LI Zhuowei,LUO Liangzhong,LANG Jiaqi,et al.Effect of Organophosphate-Solubilizing Bacteria on Photosynthesis, Physiology, and Biochemistry of Paris polyphylla var. yunnanensis[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):165-171.
LI Zhuowei,LUO Liangzhong,LANG Jiaqi,et al.Effect of Organophosphate-Solubilizing Bacteria on Photosynthesis, Physiology, and Biochemistry of Paris polyphylla var. yunnanensis[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):165-171. DOI: 10.13422/j.cnki.syfjx.20220919.
Effect of Organophosphate-Solubilizing Bacteria on Photosynthesis, Physiology, and Biochemistry of Paris polyphylla var. yunnanensis
To study the effect of organophosphate-solubilizing bacteria and compound bacteria on the photosynthesis and physiological and biochemical characteristics of leaves of
Paris polyphylla
var.
yunnanensis
, and to provide a reference for selecting suitable bacterial fertilizers in artificial cultivation of this medicinal species.
Method
2
Pot experiment was carried out indoor and the following groups were designed: control (CK), inoculation with
Bacillus mycoides
(S1), inoculation with
B. wiedmannii
(S2), inoculation with
B. proteolyticus
(S3), inoculation with
B. mycoides
and
B. wiedmannii
(S4), inoculation with
B. mycoides
and
B. proteolyticus
(S5), inoculation with
B. wiedmannii
and
B. proteolyticus
(S6), and inoculation with
B. mycoides
,
B. wiedmannii
and
B. proteolyticus
(S7). Then, the growth and development, photosynthesis, and various physiological and biochemical indexes of the leaves of this species were observed.
Result
2
Compared with CK, the treatment groups showed decrease in content of malondialdehyde in the leaves (
P
<
0.05), particularly S7 (content was only about 1/3 that of the CK). The leaf area, photosynthetic parameters, photosynthetic pigment content, soluble sugar content, soluble protein content, and activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in leaves of the treatment groups were all improved. Among them, the leaf area, soluble sugar content, and soluble protein content were the highest in S7, which were 2.8, 2.1, and 2.2 times that of the CK, respectively. SOD activity peaked in S6 (2.9 times higher than that in the CK) and the highest activity of POD and CAT was detected in S5 (1.5 times and 2.1 times, respectively higher than that in the CK).
Conclusion
2
Inoculation with different organophosphate-solubilizing bacteria or compound bacteria can promote the growth and development of
P. polyphylla
var.
yunnanensis
and improve its resistance to stresses. The combination of
B. mycoides
and
B. proteolyticus
and the combination of the three achieved the have the best effect. This study provides a reference for the selection of bacterial fertilizers for artificial cultivation of
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Effect of Inoculation with Organophosphate Solubilizing Bacteria on Quality and Soil Fertility of Paris polyphylla var. yunnanensis
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Related Author
Zhuowei LI
Jiaqi LANG
Qi MENG
Mingyan YE
Jie WANG
Rujie SHI
Huihui DU
Nong ZHOU
Related Institution
College of Food and Biological Engineering,The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs, Chongqing Three Gorges University
Dexing Research and Training Center of Chinese Medical Sciences, Dexing
Cultivation Base of State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences
School of Traditional Chinese Medicine,Guangdong Pharmaceutical University
Northwest Collaborative Innovation Center of Traditional Chinese Medicine (TCM) and Tibetan Medicine, Gansu Key Laboratory Breeding Base for TCM Quality and Standard Research