LI Zhuowei,LANG Jiaqi,MENG Qi,et al.Effect of Inoculation with Organophosphate Solubilizing Bacteria on Quality and Soil Fertility of Paris polyphylla var. yunnanensis[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):156-164.
LI Zhuowei,LANG Jiaqi,MENG Qi,et al.Effect of Inoculation with Organophosphate Solubilizing Bacteria on Quality and Soil Fertility of Paris polyphylla var. yunnanensis[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(16):156-164. DOI: 10.13422/j.cnki.syfjx.20221018.
Effect of Inoculation with Organophosphate Solubilizing Bacteria on Quality and Soil Fertility of Paris polyphylla var. yunnanensis
The effect of inoculation with different organophosphate-resolving bacteria or compound bacteria on the quality of
Paris polyphylla
var.
yunnanensis
medicinal materials and rhizosphere soil fertility were studied to provide a reference for the development and application of biological bacterial fertilizer in artificial cultivation of
P. polyphylla
var.
yunnanensis
.
Method
2
The three dominant species of organophosphate-solubilizing bacteria were inoculated separately and in combination in sterilized soil by single-factor indoor pot planting, and no inoculation was used as the control group. The effect of inoculation of organophosphate-solubilizing bacteria on total saponins content in rhizomes of
P. polyphylla
var.
yunnanensis
, as well as microbial numbers, enzyme activities and nutrient contents in rhizosphere soil were analyzed.
Result
2
In the seven treatments inoculated with organophosphate-solubilizing bacteria, the total saponin content in the rhizomes of
Paris polyphylla
var.
yunnanensis
was increased by 16.42%, 3.83%, 16.86%, 33.69%, 2.11%, 13.44%, and 28.83%, respectively, compared with the control. Inoculation with organophosphate-solubilizing bacteria increased the number of soil microorganisms to varying degrees, and the effects of S6 and S7 treatments were the most significant. Inoculation with organophosphate-solubilizing bacteria improved the enzyme activity, and the effect of S7 treatment was the most significant. The activities of acid phosphatase, neutral phosphatase, alkaline phosphatase, protease, invertase and catalase were 49.96% and 104.67% , 110.17%, 99.61%, 26.26%, 11.29% higher than those of the control, respectively. Inoculation with organophosphate-solubilizing bacteria reduced the pH of the rhizosphere soil and increased the content of soil available nutrients. Under the S7 treatment, the contents of alkaline hydrolyzable nitrogen, available phosphorus and available potassium in the rhizosphere soil of
P. polyphylla
var.
yunnanensis
were 181.46%, 51.64% and 42.62% higher than those of the control, respectively. Correlation analysis showed that there was a significant positive correlation between total saponins and phosphatase activities, a significant positive correlation between soil microorganisms and soil enzyme activities, and a very significant positive correlation between soil nutrients.
Conclusion
2
Inoculation of different organophosphate-resolving bacteria or compound bacteria can improve the quality and rhizosphere soil fertility of
P. polyphylla
var.
yunnanensis
. Among them, the mixed inoculation of three kinds of bacteria and the mixed inoculation of
HAMEEDA B , HARINI G , RUPELA O P , et al . Growth promotion of maize by phosphate-solubilizing bacteria isolated from composts and macrofauna [J]. Microbiol Res , 2008 , 163 ( 2 ): 234 - 242 .
RAJ J , BAGYARAJ D J , MANJUNATH A . Infouence of soil inoculation with vesicular-arbuscular mycorrhiza and a phosphate dissolving bacterium on plant growth and 32p-up take [J]. Soil Biol Biochem , 1981 , 13 ( 1 ): 105 - 108 .
BASHAN Y , KAMNEV A A , DE-BASHAN L E . Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth:A proposal for an alternative procedure [J]. Biol Fertility Soils , 2013 , 49 ( 4 ): 465 - 479 .
BURNS R G , DEFOREST J L , MARXSEN J , et al . Soil enzymes in a changing environment:Current knowledge and future directions [J]. Soil Biol Biochem , 2013 , 58 : 216 - 234 .
Effect of Organophosphate-Solubilizing Bacteria on Photosynthesis, Physiology, and Biochemistry of Paris polyphylla var. yunnanensis
Effect of Different Combinations of Arbuscular Mycorrhiza on Paris polyphylla var. yunnanensis and Chemical Components
Effect of Different Arbuscular Mycorrhizal Fungi on Soil Nutrients in Rhizosphere Soil of Paris polyphylla var. yunnanensis Seedlings
Optimization Investigation of Extraction Technology of Rhizome Cimicifugae by Orthogonal Test with Multi-targets
Evaluation on Pre-Preparation of Total Saponins Extract from Bupleuri Radix
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