Effect of Fangji Huangqitang on DBA/1 Mice Collagen Induced Arthritis and Synovial Angiogenesis

Lian-hua HE; Hui-jie LUAN; Hong-ying SHAN; Bo YANG; Juan HE; Yi-ping HU; Jia-jing DAI; Hui-juan WAN; Chong-zhou FANG; Qing-wen WANG

doi:10.13422/j.cnki.syfjx.20211736

您当前的位置：

首页 >

文章列表页 >

Effect of Fangji Huangqitang on DBA/1 Mice Collagen Induced Arthritis and Synovial Angiogenesis

Classic Prescriptions | 更新时间：2021-07-28

- Effect of Fangji Huangqitang on DBA/1 Mice Collagen Induced Arthritis and Synovial Angiogenesis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 17, Pages: 16-23(2021)
- 作者机构：
  
  1.北京大学深圳医院，广东深圳 518036
  2.深圳市炎症与免疫性疾病重点实验室，广东深圳 518036
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20211736
  CLC： R2-0;R289;R33;R364.5
- Received：25 May 2021，
  
  Published Online：07 July 2021，
  
  Published：05 September 2021
- 稿件说明：
移动端阅览
何莲花,栾慧杰,单宏颖等.防己黄芪汤对DBA/1小鼠胶原诱导型关节炎及血管新生的影响[J].中国实验方剂学杂志,2021,27(17):16-23.

HE Lian-hua,LUAN Hui-jie,SHAN Hong-ying,et al.Effect of Fangji Huangqitang on DBA/1 Mice Collagen Induced Arthritis and Synovial Angiogenesis[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(17):16-23.
何莲花,栾慧杰,单宏颖等.防己黄芪汤对DBA/1小鼠胶原诱导型关节炎及血管新生的影响[J].中国实验方剂学杂志,2021,27(17):16-23. DOI： 10.13422/j.cnki.syfjx.20211736.

HE Lian-hua,LUAN Hui-jie,SHAN Hong-ying,et al.Effect of Fangji Huangqitang on DBA/1 Mice Collagen Induced Arthritis and Synovial Angiogenesis[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(17):16-23. DOI： 10.13422/j.cnki.syfjx.20211736.

摘要

目的

观察防己黄芪汤对DBA/1小鼠胶原诱导型关节炎（CIA）及血管新生的影响。

方法

将DBA/1小鼠按体质量随机分为正常组，CIA组及防己黄芪汤组（5.4 g·kg

-1

）。CIA组及防己黄芪汤组第1天以牛Ⅱ型胶原和完全弗氏佐剂免疫DBA/1小鼠，于第21天以牛Ⅱ型胶原和不完全弗氏佐剂免疫DBA/1小鼠建立CIA模型，并于二次免疫当天开始灌胃给药，每天1次，共给药28 d。第22天开始观察CIA小鼠的关节红肿等症状并进行关节炎评分，第49天取材后，进行组织苏木素-伊红（HE）染色观察CIA小鼠关节滑膜中血管新生情况；免疫组化检测CIA小鼠关节滑膜中血管内皮细胞标志物血小板内皮细胞黏附分子-1（CD31）及血管内皮生长因子（VEGF）的表达情况；免疫荧光双染检测CIA小鼠关节滑膜中成熟血管和不成熟血管的情况。同时，提取大鼠胸主动脉环，采用VEGF（20 μg·L

-1

）诱导大鼠胸主动脉环微血管的生长，加入不同浓度防己黄芪汤（0.25，0.5，1 g·L

-1

）作用后，显微镜下观察防己黄芪汤对VEGF诱导的大鼠胸主动脉环微血管生长情况并进行拍照统计。

结果

与正常组比较，CIA组小鼠炎症关节红、肿及畸形明显，临床关节炎评分、发病率、关节滑膜炎症、血管新生情况显著升高，关节滑膜中的血管密度，CD31和VEGF的阳性表达量、不成熟血管的数量均显著升高（

0.01）；与CIA组比较，防己黄芪汤组小鼠炎症关节的红、肿及畸形明显改善，临床关节炎评分、发病率、关节滑膜炎症、血管新生情况显著降低，关节滑膜中的血管密度，CD31和VEGF的阳性表达量、不成熟血管的数量均显著下降（

0.01）。与正常组比较，VEGF能显著诱导大鼠胸主动脉环微血管的生长（

0.01）；与VEGF组比较，防己黄芪汤（0.25，0.5，1 g·L

-1

）可显著抑制大鼠胸主动脉环微血管的生成（

0.01）。

结论

防己黄芪汤可有效缓解CIA小鼠的临床症状，降低临床关节炎评分及发病率，同时具有抑制CIA小鼠关节滑膜中及大鼠胸主动脉环中血管新生的作用。

Abstract

Objective

To observe the effect of Fangji Huangqitang （FJHQT） on collagen induced arthritis （CIA） and synovial angiogenesis in DBA/1 mice.

Method

DBA/1 mice were randomly divided into normal group， CIA group and FJHQT group. DBA/1 mice in CIA group and FJHQT group were immunized with bovine type Ⅱ collagen and complete Freund's adjuvant on the first day， and DBA/1 mice were immunized with bovine type Ⅱ collagen and incomplete Freund's adjuvant on the 21

day to establish CIA model. On the day of the second immunization， the drug was given by gavage once a day for 28 days. On the 22

day， the arthritis score and other symptoms of CIA mice were observed. On the 49

day， Hematoxylin eosin （HE） staining was carried out to observe the angiogenesis in the synovium of CIA mice， the expression of vascular endothelial cell marker platelet endothelial cell adhesion molecule-1 （CD31） and vascular endothelial growth factor （VEGF） in the synovium of CIA mice were detected. Immunofluorescence double staining was used to detect the mature and immature vessels in the synovium of CIA mice. And the microvascular growth of the rat thoracic aortic ring was induced by VEGF （20 μg·L

-1

）. The effects of FJHQT （0.25， 0.5， 1 g·L

-1

） at different concentrations were observed under microscope.

Result

Compared with the normal group， the inflammation， joints， red and swelling of the inflammatory joints of the CIA group were significantly increased （

0.01）. The scores of clinical arthritis， the incidence rate， synovial inflammation and angiogenesis were significantly increased （

0.01）. The density of blood vessels， the positive expression of CD31 and VEGF， the number of immature vessels in synovial membrane were significantly increased （

0.01）. And compared with the CIA group， the inflammation， joint swelling， and malformation of the FJHQT group were significantly improved， the clinical arthritis score， incidence rate， synovial inflammation and angiogenesis were significantly reduced （

0.01）. The vascular density， the positive expression of CD31 and VEGF， and the number of immature blood vessels in synovial membrane were significantly increased （

0.01）. Compared with blank group， VEGF could significantly induce the growth of microvasculature in rat thoracic aortic ring （

0.01）. Compared with VEGF group， FJHQT（0.25， 0.5， 1 g·L

-1

） could significantly inhibit the formation of microvasculature in rat thoracic aortic ring （

0.01）.

Conclusion

FJHQT can effectively alleviate the clinical symptoms and condition of CIA mice， reduce the clinical arthritis score and incidence rate，and inhibit the synovial angiogenesis of CIA mice joints and VEGF induced microvascular formation in rat thoracic aortic rings.

关键词

Keywords

references

SMOLEN J S ， ALETAHA D ， MCINNES I B . Rheumatoid arthritis ［J］. Lancet ， 2016 ， 388 （ 10055 ）： 2023 - 2038 .

SMOLEN J S ， ALETAHA D ， BARTON A ， et al . Rheumatoid arthritis ［J］. Nat Rev Dis Primers ， 2018 ， 4 ： 18001 .

贾庆运，王拥军，梁倩倩，等 . 防己黄芪汤对胶原诱导性关节炎小鼠的影响［J］. 中国中医骨伤科杂志， 2018 ， 26 （ 5 ）： 6 - 9 .

HE L H ， QIN Q X ， HE J ， et al . ErMiao San inhibits angiogenesis in rheumatoid arthritis by suppressing JAK/STAT signaling pathways ［J］. Evid Based Complement Alternat Med ， 2020 ， doi： 10.1155/2020/4381212 http://dx.doi.org/10.1155/2020/4381212 .

刘春芳，王靖霞，何莲花，等 . 风湿祛痛胶囊对Ⅱ型胶原诱导性关节炎大鼠滑膜血管新生的影响［J］. 中国中药杂志， 2019 ， 44 （ 7 ）： 1457 - 1463 .

王靖霞，刘春芳，何莲花，等 . 风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响［J］. 中国实验方剂学杂志， 2019 ， 25 （ 2 ）： 89 - 95 .

HE L H ， LIU C F ， SUN C C ， et al . Wu-Tou Decoction inhibits angiogenesis in experimental arthritis by targeting VEGFR2 signaling pathway ［J］. Rejuvenation Res ， 2018 ， 21 （ 5 ）： 442 - 455 .

KOCH A E . Angiogenesis：implications for rheumatoid arthritis ［J］. Arthritis Rheum ， 1998 ， 41 （ 6 ）： 951 - 962 .

PALEOLOG E M . Angiogenesis in rheumatoid arthritis ［J］. Arthritis Res ， 2002 ， 4 （ 3 ）： 81 - 90 .

IZQUIERDO E ， CANETE J D ， CELIS R ， et al . Immature blood vessels in rheumatoid synovium are selectively depleted in response to anti-TNF therapy ［J］. PLoS One ， 2009 ， 4 （ 12 ）： e8131 .

TⅡDUS P M ， HOUSTON M E . Vitamin E status does not affect the responses to exercise training and acute exercise in female rats ［J］. J Nutr ， 1993 ， 123 ： 834 - 840 .

Al-SALAHI O S A ， KIT-LAM C ， MAJID A M S A ， et al . Anti-angiogenic quassinoid-rich fraction from Eurycoma longifolia modulates endothelial cell function ［J］. Microvasc Res ， 2013 ， 90 ： 30 - 39 .

崔昕 . 防己黄芪汤对类风湿性关节炎的疗效［J］. 国外医学：中医中药分册， 1996 ， 18 （ 5 ）： 569 - 574 .

陈桂香 . 防己黄芪汤合独活寄生汤加减治疗类风湿性关节炎32例疗效观察［J］. 中医药导报， 2012 ， 18 （ 4 ）： 58 - 59 .

张爱民 . 防己黄芪汤合独活寄生汤加减治疗类风湿性关节炎疗效观察［J］. 内蒙古中医药， 2017 ， 36 （ 3 ）： 19 .

万里伟，王燕君，兰凌凌，等 . 防己黄芪汤合独活寄生汤治疗类风湿性关节炎临床观察［J］. 中国中医药现代远程教育， 2020 ， 18 （ 3 ）： 71 - 72 .

曾维铨 . 防己黄芪汤合防风补骨方治疗膝关节骨性关节炎35例［J］. 现代中医药， 2016 ， 36 （ 2 ）： 31 - 32 .

陈月 . 防己黄芪汤合雷公藤片治疗类风湿性关节炎的疗效观察［J］. 四川中医， 2008 ， doi： 10.3969/j.issn.1000-3649.2008.01.045 http://dx.doi.org/10.3969/j.issn.1000-3649.2008.01.045 .

华英 . 防己黄芪汤合四物汤治疗老年性类风湿性关节炎的体会［J］. 浙江中医杂志， 2015 ， 50 （ 4 ）： 300 .

王绪辉，周重建，闵熙敬，等 . 防己黄芪汤提取物治疗活动期类风湿性关节炎的临床药效评估［J］. 中医杂志， 1993 ， 3 ： 156 - 158 .

杨晓凌，刘欢，陈亮，等 . 防己黄芪汤与非布司他对脾虚湿阻型痛风性关节炎的疗效［J］. 中国继续医学教育， 2018 ， 10 （ 25 ）： 142 - 144 .

史晓，陈建军 . 风1号合防己黄芪汤治疗类风湿性关节炎46例［J］. 南京中医药大学学报， 1998 ， 1 ： 55 .

GUO Y ， FAN Y ， PEI X . Fangjihuangqi decoction inhibits MDA‐MB‐231 cell invasion in vitro and decreases tumor growth and metastasis in triple‐negative breast cancer xenografts tumor zebrafish model ［J］. Cancer Med ， 2020 ， 9 （ 7 ）： 2564 - 2578 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Compatibility of Effective Monomer Components of Fujin Shengjisan on Angiogenesis of HUVEC Based on Uniform Design

Mechanism of Chinese Medicine in Promoting Angiogenesis After Ischemic Stroke Based on PI3K/Akt Signaling Pathway： A Review

Effect and Mechanism of Sishenjian on Synovial Lesions in Knee Osteoarthritis Rats

Anti-inflammation and Regulation of HIF-1α/VEGF of 3 Kinds of Borneolum in Prevention and Treatment of AMI Model Rats

Shexiang Baoxin Pills in Regulation of Angiogenesis in Coronary Heart Disease： A Review

Related Author

LU Xianying

GAO Jing

BAI Dingxi

HOU Chaoming

JI Wenting

CHEN Huan

WU Chenxi

WANG Xiaoya

Related Institution

School of Nursing， Chengdu University of Traditional Chinese Medicine

The First Clinical School of Henan University of Chinese Medicine

The First Affiliated Hospital of Henan University of Chinese Medicine

Guangdong Provincial Key Laboratory of Research and Development in TCM

Guangdong Provincial Second Hospital of Traditional Chinese Medicine（TCM）， Guangdong Provincial Engineering Technology Research Institute of TCM，The Fifth Clinical College of Guangzhou University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰