Efficacy of Jianpi Xiaoai Prescription in Inhibition of Metastasis of Colon Cancer HCT116 Cells： An Exploration Based on LncRNA HOTAIR/JAK2/STAT3 Signaling Pathway

Jiao JIAO; Qi TANG; Yi-lan JIANG; Dong-fang LI; Jia-qin LIU; Guang-sheng HU

doi:10.13422/j.cnki.syfjx.20212325

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Efficacy of Jianpi Xiaoai Prescription in Inhibition of Metastasis of Colon Cancer HCT116 Cells： An Exploration Based on LncRNA HOTAIR/JAK2/STAT3 Signaling Pathway

更新时间：2021-10-29

- Efficacy of Jianpi Xiaoai Prescription in Inhibition of Metastasis of Colon Cancer HCT116 Cells： An Exploration Based on LncRNA HOTAIR/JAK2/STAT3 Signaling Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 23, Pages: 66-71(2021)
- 作者机构：
  
  1.湖南中医药大学研究生院，长沙 410208
  2.湖南省肿瘤医院，长沙 410013
  3.湖南省中医药研究院附属医院，长沙 410006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20212325
  CLC： R22;R242;R2-031;R285.5
- Received：14 September 2021，
  
  Published Online：15 October 2021，
  
  Published：05 December 2021
- 稿件说明：
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焦蕉,唐麒,蒋益兰等.健脾消癌方通过lncRNA HOTAIR/JAK2/STAT3信号通路抑制结肠癌细胞株HCT116转移的机制[J].中国实验方剂学杂志,2021,27(23):66-71.

JIAO Jiao,TANG Qi,JIANG Yi-lan,et al.Efficacy of Jianpi Xiaoai Prescription in Inhibition of Metastasis of Colon Cancer HCT116 Cells： An Exploration Based on LncRNA HOTAIR/JAK2/STAT3 Signaling Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(23):66-71.
焦蕉,唐麒,蒋益兰等.健脾消癌方通过lncRNA HOTAIR/JAK2/STAT3信号通路抑制结肠癌细胞株HCT116转移的机制[J].中国实验方剂学杂志,2021,27(23):66-71. DOI： 10.13422/j.cnki.syfjx.20212325.

JIAO Jiao,TANG Qi,JIANG Yi-lan,et al.Efficacy of Jianpi Xiaoai Prescription in Inhibition of Metastasis of Colon Cancer HCT116 Cells： An Exploration Based on LncRNA HOTAIR/JAK2/STAT3 Signaling Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(23):66-71. DOI： 10.13422/j.cnki.syfjx.20212325.

摘要

目的

观察健脾消癌方对Hox转录本反义基因间RNA（lncRNA HOTAIR）/ 酪氨酸蛋白激酶2（JAK2）/信号传导及转录激活因子3（STAT3）信号通路的影响，探讨健脾消癌方抑制结肠癌转移的可能作用机制。

方法

分析不同细胞株lncRNA HOTAIR的表达情况，采用10%，15%，20%健脾消癌方含药血清作用于HCT116细胞，transwell实验检测健脾消癌方对HCT116细胞的侵袭能力影响，实时荧光定量聚合酶链式反应（Real-time PCR）检测lncRNA HOTAIR，JAK2，STAT3 mRNA的表达，蛋白免疫印迹法（Western blot）检测JAK2，磷酸化STAT3（p-STAT3），STAT3蛋白表达情况。

结果

结肠癌细胞株HCT116中lncRNA HOTAIR的表达水平最高，采用此细胞作为观察对象。与空白组比较，健脾消癌方各组的侵袭细胞数均降低（

0.05，

0.01）；健脾消癌方中剂量组JAK2 mRNA相对表达水平降低（

0.05）；健脾消癌方中、高剂量组的lncRNA HOTAIR，健脾消癌方高剂量组的JAK2 mRNA相对表达水平显著降低（

0.01）。与空白组比较，健脾消癌方中剂量组的p-STAT3蛋白相对表达水平均降低（

0.05）；健脾消癌方中、高剂量组的JAK2蛋白，健脾消癌方高剂量组的p-STAT3蛋白相对表达水平均明显降低（

0.01）。

结论

健脾消癌方可有效抑制结肠癌细胞转移，其机制可能与抑制lncRNA HOTAIR/JAK2/STAT3信号通路的表达相关。

Abstract

Objective

To observe the effect of Jianpi Xiaoai prescription on long non-coding RNA Hox transcript antisense intergenic RNA （lncRNA HOTAIR）/Janus kinase 2 （JAK2） /signal transducer and activator of transcription 3 （STAT3） signaling pathway and to explore the potential mechanism of Jianpi Xiaoai prescription in suppressing the metastasis of colon cancer.

Method

The expression of lncRNA HOTAIR in different cells was analyzed. Following the treatment of HCT116 cells with 10%，15%，and 20% Jianpi Xiaoai prescription -containing serum， the invasive ability of Jianpi Xiaoai prescription on HCT116 cells was assessed by transwell assay. The mRNA expression levels of lncRNA HOTAIR，JAK2，and STAT3 were measured by Real-time polymerase chain reaction （Real-time PCR）， and the protein expression levels of JAK2， phosphorylated STAT3 （p-STAT3） and STAT3 by Western blot.

Result

The highest expression of lncRNA HOTAIR was detected in HCT116 cells. Compared with the blank group， each Jianpi Xiaoai prescription group exhibited a decreased number of invasive cells （

0.05，

0.01）. The relative JAK2 mRNA expression in the middle-dose Jianpi Xiaoai prescription group was down-regulated （

0.05）， and the relative lncRNA HOTAIR mRNA expression in the middle- and high-dose Jianpi Xiaoai prescription groups and the relative JAK2 mRNA expression in the high-dose Jianpi Xiaoai prescription group were remarkably down-regulated （

0.01）. Compared with the blank group，the relative p-STAT3 protein expression was down-regulated in the middle-dose Jianpi Xiaoai prescription group （

0.05）， and the relative JAK2 protein expression in the middle- and high-dose Jianpi Xiaoai prescription groups and the relative p-STAT3 protein expression in the high-dose Jianpi Xiaoai prescription group were remarkably down-regulated （

0.01）.

Conclusion

Jianpi Xiaoai prescription effectively inhibits the metastasis of colon cancer cells， which may be related to the inhibition of lncRNA HOTAIR/JAK2/STAT3 signaling pathway.

关键词

Keywords

references

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