Discussion on Treatment of Diarrhea of Intestinal Dampness-heat Syndrome and of Cold-dampness Disturbing Spleen Syndrome with Different Methods： Based on Intestinal Microbiota Function

Yuli LI; Yawei LIU; Zhoujin TAN

doi:10.13422/j.cnki.syfjx.20220918

您当前的位置：

首页 >

文章列表页 >

Discussion on Treatment of Diarrhea of Intestinal Dampness-heat Syndrome and of Cold-dampness Disturbing Spleen Syndrome with Different Methods： Based on Intestinal Microbiota Function

更新时间：2022-07-14

- Discussion on Treatment of Diarrhea of Intestinal Dampness-heat Syndrome and of Cold-dampness Disturbing Spleen Syndrome with Different Methods： Based on Intestinal Microbiota Function
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 16, Pages: 140-149(2022)
- 作者机构：
  
  湖南中医药大学，长沙 410208
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220918
  CLC： R285;R289;R22;R2-031;R33
- Received：06 April 2022，
  
  Published Online：15 June 2022，
  
  Published：20 August 2022
- 稿件说明：
移动端阅览
李玉丽,刘娅薇,谭周进.基于肠道菌群功能探究泄泻肠道湿热证和泄泻寒湿困脾证的“同病异治”[J].中国实验方剂学杂志,2022,28(16):140-149.

LI Yuli,LIU Yawei,TAN Zhoujin.Discussion on Treatment of Diarrhea of Intestinal Dampness-heat Syndrome and of Cold-dampness Disturbing Spleen Syndrome with Different Methods： Based on Intestinal Microbiota Function[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):140-149.
李玉丽,刘娅薇,谭周进.基于肠道菌群功能探究泄泻肠道湿热证和泄泻寒湿困脾证的“同病异治”[J].中国实验方剂学杂志,2022,28(16):140-149. DOI： 10.13422/j.cnki.syfjx.20220918.

LI Yuli,LIU Yawei,TAN Zhoujin.Discussion on Treatment of Diarrhea of Intestinal Dampness-heat Syndrome and of Cold-dampness Disturbing Spleen Syndrome with Different Methods： Based on Intestinal Microbiota Function[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):140-149. DOI： 10.13422/j.cnki.syfjx.20220918.

摘要

目的

研究泄泻肠道湿热证和寒湿困脾证在不同肠道生态位的菌群失调变化，为探索泄泻“同病异治”的治疗特色提供肠道菌群视角的客观依据。

方法

从美国国家生物技术信息中心（NCBI）数据库获取泄泻肠道湿热证和寒湿困脾证模型小鼠，以及方证相应方剂干预后模型小鼠的16S rRNA基因高通量测序数据，比较各组小鼠的肠道优势菌组成、菌群功能差异；同时运用Spearman分别计算各组菌群间的相关性，构建肠道菌群共现网络以研究菌群相互作用。

结果

泄泻肠道湿热证肠内容物菌群失调以

Muribaculum

、

Aerococcus

富集为特征，肠黏膜菌群失调则以革兰氏阴性菌

Neisseria

、

Capnocytophaga、Prevotella

富集为特征（

0.05），而葛根芩连汤治疗后不同肠道生态位菌群以

Lactobacillus

富集为特征，肠黏膜还伴有

Streptococcus

丰度增加。泄泻寒湿困脾证肠内容物菌群失调以

Lactobacillus

（

0.01）、

Clostridium sensu stricto 1

富集为特征，肠黏膜则以

Candidatus Arthromitus

、

Enterobacter

丰度增加为主（

0.05），而藿香正气散治疗后肠内容物以

Lactobacillus

富集为特征，而肠黏膜菌群特征体现在

Candidatus Arthromitus

、

Pseudomonas

、

Bacillus

富集。整体上，肠黏膜优势菌的差异贡献率高于肠内容物，且肠黏膜优势菌与其他菌的相互作用要多于肠内容物优势菌。

结论

不同中医证型泄泻的肠道菌群失调存在差异，方证相应调节菌群失调的疗效同样存在差别，且“同一病证”在不同肠道生态位的菌群失调也各具特征，提示肠道菌群可能是探索泄泻中医“同病异治”治疗特色的生物学基础之一。

Abstract

Objective

To study the changes of microbiota in different intestinal niches in the instance of diarrhea with intestinal dampness-heat syndrome and cold-dampness disturbing spleen syndrome， so as to provide objective evidence for treating diarrhea with different methods from the perspective of intestinal flora.

Method

The 16S rRNA gene high-throughput sequencing data of model mice with diarrhea of the two syndromes and the model mice after prescription intervention were retrieved from National Center for Biotechnology Information （NCBI）， and the intestinal dominant bacteria and microbial functions were compared among groups. Spearman's correlation coefficient among the microorganisms in each group was calculated and the co-occurrence networks of intestinal microbiota were constructed to study the interaction of the microbiota.

Result

The microbiota imbalance in intestinal contents of mice with diarrhea of intestinal dampness-heat syndrome was characterized by the enrichment of

Muribaculum

and

Aerococcus

， while the imbalance in intestinal mucosa was manifested by the enrichment of Gram-negative

Neisseria

，

Capnocytophaga

， and

Prevotella

（

0.05）. However， after the treatment with Gegen Qinliantang， the microbiota in two distinct ecosystems was characterized by the enrichment of

Lactobacillus

and the abundance of

Streptococcus

in intestinal mucosa was increased. The microbiota imbalance in intestinal contents of diarrhea with cold-dampness disturbing spleen syndrome was characterized by the enrichment of

Lactobacillus

（

0.01） and

Clostridium sensu stricto 1

， while the intestinal mucosa was dominated by the increase of

Candidatus arthromitus

and

Enterobacter

. However， after the treatment with Huoxiang Zhengqi powder， the intestinal contents were characterized by

Lactobacillus

enrichment， while the intestinal mucosal flora was featured by the enrichment of

C. arthromitus

，

Pseudomonas

， and

Bacillus

. Overall， the contribution of dominant bacteria in intestinal mucosa to the difference was higher than that in intestinal contents， and more dominant bacteria in the intestinal mucosa interacted with other bacteria.

Conclusion

Microbiota imbalance is different for diarrhea of different syndromes， and the therapeutic effects of corresponding prescriptions are also different. In addition， the microbiota imbalance has different characteristics between intestinal niches for mice with diarrhea of the same syndrome. Therefore， intestinal flora may be one of the biological bases for exploring the characteristics of "treating the diarrhea with different methods" in Chinese medicine.

关键词

Keywords

references

MIAZGA A ， OSIńSKI M ， CICHY W ， et al . Current views on the etiopathogenesis， clinical manifestation， diagnostics， treatment and correlation with other nosological entities of SIBO ［J］. Adv Med Sci ， 2015 ， 60 （ 1 ）： 118 - 124 .

周丽，徐派的，张红星 . 肠道菌群与常见功能性胃肠病相关性的研究进展［J］. 华中科技大学学报：医学版， 2020 ， 49 （ 6 ）： 756 - 760 .

张声生，王垂杰，李玉锋，等 . 泄泻中医诊疗专家共识意见（2017）［J］. 中医杂志， 2017 ， 58 （ 14 ）： 1256 - 1260 .

冉颖卓，邵鑫，刘晶 . 2型糖尿病湿热证胰岛素抵抗与肠道菌群相关性的研究进展［J］. 中国中西医结合杂志， 2022 ， 42 （ 4 ）： 508 - 512 .

WU M ， LI P ， LI J ， et al . The differences between luminal microbiota and mucosal microbiota in mice ［J］. J Microbiol Biotechnol ， 2020 ， 30 （ 2 ）： 287 - 295 .

DONG L N ， WANG J P ， LIU P ， et al . Faecal and mucosal microbiota in patients with functional gastrointestinal disorders：Correlation with toll-like receptor 2/toll-like receptor 4 expression ［J］. World J Gastroenterol ， 2017 ， 23 （ 36 ）： 6665 - 6673 .

DAI T ， ZHANG Y ， TANG Y ， et al . Identifying the key taxonomic categories that characterize microbial community diversity using full-scale classification：A case study of microbial communities in the sediments of Hangzhou Bay ［J］. FEMS Microbiol Ecol ， 2016 ， 92 （ 10 ）： 150 .

KR C . Nonparametric multivariate analyses of changes in community structure ［J］. Aust J Ecol ， 1993 ， 18 ： 117 - 143 .

HAMMER Ø ， HARPER D ， RYAN P D . PAST：Paleontological statistics software package for education and data analysis ［J］. Palaeontologia Electronica ， 2001 ， 4 （ 1 ）： 9 .

BENJAMINI Y ， KRIEGER A M ， YEKUTIELI D . Adaptive linear step-up procedures that control the false discovery rate ［J］. Biometrika ， 2006 ， 93 （ 3 ）： 491 - 507 .

LANGFELDER P ， HORVATH S . Fast R functions for robust correlations and hierarchical clustering ［J］. J Stat Softw ， 2012 ， 46 （ 11 ）： 11 .

DOUGLAS G M ， MAFFEI V J ， ZANEVELD J R ， et al . PICRUSt2 for prediction of metagenome functions ［J］. Nat Biotechnol ， 2020 ， 38 （ 6 ）： 685 - 688 .

CASPI R ， BILLINGTON R ， KESELER I M ， et al . The MetaCyc database of metabolic pathways and enzymes-a 2019 update ［J］. Nucleic Acids Res ， 2020 ， 48 （ D1 ）： D445 - D453 .

TURNBAUGH P J ， LEY R E ， MAHOWALD M A ， et al . An obesity-associated gut microbiome with increased capacity for energy harvest ［J］. Nature ， 2006 ， 444 （ 7122 ）： 1027 - 1031 .

KOLIADA A ， SYZENKO G ， MOSEIKO V ， et al . Association between body mass index and Firmicutes/Bacteroidetes ratio in an adult Ukrainian population ［J］. BMC Microbiol ， 2017 ， 17 （ 1 ）： 120 .

STOJANOV S ， BERLEC A ， ŠTRUKELJ B . The influence of probiotics on the Firmicutes/Bacteroidetes ratio in the treatment of obesity and inflammatory bowel disease ［J］. Microorganisms ， 2020 ， 8 （ 11 ）： 1715 .

SHAPIRO J M ， DE ZOETE M R ， PALM N W ， et al . Immunoglobulin A targets a unique subset of the microbiota in inflammatory bowel disease ［J］. Cell Host Microbe ， 2021 ， 29 （ 1 ）： 83 - 93 .

GUO W ， XIANG Q ， MAO B ， et al . Protective effects of microbiome-derived inosine on lipopolysaccharide-induced acute liver damage and inflammation in mice via mediating the TLR4/NF- κ B pathway ［J］. J Agric Food Chem ， 2021 ， 69 （ 27 ）： 7619 - 7628 .

DO M H ， LEE E ， OH M J ， et al . High-glucose or-fructose diet cause changes of the gut microbiota and metabolic disorders in mice without body weight change ［J］. Nutrients ， 2018 ， 10 （ 6 ）： 761 .

RASMUSSEN M . Aerococcus：An increasingly acknowledged human pathogen ［J］. Clin Microbiol Infect ， 2016 ， 22 （ 1 ）： 22 - 27 .

GILBERT N M ， CHOI B ， DU J ， et al . A mouse model displays host and bacterial strain differences in Aerococcus urinae urinary tract infection ［J］. Biol Open ， 2021 ， 10 （ 8 ）： bio058931 .

DOREY R B ， THEODOSIOU A A ， READ R C ， et al . The nonpathogenic commensal Neisseria： Friends and foes in infectious disease ［J］. Curr Opin Infect Dis ， 2019 ， 32 （ 5 ）： 490 - 496 .

D'ARGENIO V ， CASABURI G ， PRECONE V ， et al . Metagenomics reveals dysbiosis and a potentially pathogenic N. flavescens strain in Duodenum of adult celiac patients ［J］. Am J Gastroenterol ， 2016 ， 111 （ 6 ）： 879 - 890 .

YANG M C ， LING J ， MOSAED S . Capnocytophaga canimorsus blebitis：Case report and review of literature ［J］. BMC Ophthalmol ， 2021 ， 21 （ 1 ）： 59 .

LARSEN J M . The immune response to Prevotella bacteria in chronic inflammatory disease ［J］. Immunology ， 2017 ， 151 （ 4 ）： 363 - 374 .

KOTANI A ， ODA Y ， HIRAKAWA Y ， et al . Peritoneal dialysis-related peritonitis caused by Streptococcus oralis ［J］. Intern Med ， 2021 ， 60 （ 21 ）： 3447 - 3452 .

PITIROLLO O ， MICOLI F ， NECCHI F ， et al . Gold nanoparticles morphology does not affect the multivalent presentation and antibody recognition of group A Streptococcus synthetic oligorhamnans ［J］. Bioorg Chem ， 2020 ， 99 ： 103815 .

AGUIRRE M ， COLLINS M D . Lactic acid bacteria and human clinical infection ［J］. J Appl Bacteriol ， 1993 ， 75 （ 2 ）： 95 - 107 .

DERRIEN M ， VAN HYLCKAMA VLIEG J E . Fate， activity， and impact of ingested bacteria within the human gut microbiota ［J］. Trends Microbiol ， 2015 ， 23 （ 6 ）： 354 - 366 .

VEMURI R ， GUNDAMARAJU R ， SHINDE T ， et al . Lactobacillus acidophilus DDS-1 modulates intestinal-specific microbiota，short-chain fatty acid and immunological profiles in aging mice ［J］. Nutrients ， 2019 ， 11 （ 6 ）： 1297 .

ZHENG D ， WANG Z ， SUI L ， et al . Lactobacillus johnsonii activates porcine monocyte derived dendritic cells maturation to modulate Th cellular immune response ［J］. Cytokine ， 2021 ， 144 ： 155581 .

KONG C ， GAO R ， YAN X ， et al . Probiotics improve gut microbiota dysbiosis in obese mice fed a high-fat or high-sucrose diet ［J］. Nutrition ， 2019 ， 60 ： 175 - 184 .

PAGLIAI G ， RUSSO E ， NICCOLAI E ， et al . Influence of a 3-month low-calorie mediterranean diet compared to the vegetarian diet on human gut microbiota and SCFA：The CARDIVEG Study ［J］. Eur J Nutr ， 2020 ， 59 （ 5 ）： 2011 - 2024 .

THOMPSON C L ， VIER R ， MIKAELYAN A ， et al . ' Candidatus Arthromitus ' revised：Segmented filamentous bacteria in arthropod guts are members of Lachnospiraceae ［J］. Environ Microbiol ， 2012 ， 14 （ 6 ）： 1454 - 1465 .

HEDBLOM G A ， REILAND H A ， SYLTE M J ， et al . Segmented Filamentous Bacteria - Metabolism meets immunity ［J］. Front Microbiol ， 2018 ， 9 ： 1991 .

PAGOTTO F J ， NAZAROWEC-WHITE M ， BIDAWID S ， et al . Enterobacter sakazakii：Infectivity and enterotoxin production in vitro and in vivo ［J］. J Food Prot ， 2003 ， 66 （ 3 ）： 370 - 375 .

MANGE J P ， STEPHAN R ， BOREL N ， et al . Adhesive properties of enterobacter sakazakii to human epithelial and brain microvascular endothelial cells ［J］. BMC Microbiol ， 2006 ， 6 ： 58 .

TOWNSEND S M ， HURRELL E ， GONZALEZ-GOMEZ I ， et al . Enterobacter sakazakii invades brain capillary endothelial cells，persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat ［J］. Microbiology （Reading）， 2007 ， 153 （ Pt 10 ）： 3538 - 3547 .

IWASA S ， PETKANCHANOPONG W ， NAIGOWIT P ， et al . Endotoxin of Pseudomonas pseudomallei detected by the body weight-decreasing reaction in mice and comparison of it with those of P. cepacia and P. aeruginosa ［J］. JPN J Med Sci Biol ， 1992 ， 45 （ 1 ）： 35 - 47 .

ISEPPI R ， SABIA C ， BONDI M ， et al . Virulence factors，drug resistance and biofilm formation in pseudomonas species isolated from healthcare water systems ［J］. Curr Microbiol ， 2020 ， 77 （ 8 ）： 1737 - 1745 .

SUMI C D ， YANG B W ， YEO I C ， et al . Antimicrobial peptides of the genus Bacillus ： A new era for antibiotics ［J］. Can J Microbiol ， 2015 ， 61 （ 2 ）： 93 - 103 .

MCKILLIP J L . Prevalence and expression of enterotoxins in Bacillus cereus and other Bacillus spp.，a literature review ［J］. Antonie van Leeuwenhoek ， 2000 ， 77 （ 4 ）： 393 - 399 .

SARRABAYROUSE G ， LANDOLFI S ， POZUELO M ， et al . Mucosal microbial load in Crohn's disease： A potential predictor of response to faecal microbiota transplantation ［J］. EBio Med ， 2020 ， 51 ： 102611 .

SOKOL H ， BROT L ， STEFANESCU C ， et al . Prominence of ileal mucosa-associated microbiota to predict postoperative endoscopic recurrence in Crohn's disease ［J］. Gut ， 2020 ， 69 （ 3 ）： 462 - 472 .

李小萍 . 腹泻型肠易激综合征患者肠腔内菌群和肠道粘膜相关菌群的变化及意义［D］. 合肥：安徽医科大学， 2014 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Survival of Non-small Cell Lung Cancer Patients Treated with Fuzheng Gushe Therapy： A Real World Study

Chinese Medicine Monomers in Treatment of Osteosarcoma by Regulating PI3K/Akt/mTOR Signaling Pathway： A Review

Chinese Herbal Medicines Intervene in Signaling Pathways Associated with Diabetic Neuropathic Pain： A Review

Correlation Between Intestinal Flora and Traditional Chinese Medicine Syndromes of Diarrhea： A Review

Research Progress of Chinese Herbal Medicine for Softening Hardness to Dissipate Stagnation in Treatment of Malignant Tumors

Related Author

JIANG Min

LI Zhong

YANG Tao

HAO Lizheng

CHEN Jian

ZHU Xueying

LIU Kai

YANG Haodong

Related Institution

Beijing Shijitan Hospital，Capital Medical University

First Clinical Medical College， Beijing University of Chinese Medicine

Dongzhimen Hospital， Beijing University of Chinese Medicine

PLA Air Force Characteristic Medical Center

China Rehabilitation Research Center （Beijing Bo'ai Hospital）

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰