Effect of Xiangsha Liu Junzitang on Gastric Motility and CRF and UCN2 Expression in Rats with Functional Dyspepsia Due to Spleen Deficiency

Juan-juan LI; Feng-yun WANG; Lin LYU; Lin XU; Xu LIANG; Xu-dong TANG

doi:10.13422/j.cnki.syfjx.20220337

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Effect of Xiangsha Liu Junzitang on Gastric Motility and CRF and UCN2 Expression in Rats with Functional Dyspepsia Due to Spleen Deficiency

更新时间：2021-12-31

- Effect of Xiangsha Liu Junzitang on Gastric Motility and CRF and UCN2 Expression in Rats with Functional Dyspepsia Due to Spleen Deficiency
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 3, Pages: 1-7(2022)
- 作者机构：
  
  1.深圳市中医院，广东深圳 518033
  2.中国中医科学院西苑医院，北京 100091
  3.中国中医科学院，北京 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220337
  CLC： R2-0;R289;R33;R318.14
- Received：18 September 2021，
  
  Published Online：30 November 2021，
  
  Published：05 February 2022
- 稿件说明：
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李娟娟,王凤云,吕林等.香砂六君子汤对功能性消化不良脾虚证大鼠胃动力及CRF，UCN2表达的影响[J].中国实验方剂学杂志,2022,28(03):1-7.

LI Juan-juan,WANG Feng-yun,LYU Lin,et al.Effect of Xiangsha Liu Junzitang on Gastric Motility and CRF and UCN2 Expression in Rats with Functional Dyspepsia Due to Spleen Deficiency[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(03):1-7.
李娟娟,王凤云,吕林等.香砂六君子汤对功能性消化不良脾虚证大鼠胃动力及CRF，UCN2表达的影响[J].中国实验方剂学杂志,2022,28(03):1-7. DOI： 10.13422/j.cnki.syfjx.20220337.

LI Juan-juan,WANG Feng-yun,LYU Lin,et al.Effect of Xiangsha Liu Junzitang on Gastric Motility and CRF and UCN2 Expression in Rats with Functional Dyspepsia Due to Spleen Deficiency[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(03):1-7. DOI： 10.13422/j.cnki.syfjx.20220337.

摘要

目的

观察香砂六君子汤对功能性消化不良（FD）脾虚证大鼠胃排空率、促肾上腺皮质激素释放因子（CRF）和尿皮质素2（UCN2）表达的影响。

方法

将48只10日龄SD雄性乳鼠随机分为正常组（8只）和碘乙酰胺组（40只），分别给予2%蔗糖溶液和0.1%蔗糖碘乙酰胺溶液灌胃，连续6 d。至出生3周龄，剔除母鼠，碘乙酰胺组大鼠随机分为模型组、莫沙必利组、香砂六君子汤低、中、高剂量组，每组8只。至出生6周龄，除正常组外，其余5组大鼠叠加小平台站立方法造模，共14 d。造模结束后，正常组和模型组给予蒸馏水10 mL·kg

-1

，各治疗组分别给予莫沙必利1.6×10

-3

g·kg

-1

及香砂六君子汤水煎液2.8，5.6，11.2 g·kg

-1

灌胃，共14 d。测定抓力、胃排空率，采用苏木素-伊红（HE）染色法观察胃窦基本形态，采用蛋白免疫印迹法（Western blot）和实时荧光定量聚合酶链式反应（Real-time PCR）检测胃窦组织CRF，UCN2蛋白及mRNA表达量。

结果

各组大鼠胃窦形态基本正常，未见器质性病变；与正常组比较，模型组大鼠抓力、胃排空率显著降低（

0.01），胃窦组织CRF蛋白与mRNA表达显著升高（

0.01），UCN2蛋白与mRNA表达明显降低（

0.05，

0.01）；与模型组比较，香砂六君子中、高剂量组大鼠抓力、胃排空率明显升高（

0.05，

0.01），香砂六君子高剂量组CRF蛋白表达明显降低（

0.05），香砂六君子中、高剂量组CRF mRNA表达均有不同程度降低（

0.05，

0.01），香砂六君子高剂量组UCN2蛋白与mRNA表达显著升高（

0.01）。

结论

香砂六君子汤可发挥健脾、促进FD胃动力的作用，机制可能与降低胃组织CRF，升高UCN2的表达有关。

Abstract

Objective

To observe the effects of Xiangsha Liu Junzitang （XSLJZ） on gastric emptying rate and expression levels of corticotropin-releasing factor （CRF） and urocortin 2 （UCN2） in rats with functional dyspepsia （FD） due to spleen deficiency.

Method

Forty-eight 10-day-old male SD rats were randomly divided into the normal group （

=8） and iodoacetamide （IA） group （

=40）， and they separately received 2% sucrose solution and 0.1% sucrose solution containing IA for six successive days. Following the removal of mother rats， the three-week-old IA-treated rats were randomized into five groups， namely the model group， mosapride group， and low-， middle-， and high-dose XSLJZ groups， with eight rats in each group. At the age of six weeks， rats in all groups expert for the normal group were modeled by the modified multiple platform method for 14 d. Afterwards， the ones in normal group and model group were treated with 10 mL·kg

-1

distilled water， and those in the treatment groups with 1.6×10

-3

g·kg

-1

mosapride and 2.8， 5.6， and 11.2 g·kg

-1

XSLJZ by gavage， respectively， for 14 d. The grasping ability and gastric emptying rate were determined. The histological changes in gastric antrum were observed by hematoxylin-eosin （HE） staining. The protein and mRNA expression levels of CRF and UCN2 in gastric antrum were detected by Western blot and real-time fluorescent quantitative polymerase chain reaction （Real-time PCR）.

Result

No obvious change or organic lesion was observed in gastric antrum of rats in each group. Compared with the normal group， the model group exhibited lowered gastric emptying rate and grasping ability （

0.01）， up-regulated CRF protein and mRNA expression in gastric antrum （

0.01）， and down-regulated UCN2 protein and mRNA expression （

0.05，

0.01）. Compared with the model group， XSLJZ at the middle and high doses enhanced the grasping ability and gastric emptying rate （

0.05，

0.01） and down-regulated CRF mRNA expression to varying degrees （

0.05，

0.01）. XSLJZ at the high dose decreased CRF protein expression （

0.05） and up-regulated UCN2 protein and mRNA expression （

0.01）.

Conclusion

The mechanism of XSLJZ in invigorating spleen and promoting gastric motility of FD rats may be related to its reduction of CRF and elevation of UCN2 in gastric antrum.

关键词

Keywords

references

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