结合杂志， 2018 ， 27 （ 15 ）： 1600 - 1602 .

SCHOETTLER N ， STREK M E . Recent advances in severe asthma：from phenotypes to personalized medicine ［J］. Chest ， 2020 ， 157 （ 3 ）： 516 - 528 .

LOVINSKY-DESIR S . The use of biologic therapies for the management of pediatric asthma ［J］. Pediatr Pulmonol ， 2020 ， 55 （ 3 ）： 803 - 808 .

袁为远，魏盼，包凯帆，等 . 麻黄-甘草药对抑制过敏性哮喘的效应及机制初探［J］. 南京中医药大学学报， 2020 ， 36 （ 1 ）： 41 - 45 .

高琴琴，丁子桐，李友林，等 . 不同剂量卵蛋白诱发BALB/c小鼠支气管哮喘模型的比较［J］. 中国比较医学杂志， 2019 ， 29 （ 4 ）： 52 - 57 .

陈秋仪，任培中，王佳美，等 . 加减乌梅丸颗粒对哮喘大鼠激素干预模型气道重塑及转化生长因子- β 1 /Smad信号通路的影响［J］. 世界中医药， 2020 ， 15 （ 8 ）： 1114 - 1119 .

陈叶，黄丽娟，胡智立，等 . 鱼腥草素钠对急性哮喘模型小鼠炎症的影响及机制研究［J］. 药物评价研究， 2019 ， 42 （ 6 ）： 70 - 74 .

MAHMUTOVIC-PERSSON I ， MENZEL M ， RAMU S ， et al . IL-1 β mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation ［J］. Respir Res ， 2018 ， 19 （ 1 ）： 16 .

GHEBRE M A ， PANG P H ， DIVER S ， et al . Biological exacerbation clusters demonstrate asthma and chronic obstructive pulmonary disease overlap with distinct mediator and microbiome profiles ［J］. J Allergy Clin Immunol ， 2018 ， 141 （ 6 ）： 2027 - 2036 .

SHARMA N ， AKKOYUNLU M ， RABIN R L . Macrophages-common culprit in obesity and asthma ［J］. Allergy ， 2018 ， 73 （ 6 ）： 1196 - 1205 .

SCHURMAN S H ， BRAVO M A ， INNES C L ， et al . Toll-like receptor 4 pathway polymorphisms interact with pollution to influence asthma diagnosis and severity ［J］. Sci Rep ， 2018 ， 8 （ 1 ）： 12713 .

王亚洲，何鹏，王丹虹 . 孟鲁司特钠通过抑制TLR4/NF- κ B信号通路影响哮喘大鼠气道平滑肌细胞增殖和凋亡［J］. 吉林大学学报：医学版， 2020 ， 46 （ 2 ）： 77 - 82 .

韩超，杨柳，张秋玲，等 . 黄芩苷抗哮喘作用与HMGB1/TLR4传导通路的相关性研究［J］. 世界中医药， 2019 ， 14 （ 6 ）： 1402 - 1407 .

韩超，杨柳，张秋玲，等 . 黄芩苷对哮喘大鼠气道重塑作用的实验研究［J］. 中国临床药理学与治疗学， 2017 ， 22 （ 7 ）： 749 - 754 .

吴慧芬 . 鲜鱼腥草挥发油对哮喘豚鼠肺组织干细胞因子和KL-6的影响［J］. 儿科药学杂志， 2017 ， 14 （ 6 ）： 5 - 7 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role of PPARγ in Ulcerative Colitis and Traditional Chinese Medicine Intervention： A Review

Yijingtang Reduces Ovarian Inflammatory Responses in Rat Model of Diminished Ovarian Reserve via TLR4/MyD88/NF-κB Signaling Pathway

Modified Guishenwan Alleviates Inflammation in Rat Model of Polycystic Ovary Syndrome by Inhibiting MAPK/NF-κB Pathway

Traditional Chinese Medicine Treats Tumors via MAPK/NF-κB Signaling Pathway： A Review

Related Author

ZHANG Wei

ZOU Menglong

XU Yin

ZHU Ying

HU Heng

CHU Jijun

LI Zhe

CHU Haijing

Related Institution

The First Clinical College of Traditional Chinese Medicine，Hunan University of Chinese Medicine

The First Hospital of Hunan University of Chinese Medicine

The First Clinical Medical School of Anhui University of Chinese Medicine

The First Affiliated Hospital of Anhui University of Chinese Medicine

The First Affiliated Hospital of Chongqing Medical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰