Effect of Bushen Shugan Prescription on Oxidative Stress and TGF-<italic style="font-style: italic">β</italic><sub>1</sub>/Smad Signaling Pathway in Hippocampus of Senile Depressed Mice

Zhi-jing ZHANG; Jian-ping YAO; Qiang WEI; Zi-yi GUO; Hui-xian JIE; Di LI

doi:10.13422/j.cnki.syfjx.20212002

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Effect of Bushen Shugan Prescription on Oxidative Stress and TGF-β₁/Smad Signaling Pathway in Hippocampus of Senile Depressed Mice

更新时间：2021-10-14

- Effect of Bushen Shugan Prescription on Oxidative Stress and TGF-β₁/Smad Signaling Pathway in Hippocampus of Senile Depressed Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 21, Pages: 114-120(2021)
- 作者机构：
  
  河南中医药大学中医学院，郑州 450000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20212002
  CLC： R2-0;R22;R285.5;R289;R33
- Received：07 July 2021，
  
  Published Online：16 September 2021，
  
  Published：05 November 2021
- 稿件说明：
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张芷菁,姚建平,卫强等.补肾疏肝方对老年抑郁症小鼠海马氧化应激及TGF-β₁/Smad信号通路的影响[J].中国实验方剂学杂志,2021,27(21):114-120.

ZHANG Zhi-jing,YAO Jian-ping,WEI Qiang,et al.Effect of Bushen Shugan Prescription on Oxidative Stress and TGF-β₁/Smad Signaling Pathway in Hippocampus of Senile Depressed Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(21):114-120.
张芷菁,姚建平,卫强等.补肾疏肝方对老年抑郁症小鼠海马氧化应激及TGF-β₁/Smad信号通路的影响[J].中国实验方剂学杂志,2021,27(21):114-120. DOI： 10.13422/j.cnki.syfjx.20212002.

ZHANG Zhi-jing,YAO Jian-ping,WEI Qiang,et al.Effect of Bushen Shugan Prescription on Oxidative Stress and TGF-β₁/Smad Signaling Pathway in Hippocampus of Senile Depressed Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(21):114-120. DOI： 10.13422/j.cnki.syfjx.20212002.

摘要

目的

观察慢性轻度不可预见性应激（CUMS）后老年抑郁症小鼠海马组织氧化应激和转化生长因子-

（TGF-

）/Smad信号通路变化，并探讨补肾疏肝方抗抑郁的可能机制。

方法

5月龄的小鼠90只，随机分为正常组、模型组、补肾疏肝方高、中、低剂量组和氟西汀组共6组，每组15只。除正常组外，其余各组小鼠均采用CUMS建立老年抑郁症模型。补肾疏肝方高、中、低剂量组小鼠在造模第1天开始同时灌胃补肾疏肝方19.5，9.75，4.87 g·kg

-1

，氟西汀组小鼠灌胃氟西汀0.033 g·kg

-1

，正常组和模型组小鼠灌胃等体积生理盐水，共21 d。用矿场实验评价各组小鼠的行为反应，采集小鼠海马组织并进行相关指标检测，WST-1法测超氧化物歧化酶（SOD）含量，TBA法测丙二醛（MDA）的含量，微量酶标法测谷胱甘肽（GSH）含量，实时荧光定量聚合酶链式反应（Real-time PCR）检测TGF-

，Smad2，Smad3，Smad7 mRNA表达。

结果

与正常组比较，模型组小鼠OFT水平得分和垂直得分明显降低，海马组织SOD，GSH水平均下降，MDA含量明显升高（

0.05）；TGF-

，Smad2，Smad3 mRNA表达均上调，Smad7 mRNA表达下调（

0.05）。与模型组比较，补肾疏肝方高、中、低剂量组及氟西汀组小鼠海马组织SOD，GSH含量均上升，MDA含量下降（

0.05），补肾疏肝方高、中、低剂量组及氟西汀组小鼠海马组织TGF-

，Smad2，Smad3 mRNA表达均下调，Smad7 mRNA表达上调（

0.05）。与补肾疏肝方高剂量组比较，补肾疏肝方中、低剂量组小鼠海马组织SOD，GSH含量下降，MDA含量均上升（

0.05）；补肾疏肝方中、低剂量组小鼠海马组织TGF-

，Smad2，Smad3 mRNA表达均上调，Smad7 mRNA表达下调（

0.05）。

结论

补肾疏肝方可显著改善SAPM8小鼠的老年抑郁症状，其机制可能与调节海马氧化应激及TGF-

/Smad信号通路有关。

Abstract

Objective

To observe the changes in oxidative stress and transforming growth factor-

（TGF-

）/Smad signaling pathway in hippocampal tissue of senile depressed mice after chronic unpredictable mild stress and to explore the possible anti-depression mechanism of Bushen Shugan prescription.

Method

Ninety five-month-old mice were randomly divided into six groups， namely the normal group， senile depression model group， high-， medium-， and low-dose Bushen Shugan prescription groups， and fluoxetine group， with 15 in each group. Mice in all groups， except for the normal group， were exposed to chronic unpredictable mild stress （CUMS） for inducing the senile depression. Since the first day of modeling， the mice in the high-， medium- and low-dose Bushen Shugan prescription groups were gavaged with 19.5， 9.75， 4.87 g·kg

-1

Bushen Shugan prescription， the ones in the fluoxetine group with 0.033 g·kg

-1

fluoxetine， and those in the normal and senile depression model groups with an equal volume of normal saline for 21 successive days. The behavioral responses of mice in each group were evaluated in the open field test （OFT）， and the hippocampal tissues of mice were collected for testing the relevant indexes. The superoxide dismutase （SOD） content was determined by WST-1 method， malondialdehyde （MDA） content by TBA method， glutathione （GSH） content by micro enzyme-linked immunosorbent assay （ELISA）， and mRNA expression of TGF-

， Smad2， Smad3， and Smad7 by Real-time polymerase chain reaction （Real-time PCR）.

Result

Compared with the normal group， the senile depression model group exhibited significantly lowered horizontal and vertical scores in OFT， decreased SOD and GSH contents in hippocampal tissues， elevated MDA （

0.05）， up-regulated TGF-

， Smad2， and Smad3 mRNA expression， and down-regulated Smad7 （

0.05）. Compared with the senile depression model group， Bushen Shugan prescription at the high， medium， and low doses and fluoxetine all increased SOD and GSH contents in mouse hippocampal tissues， decreased the MDA content （

0.05）， down-regulated the mRNA expression of TGF-

， Smad2， and Smad3 in hippocampal tissues， and up-regulated the Smad7 mRNA expression （

0.05）. The comparison with the high-dose Bushen Shugan prescription group showed that the SOD and GSH contents in hippocampal tissues of mice in the medium- and low-dose Bushen Shugan prescription groups declined significantly， while the MDA contents rose significantly （

0.05）. Besides， the mRNA expression levels of TGF-

， Smad2 and Smad3 in the hippocampal tissues of mice in the medium- and low-dose Bushen Shugan prescription groups were significantly up-regulated， and those of Smad7 were significantly down-regulated （

0.05）.

Conclusion

Bushen Shugan prescription alleviates the depression symptoms in aged SAPM8 mice possibly by regulating the hippocampal oxidative stress and TGF-

/Smad signaling pathway.

关键词

Keywords

references

杨芙蓉，陈家旭，邹小娟，等 . 从肾阳亏虚浅议老年抑郁症与骨质疏松的相关性［J］. 中华中医药杂志， 2020 ， 35 （ 9 ）： 4398 - 4401 .

MOYLAN S ， BERK M ， DEAN O M ， et al . Oxidative & nitrosative stress in depression：why so much stress？［J］. Neurosci Biobehav Rev ， 2014 ， 45 ： 46 - 62 .

KOTAN V O ， SARANDOL E ， KIRHAN E ， et al . Effects of long-term antidepressant treatment on oxidative status in major depressive disorder：a 24-week follow-up study ［J］. Prog Neuropsychopharmacol Biol Psychiatry ， 2011 ， 35 （ 5 ）： 1284 - 1290 .

DREVETS W C ， PRICE J L ， FUREY M L . Brain structural and functional abnormalities in mood disorders：implications for neurocircuitry models of depression ［J］. Brain Struct Funct ， 2008 ， 213 （ 1/2 ）： 93 - 118 .

ARMSTRONG J S ， STEINAUER K K ， HORNUNG B ， et al . Role of glutathione depletion and reactive oxygen species generation in apoptotic signaling in a human B lymphoma cell line ［J］. Cell Death Differ ， 2002 ， 9 （ 3 ）： 252 - 263 .

SEKELSKY J J ， NEWFELD S J ， RAFTERY L A ， et al . Genetic characterization and cloning of mothers against dpp，a gene required for decapentaplegic function in Drosophila melanogaster ［J］. Genetics ， 1995 ， 139 （ 3 ）： 1347 - 1358 .

刘慧慧，马丹，赵丹玉，等 . 补肾填精方对抑郁样模型大鼠海马CaMKⅡ及CREB蛋白磷酸化水平的影响［J］. 时珍国医国药， 2020 ， 31 （ 3 ）： 575 - 579 .

孙珂焕，冯知涛，汤弋哲，等 . 柴胡疏肝散对抑郁模型大鼠行为学及Notch1信号通路的影响［J］. 中华行为医学与脑科学杂志， 2018 ， 27 （ 6 ）： 492 - 498

梁奕强，吴梅 . 柴胡舒肝散联合右佐匹克隆片治疗肝气郁结型焦虑症的临床观察［J］. 中国处方药， 2020 ， 18 （ 11 ）： 80 - 81 .

谭朝辉，陈尚茹，林健祥，等 . 柴胡疏肝汤加减治疗抑郁气郁证的临床疗效［J］. 内蒙古中医药， 2020 ， 39 （ 5 ）： 11 - 13 .

尹芳，王璐，金国琴 . 左归丸的临床应用与实验研究进展［J］. 江苏中医药， 2017 ， 49 （ 10 ）： 82 - 85 .

姚建平，李琳，牛巧能，等 . 左归丸对自然衰老大鼠海马乙酰胆碱含量及学习记忆功能的影响［J］. 中成药， 2018 ， 40 （ 1 ）： 181 - 183 .

杨振博 . 补肾疏肝方对老年抑郁症模型大鼠HPA轴功能调节作用的研究［D］. 郑州：河南中医药大学， 2018 .

黄继汉，黄晓晖，陈志扬，等 . 药理试验中动物间和动物与人体间的等效剂量换算［J］. 中国临床药理学与治疗学， 2004 ， 9 （ 9 ）： 1069 - 1072 .

TAGLIARI B ， DOS SANTOS T M ， CUNHA A A ， et al . Chronic variable stress induces oxidative stress and decreases butyrylcholinesterase activity in blood of rats ［J］. J Neural Transm （Vienna）， 2010 ， 117 （ 9 ）： 1067 - 1076 .

SAMOUILIDOU E C ， GRAPSA E J ， KAKAVAS I ， et al . Oxidative stress markers and C-reactive protein in end-stage renal failure patients on dialysis ［J］. Int Urol Nephrol ， 2003 ， 35 （ 3 ）： 393 - 397 .

MAES M ， GALECKI P ， CHANG Y S ， et al . A review on the oxidative and nitrosative stress （O&NS） pathways in major depression and their possible contribution to the （neuro）degenerative processes in that illness ［J］. Prog Neuropsychopharmacol Biol Psychiatry ， 2011 ， 35 （ 3 ）： 676 - 692 .

阙文兵，补肾活血汤联合中医情志疗法治疗老年肾虚肝郁型抑郁症的临床疗效分析［J］. 齐齐哈尔医学院学报， 2019 ， 40 （ 6 ）： 719 - 720 .

唐新辉，李春玲，尚淑梅，等 . 解郁胶囊对脑梗死后抑郁症患者神经功能的影响［J］. 中西医结合心脑血管病杂志， 2013 ， 11 （ 7 ）： 822 - 823 .

AKHURST R J ， HATA A . Targeting the TGFbeta signalling pathway in disease ［J］. Nat Rev Drug Discov ， 2012 ， 11 （ 10 ）： 790 - 811 .

李菲，陈慧彬，吴帅，等 . 转化生长因子- β 1 与硒蛋白P在慢性应激性抑郁发生中的作用及关系［J］. 陕西师范大学学报：自然科学版， 2014 ， 42 （ 4 ）： 57 - 64 .

窦建卫，王晓燕，王静怡，等 . 养心开郁片对抑郁模型动物行为和血清TGF- β 1 的影响［J］. 广西中医药， 2015 ， 38 （ 6 ）： 74 - 76 .

BRUNO V ， BATTAGLIA G ， CASABONA G ， et al . Neuroprotection by glial metabotropic glutamate receptors is mediated by transforming growth factor-beta ［J］. J Neurosci ， 1998 ， 18 （ 23 ）： 9594 - 9600 .

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Effect of Bushen Shugan Prescription on Oxidative Stress and TGF-β1/Smad Signaling Pathway in Hippocampus of Senile Depressed Mice

DOI：10.13422/j.cnki.syfjx.20212002

摘要

Abstract

关键词

Keywords

references

Effect of Bushen Shugan Prescription on Oxidative Stress and TGF-β₁/Smad Signaling Pathway in Hippocampus of Senile Depressed Mice