Objective

2

To study the

in vitro

kinetics of Jiaojiang cataplasms and evaluate its pharmacodynamics， so as to provide a feasible basis for the development of this preparation.

Method

2

The improved Franz diffusion cell was used for the

in vitro

release in semipermeable membrane and transdermal absorption in

in vitro

mouse skins. The contents of hydroxy-

α

-sanshool， 6-gingerol， ginsenoside Rb

1

were determined by high performance liquid chromatography （HPLC）， to evaluate the

in vitro

release and transdermal absorption of Jiaojiang cataplasms. The mobile phase of 6-gingerol and hydroxy-

α

-sanshool was water-acetonitrile-methanol （2∶1∶1） with the detection wavelength of 280 nm. The mobile phase of ginsenoside Rb

1

was acetonitrile-0.1% phosphoric acid aqueous solution （31∶69） with the detection wavelength of 203 nm. A mouse intestinal paralysis model was established， and mice were randomly divided into five groups， namely sham operation group， model group， domperidone group （3.9 mg·kg

-1

） and high- and low-dose groups of Jiaojiang cataplasms （6.2， 3.1 g·kg

-1

， measured by crude drug dosage）， to observe the effect of this preparation on gastrointestinal propulsion function.

Result

2

Average release rates of hydroxy-

α

-sanshool， 6-gingerol and ginsenoside Rb

1

at 24 h were 16.41， 4.23， 4.15 μg∙cm

-2

∙h

-1

， the average transdermal rates of them at 24 h were 2.31， 0.64， 0.29 μg∙cm

-2

∙h

-1

， their skin retention values were 19.56， 3.59， 1.61 μg， respectively. According to the Ritger-Peppas equation， the release of hydroxy-

α

-sanshool， 6-gingerol， ginsenoside Rb

1

was non-Fick diffusion. The high-dose group of Jiaojiang cataplasms could improve intestinal function of model mice after small intestinal friction injury， and promote intestinal peristalsis and small intestinal propulsion rate （

P

<

0.05）.

Conclusion

2

Jiaojiang cataplasms has

in vitro

release and transdermal properties， the

in vitro

release conforms to Higuchi equation， and transdermal absorption behavior conforms to zero-order kinetic equation， which can improve the postoperative function of the small intestine and the propulsion function of small intestine. It preliminarily indicates that the preparation has certain clinical development value.