Content Difference of Effective Components of Cynomorii Herba Between Different Producing Areas and Its Response to Environmental Factors

Rui ZHANG; Zhi-rong GU; Yan GUO; Mei QI; Xin LYU; Xiao-wen MAO; Bin GE

doi:10.13422/j.cnki.syfjx.20220512

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Content Difference of Effective Components of Cynomorii Herba Between Different Producing Areas and Its Response to Environmental Factors

更新时间：2022-03-07

- Content Difference of Effective Components of Cynomorii Herba Between Different Producing Areas and Its Response to Environmental Factors
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 7, Pages: 142-150(2022)
- 作者机构：
  
  1.甘肃中医药大学药学院，兰州 730000
  2.甘肃省人民医院，兰州 730000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220512
  CLC： R284.2;R289;R22;R2-031;R33
- Received：19 November 2021，
  
  Published Online：09 February 2022，
  
  Published：05 April 2022
- 稿件说明：
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张锐,顾志荣,郭燕等.锁阳有效成分含量的产地差异及对环境因子的响应规律[J].中国实验方剂学杂志,2022,28(07):142-150.

ZHANG Rui,GU Zhi-rong,GUO Yan,et al.Content Difference of Effective Components of Cynomorii Herba Between Different Producing Areas and Its Response to Environmental Factors[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(07):142-150.
张锐,顾志荣,郭燕等.锁阳有效成分含量的产地差异及对环境因子的响应规律[J].中国实验方剂学杂志,2022,28(07):142-150. DOI： 10.13422/j.cnki.syfjx.20220512.

ZHANG Rui,GU Zhi-rong,GUO Yan,et al.Content Difference of Effective Components of Cynomorii Herba Between Different Producing Areas and Its Response to Environmental Factors[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(07):142-150. DOI： 10.13422/j.cnki.syfjx.20220512.

摘要

目的

探讨锁阳中没食子酸、原儿茶酸、儿茶素、总黄酮、总多糖等有效成分的产地差异及有效成分与环境因子的相关性。

方法

采集甘肃、内蒙古、新疆、青海、宁夏5个省（自治区）的12个市（地区/州/旗）主产区的95批锁阳样本，记录海拔、经纬度、气候类型、年平均无霜期、年降雨量、全年日照时数、年平均气温、年平均蒸发量等地理-气候因子，采用高效液相色谱法（HPLC）测定锁阳中没食子酸、原儿茶酸、儿茶素等3种主要成分含量，优化并建立化学成分含量与各环境因子的相关性数学模型。

结果

皮尔逊（Pearson）相关性分析表明，锁阳中原儿茶酸含量随年平均无霜期和年平均气温升高而增加，儿茶素含量随年平均无霜期、全年日照时数和年平均蒸发量升高而增加，总多糖含量随海拔升高而降低。冗余分析（RDA）结果表明，年平均无霜期、年平均蒸发量、全年日照时数及海拔对锁阳有效成分含量的影响较大。曲线拟合表明，锁阳的最佳生长海拔800~2 000 m，最佳年平均无霜期80~110 d，最佳年降雨量110~300 mm，最佳全年日照时数2 400~3 000 h，最佳年平均气温2.2~8.8 ℃，最佳年平均蒸发量1 700~2 500 mm。

结论

锁阳有效成分含量具有显著的产地差异，对环境因子具有明确的响应规律，其适宜生长及栽培引种的地区为海拔高、年平均无霜期短、年降雨量少、平均蒸发量大、全年日照时数长、年平均气温低的区域。

Abstract

Objective

To explore the content difference of gallic acid，protocatechuic acid，catechin，total flavonoids， and total polysaccharides in Cynomorii Herba between different producing areas and the correlation between effective components and environmental factors.

Method

Ninety-five batches of Cynomorii Herba samples were collected from 12 major producing areas in five provinces （autonomous regions）， including Gansu，Inner Mongolia，Xinjiang，Qinghai， and Ningxia，and the geographical-climatic factors such as altitude，longitude and latitude，climate type，annual average frost-free period，annual rainfall，annual sunshine hours，annual average temperature， and annual average evaporation were recorded. The content of gallic acid，protocatechuic acid， and catechin in Cynomorii Herba was determined by high-performance liquid chromatography （HPLC），and the mathematical model of the correlation between the content of chemical components and environmental factors was optimized and established.

Result

Pearson correlation analysis showed that protocatechuic acid content in Cynomorii Herba increased with the increase in annual average frost-free period and annual average temperature， and catechin content increased with the increase in the annual average frost-free period，annual sunshine hours， and annual average evaporation，while total polysaccharides content decreased with the increase in altitude. Redundancy analysis （RDA） showed that the annual average frost-free period， annual average evaporation，annual sunshine hours， and altitude had great influences on the content of effective components in Cynomorii Herba. Curve fitting showed that the optimal conditions for the growth of Cynomorii Herba were as follows： altitude of 800-2 000 m，annual average frost-free period of 80-110 d， annual rainfall of 110-300 mm，annual sunshine hours of 2 400-3 000 h， annual average temperature of 2.2-8.8 ℃，and annual average evaporation of 1 700-2 500 mm.

Conclusion

The content of effective components in Cynomorii Herba is diverse in terms of producing areas and shows a clear response rule to environmental factors. The areas suitable for growing and artificial cultivation introduction are those with high altitude，short annual average frost-free period，low annual rainfall，large average evaporation，long sunshine hours， and low annual average temperature.

关键词

Keywords

references

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