Effect of Naozhenning on Hippocampal Mitochondrial Function in Model Rats with Post-concussion Syndrome

Xiao-zhi JIANG; Le ZHAO; Qian WANG; Bei-xi TAN; Chao-qun WANG; Yong-hui WANG

doi:10.13422/j.cnki.syfjx.20220409

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Effect of Naozhenning on Hippocampal Mitochondrial Function in Model Rats with Post-concussion Syndrome

更新时间：2022-04-28

- Effect of Naozhenning on Hippocampal Mitochondrial Function in Model Rats with Post-concussion Syndrome
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 11, Pages: 86-92(2022)
- 作者机构：
  
  1.山西中医药大学，山西晋中 030619
  2.湖北中医药大学，武汉 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220409
  CLC： R2-0;R22;R285.5;R289;R33
- Received：19 October 2021，
  
  Published Online：22 December 2021，
  
  Published：05 June 2022
- 稿件说明：
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江晓智,赵乐,王钱等.脑震宁对脑震荡后综合征模型大鼠海马线粒体功能的影响[J].中国实验方剂学杂志,2022,28(11):86-92.

JIANG Xiao-zhi,ZHAO Le,WANG Qian,et al.Effect of Naozhenning on Hippocampal Mitochondrial Function in Model Rats with Post-concussion Syndrome[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(11):86-92.
江晓智,赵乐,王钱等.脑震宁对脑震荡后综合征模型大鼠海马线粒体功能的影响[J].中国实验方剂学杂志,2022,28(11):86-92. DOI： 10.13422/j.cnki.syfjx.20220409.

JIANG Xiao-zhi,ZHAO Le,WANG Qian,et al.Effect of Naozhenning on Hippocampal Mitochondrial Function in Model Rats with Post-concussion Syndrome[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(11):86-92. DOI： 10.13422/j.cnki.syfjx.20220409.

摘要

目的

基于线粒体功能，探究脑震宁对脑震荡后综合征模型大鼠学习记忆能力及海马CA1区神经元损伤的作用机制。

方法

SPF级Wistar大鼠，采用自由落体撞击法制备三重脑震荡后综合征模型（MCC），将造模成功的大鼠按随机数字表法分成5组，模型组、吡拉西坦组、脑震宁低、中、高剂量组，另设正常组，每组10只。吡拉西坦组及脑震宁低、中、高剂量组分别以0.43、5.4、10.8、21.6 g·kg

-1

的剂量灌胃给药，正常组和模型组大鼠分别灌服等体积蒸馏水，连续给药14 d进行相关指标检测。采用Morris水迷宫实验观察各组大鼠逃避潜伏期、原象限停留时间及穿越平台次数等学习记忆改变情况；苏木素-伊红（HE）染色、尼氏染色观察海马CA1区病理形态学改变；透射电子显微镜（TEM）观察线粒体超微结构；比色法检测线粒体呼吸链复合体Ⅰ活性；荧光探针法测定腺嘌呤核苷三磷酸（ATP）含量；酶联荧光免疫技术法检测线粒体膜电位（MMP）。

结果

与正常组比较，模型组大鼠逃避潜伏期时间明显延长，目标象限停留时间及穿越平台次数明显减少，海马CA1区神经元和尼氏小体数量减少，神经元形态及线粒体结构遭受破坏，线粒体膜电位、ATP和呼吸链复合体Ⅰ含量明显减少（

0.05，

0.01）；与模型组比较，脑震宁各剂量组大鼠逃避潜伏期明显缩短，目标象限停留时间及穿越平台次数明显增加，海马CA1区神经元及尼氏小体数量显著增加，神经元形态及线粒体结构损伤有所改善，线粒体膜电位、ATP和呼吸链复合体Ⅰ含量明显增加（

0.05，

0.01），脑震宁低剂量组大鼠穿越平台次数及线粒体呼吸链复合体Ⅰ差异无统计学意义。

结论

脑震宁可以改善MCC大鼠学习记忆能力，其机制可能与改善线粒体结构与功能，减轻海马神经元损伤有关。

Abstract

Objective

To explore the mechanism of Naozhenning on learning and memory ability and neuron damage in hippocampal CA1 region of post-concussion syndrome model rats based on mitochondrial function.

Method

Multiple cerebral concussion （MCC） was induced in SPF Wistar rats with the free-fall impact method. Then the model rats were randomly classified into model group （equivalent volume of distilled water）， piracetam （0.43 g·kg

-1

，

） group， and low-， medium-， and high-dose NZN （5.4， 10.8， 21.6 g·kg

-1

， respectively，

） groups， with 10 rats in each group， and another 10 normal rats were included in the normal control group （equivalent volume of distilled water）. The administration lasted 14 days and then relevant indexes were detected. Morris water maze test was used to observe the changes of learning and memory ability in each group， such as escape latency， residence time in primary quadrant， and times of crossing platform. The pathological changes of hippocampal CA1 region were observed based on hematoxylin-eosin （HE） staining and Nissl staining. The ultrastructure of mitochondria was observed under the transmission electron microscope （TME） and the activity of mitochondrial respiratory chain complex Ⅰ was detected by colorimetry. The content of adenosine triphosphate （ATP） was determined by fluorescence probe and mitochondrial membrane potential （MMP） by fluorescein enzyme-linked fluorescence immunoassay.

Result

Compared with the normal control group， the model group showed long escape latency， short residence time in target quadrant， few times of crossing the platform， significant decrease in counts of neurons and Nissl bodies in hippocampal CA1 region， damage of neuronal morphology and mitochondrial structure， and significant reduction of MMP and the content of mitochondrial ATP and respiratory chain complex I （

0.05，

0.01）. The NZN groups demonstrated short escape latency， long residence time in target quadrant， increased times of crossing the platform， small number of neurons and Nissl bodies in hippocampal CA1 region， alleviated damage of neuronal morphology and mitochondrial structure， and increase in MMP and the content of mitochondrial ATP and respiratory chain complex I （

0.05，

0.01）.

Conclusion

NZN can improve the learning and memory ability of MCC rats by improving mitochondrial structure and function and alleviating hippocampal neuron injury.

关键词

Keywords

references

DE FREITAS CARDOSO M G ， FALEIRO R M ， DE PAULA J J ， et al . Cognitive impairment following acute mild traumatic brain injury ［J］. Frontiers in neurology ， 2019 ， 10 ： 198 .

BECKER R E ， KAPOGIANNIS D ， GREIG N H . Does traumatic brain injury hold the key to the Alzheimer's disease puzzle？［J］. Alzheimers Dement ， 2018 ， 14 （ 4 ）： 431 - 443 .

JAFARI S ， ETMINAN M ， AMINZADEH F ， et al . Head injury and risk of Parkinson disease： A systematic review and Meta-analysis ［J］. Mov Disord ， 2013 ， 28 （ 9 ）： 1222 - 1229 .

THOMAS D G ， APPS J N ， HOFFMANN R G ， et al . Benefits of strict rest after acute concussion： A randomized controlled trial ［J］. Pediatrics ， 2015 ， 135 （ 2 ）： 213 - 223 .

CONIDI F X . Interventional treatment for post-traumatic headache ［J］. Curr Pain Headache Rep ， 2016 ， 20 （ 6 ）： 40 .

ASHBAUGH A ， MCGREW C . The role of nutritional supplements in sports concussion treatment ［J］. Curr Sports Med Rep ， 2016 ， 15 （ 1 ）： 16 - 19 .

CARLSON S W ， SAATMAN K E . Central infusion of insulin-like growth factor-1 increases hippocampal neurogenesis and improves neurobehavioral function after traumatic brain injury ［J］. J Neurotrauma ， 2018 ， 35 （ 13 ）： 1467 - 1480 .

SANTIAGO-CASTAÑEDA C ， SEGOVIA-OROPEZA M ， CONCHA L ， et al . Propylparaben reduces the long-term consequences in hippocampus induced by traumatic brain injury in rats： Its implications as therapeutic strategy to prevent neurodegenerative diseases ［J］. J Alzheimers Dis ， 2021 ， 82 （ s1 ）： s215 - s226 .

HUBBARD W B ， JOSEPH B ， SPRY M ， et al . Acute mitochondrial impairment underlies prolonged cellular dysfunction after repeated mild traumatic brain injuries ［J］. J Neurotrauma ， 2019 ， 36 （ 8 ）： 1252 - 1263 .

王学建，季炜鹏 . 脑震宁治疗脑外伤后头痛53例疗效观察［J］. 中华神经创伤外科电子杂志， 2017 ， 3 （ 5 ）： 292 - 293 .

梁善勇，王伟伟，张华志 . 脑震宁、谷维素、脑益嗪口服及耳针疗法治疗脑外伤综合征观察［J］. 中国医药导报， 2008 ， 5 （ 5 ）： 35 - 36 .

CAO J ， DUAN Y ， LIU Y ， et al . Metabolomics coupled with SystemsDock reveal the protective effect and the potential active components of Naozhenning granule against traumatic brain injury ［J］. J Ethnopharmacol ， 2020 ， 246 ： 112247 .

MYCHASIUK R ， FARRAN A ， ANGOA-PEREZ M ， et al . A novel model of mild traumatic brain injury for juvenile rats ［J］. J Vis Exp ， 2014 ，（ 94 ）： e51820 .

MYCHASIUK R ， FARRAN A ， ESSER M J . Assessment of an experimental rodent model of pediatric mild traumatic brain injury ［J］. J Neurotrauma ， 2014 ， 31 （ 8 ）： 749 - 757 .

刘芳，黑常春，孔斌，等 . 卵巢移植对去势大鼠海马神经元和雌激素膜受体GPR30表达的影响［J］. 基础医学与临床， 2015 ， 35 （ 7 ）： 889 - 893 .

LU L H ， BOWLES A O ， KENNEDY J E ， et al . Single-item versus multiple-item headache ratings in service members seeking treatment for brain injury ［J］. Mil Med ， 2020 ， 185 （ 1/2 ）： e43 - e46 .

LU L H ， REID M W ， COOPER D B ， et al . Sleep problems contribute to post-concussive symptoms in service members with a history of mild traumatic brain injury without posttraumatic stress disorder or major depressive disorder ［J］. NeuroRehabilitation ， 2019 ， 44 （ 4 ）： 511 - 521 .

HO R A ， HALL G B ， NOSEWORTHY M D ， et al . Post-concussive depression： Evaluating depressive symptoms following concussion in adolescents and its effects on executive function ［J］. Brain Inj ， 2020 ， 34 （ 4 ）： 520 - 527 .

OLDENBURG C ， LUNDIN A ， EDMAN G ， et al . Cognitive reserve and persistent post-concussion symptoms-A prospective mild traumatic brain injury （mTBI） cohort study ［J］. Brain Inj ， 2016 ， 30 （ 2 ）： 146 - 155 .

张华志，刘豪，盛永富 . 脑震宁、脑益嗪、谷维素结合治疗脑外伤后综合征568例［J］. 中华创伤杂志， 1998 ， 14 （ 1 ）： 55 .

范建新，刘艳之，刘海霞，等 . 脑震宁颗粒治疗脑外伤综合征的Meta分析［J］. 山西中医药大学学报， 2020 ， 21 （ 3 ）： 157 - 160 .

CHENG A ， HOU Y ， MATTSON M P . Mitochondria and neuroplasticity ［J］. ASN Neuro ， 2010 ， 2 （ 5 ）： e00045 .

王春玲，罗宁，蒋媛静，等 . 基于CaMkk β /AMPK通路介导线粒体自噬探讨敛肝熄风止颤方的神经保护机制［J］. 世界中医药， 2021 ， 16 （ 5 ）： 765 - 768，774 .

魏桂林，何青青，金强，等 . 线粒体损伤介导的药源性脏器损伤评价模型研究进展［J］. 世界中医药， 2020 ， 15 （ 23 ）： 3523 - 3535 .

BARKHOUDARIAN G ， HOVDA D A ， GIZA C C . The molecular pathophysiology of concussive brain injury ［J］. Clin Sports Med ， 2011 ， 30 （ 1 ）： 33 - 48 .

YONUTAS H M ， VEKARIA H J ， SULLIVAN P G . Mitochondrial specific therapeutic targets following brain injury ［J］. Brain Res ， 2016 ， 1640 （ Pt A ）： 77 - 93 .

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