Comparative Analysis of <italic style="font-style: italic">Lonicera japonica </italic>Flower<italic style="font-style: italic"> </italic>from Different Harvesting Periods Based on UV Fingerprint and Chemometrics

Ming-xia XIE; Jie-hua LI; Bo-hou XIA; Xi ZHANG; Qing-hua PENG; Xiao-ye WANG

doi:10.13422/j.cnki.syfjx.20220511

您当前的位置：

首页 >

文章列表页 >

Comparative Analysis of Lonicera japonica Flower from Different Harvesting Periods Based on UV Fingerprint and Chemometrics

更新时间：2022-03-07

- Comparative Analysis of Lonicera japonica Flower from Different Harvesting Periods Based on UV Fingerprint and Chemometrics
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 7, Pages: 134-141(2022)
- 作者机构：
  
  1.湖南中医药大学临床医学院，长沙 410208
  2.湖南省脑科医院（湖南省第二人民医院），长沙 410007
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220511
  CLC： R284.2;R289;R22;R2-031;R33
- Received：05 November 2021，
  
  Published Online：09 February 2022，
  
  Published：05 April 2022
- 稿件说明：
移动端阅览
谢明霞,李洁花,夏伯候等.基于UV-Vis指纹图谱结合化学计量学探讨不同采收期金银花的质量差异[J].中国实验方剂学杂志,2022,28(07):134-141.

XIE Ming-xia,LI Jie-hua,XIA Bo-hou,et al.Comparative Analysis of Lonicera japonica Flowerfrom Different Harvesting Periods Based on UV Fingerprint and Chemometrics[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(07):134-141.
谢明霞,李洁花,夏伯候等.基于UV-Vis指纹图谱结合化学计量学探讨不同采收期金银花的质量差异[J].中国实验方剂学杂志,2022,28(07):134-141. DOI： 10.13422/j.cnki.syfjx.20220511.

XIE Ming-xia,LI Jie-hua,XIA Bo-hou,et al.Comparative Analysis of Lonicera japonica Flowerfrom Different Harvesting Periods Based on UV Fingerprint and Chemometrics[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(07):134-141. DOI： 10.13422/j.cnki.syfjx.20220511.

摘要

目的

采用紫外-可见（UV-Vis）指纹图谱结合化学计量学方法，探讨不同采收期金银花药材的质量差异性。

方法

采集幼蕾期、青蕾期、白蕾期、银花期和金花期5个不同时期，共25个金银花样品，通过单因素优选确定最佳提取方法并在此条件下建立不同采收期金银花的UV-Vis指纹图谱。结果发现不同采收期的金银花样品的紫外指纹图谱在209、216、226、250、280、303、318、350 nm波长附近的吸光度均有显著差异。进一步将数据预处理、归一化等处理后，通过SIMCA-P

进行主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）、正交偏最小二乘判别分析（OPLS-DA）等多元统计分析方法，建立金银花5个不同采收期的质量差异性模型。

结果

PCA和PLS-DA结果均显示5个不同采收期的金银花样品各自聚在相近位置，且具有显著的采收时间依赖性趋势，说明金银花不同采收期间所含化学成分的含量差异较大，与采收期相关性较高。OPLS-DA两两比较分析表明引起幼蕾期到青蕾期变化的成分可能为三萜类或挥发油类，含量均减少；青蕾期到白蕾期变化的成分可能为三萜（或环烯醚萜）类、挥发油、酚酸类或黄酮类，含量降低，该结果与绿原酸HPLC测定结果一致；白蕾期至银花期变化的成分可能为含量增加的环烯醚萜类和含量减少的三萜（或挥发油）类，银花期至金花期变化的成分主要有含量增加的三萜（或挥发油）类。

结论

该研究方法具有简单，可操控性强等特点，可为中药质量控制方法的研究提供参考。

Abstract

Objective

To investigate the quality variation of

Lonicera japonica

flower from different harvesting periods by ultraviolet visible（UV-Vis） fingerprint combined with chemometrics.

Method

Twenty-five

L. japonica

flower samples from five harvesting periods， including young bud stage，green bud stage，white bud stage，silver and golden flower stages， were collected， with five samples for each stage. UV-Vis fingerprints of

L. japonica

flower from different harvesting periods were established in the context of the optimum extraction method based on the single factor experiment. The results showed that the absorption values at 209，216，226，250，280，303，318， and 350 nm were significantly different. Moreover，after data pretreatment and normalization，multivariate statistical analyses， such as principal component analysis （PCA），partial least squares discriminant analysis （PLS-DA），and orthogonal PLS-DA （OPLS-DA）were performed by SIMCA-P

to establish the quality variation model of

L. japonicas

flower from harvesting periods.

Result

As revealed by PCA and PLS-DA，

L. japonicas

flower samples from five harvesting periods were clustered separately and closely in a harvesting time-dependent manner， suggesting that the content of components contained in samples from different harvesting periods was highly distinct and correlated with harvesting periods. The pairwise comparison of OPLS-DA indicated that triterpenoids or volatile oils were the main components causing the changes from the young bud stage to the green bud stage，and the content of them decreased. The main components from the green bud stage to the white bud stage were triterpenoids （or iridoids），volatile oils，phenolic acids， or flavonoids，and the content of them decreased， which was consistent with the HPLC result of chlorogenic acid. From the white bud stage to the silver flower stage， the main components were iridoids （increasing in content） and triterpenoids （or volatile oils）（decreasing in content）. The main altered components from the silver flower stage to the golden flower stage were triterpenoids （or volatile oils） whose content increased.

Conclusion

This method is simple and feasible， which can provide references for the quality control of Chinese medicine.

关键词

Keywords

references

BIMBIRAITE-SURVILIENE K ， STANKEVICIUS M ， ŠUSTAUSKAITE S ， et al . Evaluation of chemical composition，radical scavenging and antitumor activities of Satureja hortensis L.Herb extracts ［J］. Antioxidants ， 2021 ， 10 （ 1 ）： 53 - 68 .

MOHAMMED H A ， AL-OMAR M S ， MOHAMMED S A A ， et al . Drying induced impact on composition and oil quality of rosemary herb， Rosmarinus officinalis Linn ［J］. Molecules ， 2020 ， 25 （ 12 ）： 2830 - 2842 .

国家药典委员会 . 中华人民共和国药典：一部［M］. 北京：中国医药科技出版社， 2020 ： 230 .

QIU S ， BAI M ， ZHAO P ， et al . Phytochemical and network-based chemotaxonomic study of Lonicera japonica Thunb ［J］. Biochem Syst Ecol ， 2021 ， 94 ： 104210 - 104215 .

KONG D X ， LI Y Q ， BAI M ， et al . Correlation between the dynamic accumulation of the main effective components and their associated regulatory enzyme activities at different growth stages in Lonicera japonica Thunb ［J］. Ind Crops Prod ， 2017 ， 96 ： 16 - 22 .

FU M ， QU Q ， DAI H . Variation in antioxidant properties and metabolites during flower maturation of Flos Lonicerae Japonicae flowers ［J］. Eur Food Res and Technol ， 2015 ， 240 （ 4 ）： 735 - 741 .

WORLD HEALTH ORGANIZATION . Guidelines for the assessment of herbal medicines ［M］. Geneva ： World Health Organization ， 1991 .

JIANG L ， DING H ， XU M ， et al . UV-Vis-NIR full-range responsive carbon dots with large multiphoton absorption cross sections and deep-red fluorescence at nucleoli and in vivo ［J］. Small ， 2020 ， 16 （ 19 ）： 2000680 - 2000689 .

SELAMNENI V ， GANESHAN S K ， SAHATIYA P . All MoS 2 based 2D/0D localized unipolar heterojunctions as flexible broadband （UV-vis-NIR） photodetectors ［J］. J Mater Chem C Mater ， 2020 ， 8 （ 33 ）： 11593 - 11602 .

RISWANTO F D O ， ROHMAN A ， PRAMONO S ， et al . The employment of UV-Vis spectroscopy and chemometrics techniques for analyzing the combination of genistein and curcumin ［J］. J Appl Pharm Sci ， 2021 ， 11 （ 3 ）： 154 - 161 .

ISMAIL A ， TORIMAN M E ， JUAHIR H ， et al . Spatial assessment and source identification of heavy metals pollution in surface water using several chemometric techniques ［J］. Mar Pollut Bull ， 2016 ， 106 （ 1/2 ）： 292 - 300 .

LóPEZ-DíEZ E C ， GOODACRE R . Characterization of microorganisms using UV resonance Raman spectroscopy and chemometrics ［J］. Anal Chem ， 2004 ， 76 （ 3 ）： 585 - 591 .

TOMAZZOLI M M ， PAI NETO R D ， MORESCO R ， et al . Discrimination of Brazilian propolis according to the seasoning using chemometrics and machine learning based on UV-Vis scanning data ［J］. J Integr Bioinform ， 2015 ， 12 （ 4 ）： 279 .

GAD H A ， EL-AHMADY S H ， ABOU-SHOER M I ， et al . A modern approach to the authentication and quality assessment of thyme using UV spectroscopy and chemometric analysis ［J］. Phytochem Anal ， 2013 ， 24 （ 6 ）： 520 - 526 .

BElAY A ， GHOLAP A V . Characterization and determination of chlorogenic acids （CGA） in coffee beans by UV-Vis spectroscopy ［J］. Afr J Pure Appl Chem ， 2009 ， 3 （ 11 ）： 34 - 240 .

SZOSTEK B ， ORSKA-GAWRYS J ， SUROWIEC I ， et al . Investigation of natural dyes occurring in historical Coptic textiles by high-performance liquid chromatography with UV-Vis and mass spectrometric detection ［J］. J Chromatogr A ， 2003 ， 1012 （ 2 ）： 179 - 192 .

LI L ， ZHANG J L ， SHENG Y X ， et al . Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method ［J］. J Pharm Biomed Anal ， 2005 ， 38 （ 1 ）： 45 - 51 .

PRADHAN A B ， HAQUE L ， BHUIYA S ， et al . Induction of self-structure in polyriboadenylic acid by the benzophenanthridine plant alkaloid chelerythrine：a spectroscopic approach ［J］. RSC Advances ， 2014 ， 4 （ 95 ）： 52815 - 52824 .

MEES C ， SOUARD F ， DELPORTE C ， et al . Identification of coffee leaves using FT-NIR spectroscopy and SIMCA ［J］. Talanta ， 2018 ， 177 ： 4 - 11 .

ABDI H ， WILLIAMS L J . Principal component analysis ［J］. Wiley Interdiscip Rev Comput Stat ， 2010 ， 2 （ 4 ）： 433 - 459 .

BALLABIO D ， CONSONNI V . Classification tools in chemistry.Part 1：linear models.PLS-DA ［J］. Anal Methods ， 2013 ， 5 （ 16 ）： 3790 - 3798 .

BYLESIO M ， RANTALAINEN M ， CLOAREC O ， et al . OPLS discriminant analysis：Combining the strengths of PLS‐DA and SIMCA classification ［J］. J Chemom Syst ， 2006 ， 20 （ 8/10 ）： 341 - 351 .

WIKLUND S ， JOHANSSON E ， SJÖSTRÖM L ， et al . Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models ［J］. Anal Chem ， 2008 ， 80 （ 1 ）： 115 - 122 .

FU M ， QU Q ， DAI H . Variation in antioxidant properties and metabolites during flower maturation of Flos Lonicerae Japonicae flowers ［J］. Eur Food Res Technol ， 2015 ， 240 （ 4 ）： 735 - 741 .

WANG L ， LI M ， YAN Y ， et al . Influence of flowering stage of Lonicera japonica Thunb.on variation in volatiles and chlorogenic acid ［J］. J Sci Food Agric ， 2009 ， 89 （ 6 ）： 953 - 957 .

JIANG M ， HAN Y Q ， ZHOU M G ， et al . The screening research of anti-inflammatory bioactive markers from different flowering phases of Flos Lonicerae Japonicae ［J］. PLoS One ， 2014 ， 9 （ 5 ）： e96214 .

GEANA E I ， CIUCURE C T ， APETREI C ， et al . Application of spectroscopic UV-Vis and FT-IR screening techniques coupled with multivariate statistical analysis for red wine authentication：Varietal and vintage year discrimination ［J］. Molecules ， 2019 ， 24 （ 22 ）： 4166 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis of Volatile Component Changes During Processing of Gardeniae Fructus Carbonisata by HS-GC-IMS Combined with Chemometrics

Identification of Alumen and Ammonium alum Based on XRD， FTIR， TG-DTA Combined with Chemometrics

Quality Evaluation of Chuanxiong Rhizoma Dispensing Granules Based on HPLC Specific Chromatogram and Two Reference Substances for Determination of Multiple Components

Investigation of Component Difference of Astragali Radix Before and After Rice Stir-frying by UPLC-Q-TOF-MS/MS Combined with Chemometrics

Quality Evaluation of Aurantii Fructus from Sichuan by Specific Chromatogram Combined with Chemometrics and Grey Correlation Analysis

Related Author

YAN Lin

SONG Yanan

JIA Zhe

LIU Yanping

ZHANG Cun

WANG Yun

WANG Bin

ZHANG Huangsheng

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

State Key Laboratory of Southwestern Chinese Medicine Resources，School of Pharmacy， Chengdu University of Traditional Chinese Medicine

Shandong Institute for Food and Drug Control

Shandong Province Technical Innovation Center of Traditional Chinese Medicine Treatment of Respiratory Diseases，Shandong Hongjitang Pharmaceutical Group Co. Ltd.

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Comparative Analysis of Lonicera japonica Flower from Different Harvesting Periods Based on UV Fingerprint and Chemometrics

DOI：10.13422/j.cnki.syfjx.20220511

摘要

Abstract

关键词

Keywords

references