CHEN Cui-ping,XU Hong-xia,DONG Shuai,et al.Cryopreservation Conditions of Carthamus tinctorius Seeds[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(03):155-162.
CHEN Cui-ping,XU Hong-xia,DONG Shuai,et al.Cryopreservation Conditions of Carthamus tinctorius Seeds[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(03):155-162. DOI: 10.13422/j.cnki.syfjx.20220118.
Cryopreservation Conditions of Carthamus tinctorius Seeds
To study on the suitable cryopreservation conditions of
Carthamus tinctorius
seeds.
Method
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The germination rate,relative conductivity,soluble sugar,soluble protein, and related enzyme activities of
C. tinctorius
seeds, as well as the hydroxysafflor yellow A (HSYA) content in Carthami Flos after storage and breeding for four months were detected under different temperature conditions (long-term storage,medium-term storage,short-term storage,room temperature,and ultra-low temperature refrigerator),different water content (8.1%,6.6%,5.2%,and 3.9%),and different storage time (2,4,6,8, 10 months). SPSS 20.0 was used for statistical analysis.
Result
2
During the storage for 10 months,the changing trend of the germination rate of
C. tinctorius
seeds revealed that it was more suitable to store seeds with low water content at a lower temperature. The differences in germination rate of seeds caused by storage temperature,seeds water content, and storage time were statistically significant. After storage for 10 months,the germination rate was significantly correlated with other detection indexes.
Conclusion
2
The proper water content of
C. tinctorius
seeds in long-term and medium-term storage is 5.2% or 6.6%,and that in short-term and ultra-low temperature refrigerator is 3.9% or 5.2%. As revealed by the comparison results, the optimal storage conditions for
C. tinctorius
seeds were long-term storage and water content of 5.2%, which resulted in the highest germination rate and content of soluble sugar and soluble protein and the lowest relative conductivity after storage for 10 months. Additionally, the content of hydroxy safflor yellow A (HSYA) in Carthami Flos obtained after breeding and regeneration for four months was higher than that obtained after room temperature storage.
HONG T D , ELLIS R H . A protocol to determine seed storage behavior . IPGRI Technical Bulletin No .1[M]. Rome : International Plant Genetic RESOURCES Institute , 1996 .