Chemical Constituents and Insulin Resistance Targets in Different Parts of<italic style="font-style: italic"> Morus alba</italic> by UPLC-Q-TOF-MS Combined with Network Pharmacology

WU Yuefeng; LIU Jinling; CHEN Dingfang; ZHU Zhifei; LIU Youzhi; YUAN Xiao; ZHOU Jin; HE Fuyuan

doi:10.13422/j.cnki.syfjx.20221214

您当前的位置：

首页 >

文章列表页 >

Chemical Constituents and Insulin Resistance Targets in Different Parts of Morus alba by UPLC-Q-TOF-MS Combined with Network Pharmacology

更新时间：2022-12-21

- Chemical Constituents and Insulin Resistance Targets in Different Parts of Morus alba by UPLC-Q-TOF-MS Combined with Network Pharmacology
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 2, Pages: 149-158(2023)
- 作者机构：
  
  湖南中医药大学药学院，中药成药性与制剂制备湖南省重点实验室，中医药超分子机理与数理特征化实验室，长沙 410208
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20221214
  CLC： R284.2;R285;R289;R22;R2-031;R33
- Received：04 March 2022，
  
  Published Online：12 July 2022，
  
  Published：20 January 2023
- 稿件说明：
移动端阅览
吴月峰,刘金玲,陈定芳等.基于UPLC-Q-TOF-MS联合网络药理学的桑源药材不同入药部位改善胰岛素抵抗的物质基础[J].中国实验方剂学杂志,2023,29(02):149-158.

WU Yuefeng,LIU Jinling,CHEN Dingfang,et al.Chemical Constituents and Insulin Resistance Targets in Different Parts of Morus alba by UPLC-Q-TOF-MS Combined with Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(02):149-158.
吴月峰,刘金玲,陈定芳等.基于UPLC-Q-TOF-MS联合网络药理学的桑源药材不同入药部位改善胰岛素抵抗的物质基础[J].中国实验方剂学杂志,2023,29(02):149-158. DOI： 10.13422/j.cnki.syfjx.20221214.

WU Yuefeng,LIU Jinling,CHEN Dingfang,et al.Chemical Constituents and Insulin Resistance Targets in Different Parts of Morus alba by UPLC-Q-TOF-MS Combined with Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(02):149-158. DOI： 10.13422/j.cnki.syfjx.20221214.

摘要

目的

基于液质联用联合网络药理学和分子对接技术比较桑源药材不同部位改善胰岛素抵抗（IR）物质基础异同。

方法

利用超高效液相色谱串联四极杆飞行时间质谱（UPLC-Q-TOF-MS）技术分析桑源药材不同部位的成分差异；用Sybyl-X2.1软件将成分与IR核心靶标进行对接，以Total Score≥5为遴选条件，绘制成分-疾病-靶点网络图；计算单靶点-成分对接评分数据集与总靶点-成分对接评分数据集的总量统计矩标准相似度（TQSMSS），筛选TQSMSS较高的靶点，构建蛋白质-蛋白质相互作用（PPI）网络，利用R语言对其进行基因本体论（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析。

结果

通过色谱-质谱技术共分析得到桑源药材的41种活性成分，总量统计矩（相似度）法筛选桑枝、桑白皮、桑椹、桑叶TQSMSS≥0.75的靶点数量分别为20、23、30、27，4味桑源药材中GO富集分析靶点功能排序为桑椹>桑叶>桑白皮>桑枝，涉及到血糖稳态、糖代谢过程、葡萄糖跨膜转运等生物过程；4味桑源药材KEGG通路富集排序为桑椹>桑枝>桑叶>桑白皮，涉及到单磷酸腺苷激活的蛋白激酶（AMPK）能量代谢信号通路、胰岛素调控相关的信号通路、抗炎及抗氧化应激的信号通路等。

结论

该研究表明了桑源药材不同部位改善IR的物质基础存在差异，为桑源药材不同部位的开发提供了依据。

Abstract

Objective

To compare the similarities and differences of material basis for improving insulin resistance （IR） in different parts of

Morus alba

based on liquid-mass combination combined with network pharmacology and molecular docking technology.

Method

Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS） was used to analyze the composition differences in different parts of

M. alba

. Sybyl-X2.1 was used to connect components with IR core targets， and the selection criterion was Total Score≥5. The "component-target-disease" network map was drawn. The total statistical moment standard similarity （TQSMSS） between the single target-component docking score data set and the total target-component docking score data set was calculated. The targets with higher TQSMSS were screened out， and the protein-protein interaction （PPI） network was constructed. The Gene Ontology （GO） functional analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analysis were performed using R language.

Result

Forty-one active components were obtained by UPLC-Q-TOF-MS. According to the total statistical moment （similarity） method， there were 20， 23， 30， and 27 targets with TQSMSS≥0.75 in Mori Ramulus， Mori Cortex， Mori Fructus， and Mori Folium， respectively. In the four

M. alba

medicinal sources， the functional order of the targets by GO enrichment analysis was Mori Fructus

Mori Folium

Mori Cortex

Mori Ramulus， which were involved in biological processes such as blood glucose homeostasis， glucose metabolism， and glucose transmembrane transport. The order of the four

M. alba

medicinal sources by KEGG pathway enrichment analysis was Mori Fructus

Mori Ramulus

Mori Folium

Mori Cortex， which were involved in the adenosine monophosphate-activated protein kinase （AMPK） energy metabolism signaling pathway， the insulin regulation-related signaling pathway， the anti-inflammatory and anti-oxidative stress signaling pathway， and so on.

Conclusion

This research demonstrates that there are differences in the material basis for improving IR by different parts of

M. alba

， which provides references for the development of different parts of

M. alba

关键词

Keywords

references

吴志平，周巧霞，顾振纶，等 . 桑树不同药用部位的降血糖效果比较［J］. 蚕业科学， 2005 ， 31 （ 2 ）： 215 - 217 .

史银春，傅强，王世东，等 . 国医大师吕仁和应用桑科植物治疗糖尿病及并发症临床经验［J］. 海南医学院学报， 2021 ， 27 （ 13 ）： 1028 - 1031 .

孙丰卉，王秋虹，邱宗林，等 . 中医药治疗2型糖尿病胰岛素抵抗的机制研究进展［J］. 医学综述， 2018 ， 24 （ 20 ）： 4068 - 4072，4077 .

李佳欣，陈思琦，葛鹏玲 . 2型糖尿病胰岛素抵抗研究进展［J］. 辽宁中医药大学学报， 2021 ， 23 （ 12 ）： 94 - 97 .

刘金玲，李文姣，王韧，等 . 基于UPLC-Q-TOF/MS技术的桑叶化学成分快速识别分析［J］. 中国中医药信息杂志， 2018 ， 25 （ 2 ）： 69 - 73 .

朱志飞，刘金玲，樊启猛，等 . 基于指纹图谱段带总量统计矩法和信息熵的桑源药材成分比较分析研究［J］. 中国中药杂志， 2021 ， 46 （ 10 ）： 2547 - 2555 .

吴月峰，朱志飞，刘金玲，等 . 基于分子连接性指数探讨桑源药材不同入药部位“异效”的物质基础［J］. 中国实验方剂学杂志， 2022 ， 28 （ 13 ）： 152 - 160 .

李媚，陈盛君，王协和，等 . 广藿香UPLC指纹图谱研究及基于网络药理学的广藿香潜在质量标志物预测［J］. 中草药， 2021 ， 52 （ 9 ）： 2665 - 2677 .

樊启猛，杨岩涛，肖美凤，等 . 基于分子对接技术的补阳还五汤成分与缺血性脑卒中靶点的相互作用研究［J］. 中草药， 2019 ， 50 （ 17 ）： 4200 - 4208 .

牛丽华，于丰彦，周福生 . 胰岛素抵抗的中医病机［J］. 中国医药指南， 2008 ， 6 （ 24 ）： 383 - 384 .

徐芹庭 . 细说黄帝内经［M］. 北京：新世界出版社， 2007 ： 124 .

吴月峰，陈定芳，朱志飞，等 . 桑源中药用药规律及超分子作用机制研究［J］. 湖南中医药大学学报， 2021 ， 41 （ 11 ）： 1807 - 1812 .

KUMAR S ， SINHA K ， SHARMA R ， et al . Phloretin and phloridzin improve insulin sensitivity and enhance glucose uptake by subverting PPAR γ /Cdk5 interaction in differentiated adipocytes ［J］. Exp Cell Res ， 2019 ， 383 （ 1 ）： 111480 .

AHN E M ， ASAMENEW G ， KIM H W ， et al . Anti-obesity effects of Petasites japonicus （Meowi） ethanol extract on raw 264.7 macrophages and 3T3-L1 adipocytes and its characterization of polyphenolic compounds ［J］. Nutrients ， 2020 ， 12 （ 5 ）： 1261 .

PAUDEL P ， YU T ， SEONG S ， et al . Protein tyrosine phosphatase 1B inhibition and glucose uptake potentials of mulberrofuran G， albanol B， and kuwanon G from root bark of Morus alba L. in insulin-resistant HepG2 cells： An in vitro and in silico study ［J］. Int J Mol Sci ， 2018 ， 19 （ 5 ）： 1542 .

KIM H B ， GO E J ， RYU B R ， et al . Anti-obesity effect of mulberry anthocyanins in C57BL/6J mice ［J］. Korean J Medicinal Crop Sci ， 2021 ， 29 （ 5 ）： 317 - 327 .

WU T ， QI X ， LIU Y ， et al . Dietary supplementation with purified mulberry （ Morus australis Poir） anthocyanins suppresses body weight gain in high-fat diet fed C57BL/6 mice ［J］. Food Chem ， 2013 ， 141 （ 1 ）： 482 - 487 .

KHLIFI R ， DHAOUEFI Z ， TOUMIA I B ， et al . Erica multiflora extract rich in quercetin-3- O -glucoside and kaempferol-3- O -glucoside alleviates high fat and fructose diet-induced fatty liver disease by modulating metabolic and inflammatory pathways in Wistar rats ［J］. J Nutr Biochem ， 2020 ， 86 ： 108490 .

HUANG Y ， ZHANG X . Luteolin alleviates polycystic ovary syndrome in rats by resolving insulin resistance and oxidative stress ［J］. Am J Physiol Endocrinol Metab ， 2021 ， 320 （ 6 ）： E1085 - E1092 .

KWON E ， KIM S Y ， CHOI M . Luteolin-enriched artichoke leaf extract alleviates the metabolic syndrome in mice with high-fat diet-induced obesity ［J］. Nutrients ， 2018 ， 10 （ 8 ）： 979 .

LI J S ， JI T ， SU S L ， et al . Mulberry leaves ameliorate diabetes via regulating metabolic profiling and AGEs/RAGE and p38 MAPK/NF- κ B pathway ［J］. J Ethnopharmacol ， 2022 ， 283 ： 114713 .

贺福元，邓凯文，黄胜，等 . 总量统计矩标准相似度数学模型的建立及应用研究［J］. 药学学报， 2013 ， 48 （ 9 ）： 1453 - 1458 .

YANG Y ， ZHOU T ， LIU Y ， et al . Identification of HNF4A mutation p.T130I and HNF1A mutations p.I27L and p.S487N in a Han Chinese family with early-onset maternally inherited type 2 diabetes ［J］. J Diabetes Res ， 2016 ， 2016 ： 3582616 .

SETHI J K ， HOTAMISLIGIL G S . Metabolic messengers：Tumour necrosis factor ［J］. Nat Metab ， 2021 ， 3 （ 10 ）： 1302 - 1312 .

李寒，王宁，马伯艳 . 基于肝细胞焦亡的黄连温胆汤干预IGT IR的机制［J］. 中国中药杂志， 2021 ， 46 （ 13 ）： 3394 - 3401 .

朱大龙，陆菊明 . 《中国2型糖尿病防治指南（2020年版）》编写说明［J］. 中华糖尿病杂志， 2021 ， 13 （ 4 ）： 312 - 314 .

CHIEFARI E ， FOTI D P ， SGARRA R ， et al . Transcriptional regulation of glucose metabolism： The emerging role of the HMGA1 chromatin factor ［J］. Front Endocrinol （Lausanne）， 2018 ， 9 ： 357 .

QIU F ， WANG J ， LIU H Y ， et al . Mulberry bark alleviates effect of STZ inducing diabetic mice through negatively regulating FoxO1 ［J］. Evid Based Complement Alternat Med ， 2019 ， 2019 ： 2182865 .

LIU H Y ， FANG M ， ZHANG Y Q . In vivo hypoglycaemic effect and inhibitory mechanism of the branch bark extract of the mulberry on STZ-induced diabetic mice ［J］. Sci World J ， 2014 ， 2014 ： 614265 .

YOU S ， JANG M ， KIM G . Mori Cortex radicisattenuates high fat diet-induced cognitive impairment via an IRS/Akt signaling pathway ［J］. Nutrients ， 2020 ， 12 （ 6 ）： 1851 .

YAN F ， ZHENG X . Anthocyanin-rich mulberry fruit improves insulin resistance and protects hepatocytes against oxidative stress during hyperglycemia by regulating AMPK/ACC/mTOR pathway ［J］. J Funct Foods ， 2017 ， 30 ： 270 - 281 .

CHOI K H ， LEE H A ， PARK M H ， et al . Mulberry （ Morus alba L.） Fruit extract containing anthocyanins improves glycemic control and insulin sensitivity via activation of AMP-activated protein kinase in diabetic C57BL/Ksj-db/db Mice ［J］. J Med Food ， 2016 ， 19 （ 8 ）： 737 - 745 .

CAI S ， SUN W ， FAN Y ， et al . Effect of mulberry leaf （Folium Mori） on insulin resistance via IRS-1/PI3K/Glut-4 signalling pathway in type 2 diabetes mellitus rats ［J］. Pharm Biol ， 2016 ， 54 （ 11 ）： 2685 - 2691 .

贺福元，邓凯文，刘文龙，等 . 中药复方对人体作用本质：“网通虹势”的多重遗传谱效动力学［J］. 中国实验方剂学杂志， 2011 ， 17 （ 2 ）： 240 - 247 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Herbal Textual Research on Mori in Famous Classical Formulas

Analysis on Changes of Chemical Composition Contents and Efficacy of Improving Insulin Resistance in Type 2 Diabetic Mice of Anemarrhenae Rhizoma Before and After Processing with Salt-water

Investigate Material Foundation of Puerariae Radix for Improving Insulin Resistance Based on Molecular Docking Technology

Mahoniae Caulis Alkaloids Ameliorate Depression by Regulating Synaptic Plasticity via cAMP Pathway

Analysis of Potential Active Components and Molecular Mechanism of Baoxin Granules Regulating Ferroptosis in Treatment of Heart Failure

Related Author

Wen-min DU

Zhi-lai ZHAN

Hui JIANG

Zhao-yong ZHOU

Jing-qiong WAN

Tian-yue LIAO

Zhen OUYANG

Wei ZHENG

Related Institution

School of Pharmacy，School of Food and Biological Engineering， Jiangsu University

National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

（Chinese Materia Medica Processing Engineering and Technology Center of Liaoning Province， Key Laboratory of Processing Principle Analysis，National Administration of Traditional Chinese Medicine （TCM），School of Pharmacy，Liaoning University of TCM，，）

Key Laboratory of Chemistry and Engineering of Forest Products， National Ethnic Affairs Commission， Guangxi Key Laboratory of Chemistry and Engineering of Forest Products/Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products， School of Chemistry and Chemical Engineering， Guangxi Minzu University

Guangxi Key Laboratory of Chinese Medicine Quality Standard， Guangxi Institute of Chinese Medicine and Pharmaceutical Science

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Chemical Constituents and Insulin Resistance Targets in Different Parts of Morus alba by UPLC-Q-TOF-MS Combined with Network Pharmacology

DOI：10.13422/j.cnki.syfjx.20221214

摘要

Abstract

关键词

Keywords

references