Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of <italic style="font-style: italic">Angelica sinensis </italic>Based on Hybrid Sequencing Strategy

XU Li; ZHU Tiantian; JIN Ling; KANG Shuqi; ZHANG Minghui; WANG Yuanyuan

doi:10.13422/j.cnki.syfjx.20221514

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Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of Angelica sinensis Based on Hybrid Sequencing Strategy

更新时间：2022-12-08

- Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of Angelica sinensis Based on Hybrid Sequencing Strategy
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 1, Pages: 141-147(2023)
- 作者机构：
  
  1.甘肃中医药大学，兰州 730000
  2.西北中藏药省部共建协同创新中心，兰州 730000
  3.甘肃省珍稀中药资源评价与保护利用工程研究中心，兰州 730000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20221514
  CLC： R284.2;R289;R22;R2-031;R33
- Received：03 May 2022，
  
  Published Online：15 September 2022，
  
  Published：05 January 2023
- 稿件说明：
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徐丽,朱田田,晋玲等.基于混合测序策略的两品种当归中类黄酮调控基因差异分析[J].中国实验方剂学杂志,2023,29(01):141-147.

XU Li,ZHU Tiantian,JIN Ling,et al.Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of Angelica sinensis Based on Hybrid Sequencing Strategy[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(01):141-147.
徐丽,朱田田,晋玲等.基于混合测序策略的两品种当归中类黄酮调控基因差异分析[J].中国实验方剂学杂志,2023,29(01):141-147. DOI： 10.13422/j.cnki.syfjx.20221514.

XU Li,ZHU Tiantian,JIN Ling,et al.Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of Angelica sinensis Based on Hybrid Sequencing Strategy[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(01):141-147. DOI： 10.13422/j.cnki.syfjx.20221514.

摘要

目的

比较2个不同茎叶色当归品种岷归1号与岷归2号转录水平差异。

方法

以2种颜色当归的新鲜叶片（带叶柄）和上端茎为材料，采用混合测序策略，应用全长转录组技术构建当归无参全长转录本文库，利用RNA-seq技术对两品种进行差异基因表达分析，再利用公共数据库对差异基因的生物学功能进行注释和精细分类，筛选调控当归茎叶色差异的主要候选基因。

结果

当归转录本的测序结果良好，测序数据质量较高，当归全长转录本的34 528条序列在非冗余蛋白（NR）、京都基因与基因组百科全书（KEGG）、SwissProt及KOG数据库中分别注释到33 947、33 241、29 150和22 601条。对两品种当归差异表达基因（DEGs）进行精细分类，具有生物学功能和分子功能的705个DGEs可分为11类，主要富集在初级代谢（17.87%）、逆境响应（14.47%）、次级代谢（11.49%）等功能上，与颜色有关的差异表达基因主要集中在类黄酮生物合成途径上。

结论

两品种当归茎叶颜色差异的主要原因可能与调控类黄酮的生物合成基因的表达差异有关，可为后续进行功能验证，进一步明确与当归主要药效成分之间的联系奠定基础。

Abstract

Objective

To compare the transcriptional levels of two cultivars （Mingui 1 and Mingui 2） with different stem and leaf colors.

Method

The fresh leaves with petioles and the upper stems from

Angelica sinensis

with two colors were selected as the material， and the hybrid sequencing strategy was used. The technology at the transcriptome was used to build the non-reference full-length transcript library of

A. sinensis

， and the RNA-seq technology was used to analyze differentially expressed genes of the two cultivars， reusing a public database for biology function annotation and fine classification of differentially expressed genes. The main candidate genes regulating color differences between stems and leaves of

A. sinensis

were screened out.

Result

The sequencing results of

A. sinensis

transcripts were good and the quality of the sequencing data was high. The 34 528 full-length transcripts were annotated into 33 947， 33 241， 29 150， and 22 601 in the Non-redundant Protein Sequence Database （NR）， Kyoto Encyclopedia of Genes and Genomes （KEGG）， SwissProt， and Clusters of Orthologous Groups for Eukaryotic Complete Genomes （KOG）， respectively. The 705 differentially expressed genes of the two cultivars with biological and molecular functions were divided into 11 categories， which were mainly enriched in the primary metabolism （17.87%）， stress response （14.47%）， and secondary metabolism （11.49%）. The differentially expressed genes related to colors were mainly concentrated in the flavonoid biosynthesis pathway.

Conclusion

The main reason for the color differences of the stems and leaves in two cultivars of

A. sinensis

may be related to the expression differences of genes regulating flavonoid biosynthesis， which lays a foundation for subsequent functional verification and further clarification of the relationship with the main pharmacodynamic components of

A. sinensis.

关键词

Keywords

references

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Differential Analysis of Flavonoid-regulated Genes in Two Cultivars of Angelica sinensis Based on Hybrid Sequencing Strategy

DOI：10.13422/j.cnki.syfjx.20221514

摘要

Abstract

关键词

Keywords

references