Comparison of UPLC Fingerprint and Determination of Main Components Before and After Processing of Artemisiae Argyi Folium Processed with Four Excipients

Li-xia WANG; Cong LIU; Xiao-yun YANG; Xiao-xu CHEN; Tong JIANG; Li-ying TANG; Zhu-ju WANG

doi:10.13422/j.cnki.syfjx.20211759

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Comparison of UPLC Fingerprint and Determination of Main Components Before and After Processing of Artemisiae Argyi Folium Processed with Four Excipients

更新时间：2021-10-21

- Comparison of UPLC Fingerprint and Determination of Main Components Before and After Processing of Artemisiae Argyi Folium Processed with Four Excipients
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 22, Pages: 147-154(2021)
- 作者机构：
  
  1.中国中医科学院中药研究所，北京 100700
  2.山西医科大学药学院，太原 030001
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20211759
  CLC： R22;R28;R943.1;O657.7
- Received：08 May 2021，
  
  Published Online：07 June 2021，
  
  Published：20 November 2021
- 稿件说明：
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王丽霞,刘聪,杨晓芸等.四制艾叶炮制前后的UPLC指纹图谱及主要成分含量比较[J].中国实验方剂学杂志,2021,27(22):147-154.

WANG Li-xia,LIU Cong,YANG Xiao-yun,et al.Comparison of UPLC Fingerprint and Determination of Main Components Before and After Processing of Artemisiae Argyi Folium Processed with Four Excipients[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(22):147-154.
王丽霞,刘聪,杨晓芸等.四制艾叶炮制前后的UPLC指纹图谱及主要成分含量比较[J].中国实验方剂学杂志,2021,27(22):147-154. DOI： 10.13422/j.cnki.syfjx.20211759.

WANG Li-xia,LIU Cong,YANG Xiao-yun,et al.Comparison of UPLC Fingerprint and Determination of Main Components Before and After Processing of Artemisiae Argyi Folium Processed with Four Excipients[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(22):147-154. DOI： 10.13422/j.cnki.syfjx.20211759.

摘要

目的

建立艾叶及四制艾叶的超高效液相色谱法（UPLC）指纹图谱，并对其所含的8种酚酸及黄酮类成分进行定量分析，探索四制艾叶质量评价方法。

方法

采用UPLC，Shim-pack XR-ODS C

色谱柱（2.0 mm×75 mm，2.2 µm），流动相乙腈（A）-0.2 %甲酸水溶液（B）梯度洗脱（0~1 min，10%A；1~2 min，10%~15%A；2~17 min，15%~18%A；17~24 min，18%~28%A；24~36 min，28%~38%A；36~41 min，38%~60%A；41~45 min，60%~100%A），检测波长330 nm，流速0.2 mL·min

-1

，建立艾叶、四制艾叶的UPLC指纹图谱，通过化学计量学方法对艾叶炮制前后指纹图谱进行分析，并对新绿原酸、绿原酸、隐绿原酸、棕矢车菊素、异泽兰黄素和异绿原酸A~C进行含量测定。

结果

建立了艾叶及四制艾叶的指纹图谱，艾叶及四制艾叶UPLC指纹图谱一致性较好，相似度均

0.94。与艾叶比较，四制后绿原酸和异绿原酸B的含量无明显变化，棕矢车菊素和异泽兰黄素含量下降；而新绿原酸、隐绿原酸和异绿原酸C含量显著升高（

0.01），平均升高率分别达到32.50%，66.83%和29.39%；异绿原酸A含量显著降低（

0.01），平均降低率达51.25%。

结论

艾叶与四制艾叶的化学成分含量发生了一定程度改变，其中新绿原酸、隐绿原酸、异绿原酸A和异绿原酸C可作为艾叶四制前后质量评价的关键性指标，可为艾叶和四制艾叶质量的深入研究及质量标准的建立提供科学依据。

Abstract

Objective

To establish the ultra-performance liquid chromatography （UPLC） fingerprints of Artemisiae Argyi Folium and Artemisiae Argyi Folium processed with four excipients， and quantitatively analyze the 8 phenolic acids and flavonoids contained in them， in order to explore the quality evaluation method of Artemisiae Argyi Folium processed with four excipients.

Method

UPLC was used with Shim-pack XR-ODS C

column （2.0 mm×75 mm， 2.2 µm）， mobile phase of acetonitrile （A） -0.2% formic acid aqueous solution （B） for gradient elution （0-1 min， 10%A； 1-2 min， 10%-15%A； 2-17 min， 15%-18%A； 17-24 min， 18%-28%A； 24-36 min， 28%-38%A； 36-41 min， 38%-60%A； 41-45 min， 60%-100%A）， detection wavelength of 330 nm and flow rate of 0.2 mL·min

-1

. The UPLC fingerprints of Artemisiae Argyi Folium before and after processing were established， and analyzed by chemometrics. Contents of 5-caffeoylquinic acid， 3-caffeoylquinic acid， 4-caffeoylquinic acid， 3，4-dicaffeoylquinic acid， 3，5-dicaffeoylquinic acid， 4，5-dicaffeoylquinic acid， jaceosidin and epuatilin in the decoction pieces were determined.

Result

The fingerprints of Artemisiae Argyi Folium before and after processing were established， and the UPLC characteristic chromatograms of Artemisiae Argyi Folium before and after processing had good consistency， and the similarity was

0.94. Compared with Artemisiae Argyi Folium， the contents of 3-caffeoylquinic acid and 3，4-dicaffeoylquinic acid had no significant change after processing， the contents of jaceosidin and epuatilin decreased after processing， while the contents of 5-caffeoylquinic acid， 4-caffeoylquinic acid and 4，5-dicaffeoylquinic acid increased significantly （

0.01）， their average increasing rates were 32.50%， 66.83%， 29.39%， respectively. And content of 3，5-dicaffeoylquinic acid was significantly decreased （

0.01）， and the average reduction rate was 51.25%.

Conclusion

The contents of chemical components in Artemisiae Argyi Folium and Artemisiae Argyi Folium processed with four excipients have changed to a certain extent. Among them， 5-caffeoylquinic acid， 3，5-dicaffeoylquinic acid， 4-caffeoylquinic acid and 4，5-dicaffeoylquinic acid can be used as the key indicators for quality evaluation of Artemisiae Argyi Folium before and after processing.

关键词

Keywords

references

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