LUO Yingzi,HUANG Mingjin,WANG Dachang,et al.Microbial Diversity in Rhizosphere Soil of Gastrodia elata with Different Yields[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(01):133-140.
LUO Yingzi,HUANG Mingjin,WANG Dachang,et al.Microbial Diversity in Rhizosphere Soil of Gastrodia elata with Different Yields[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(01):133-140. DOI: 10.13422/j.cnki.syfjx.20221515.
Microbial Diversity in Rhizosphere Soil of Gastrodia elata with Different Yields增强出版
To analyze the microbial diversity in the rhizosphere soil of
Gastrodia elata
with different yields and explore the influence of soil microorganisms on the yield of
G. elata
.
Method
2
The experiment adopted the 16S DNA and ITS high-throughput sequencing technologies to study the diversity of the bacterial and fungal community in the rhizosphere soil of
G. elata
with high yield (GC) and low yield (DC).
Result
2
Proteobacteria, Firmicutes, and other unidentified Bacteria were dominant in the rhizosphere soil of
G. elata
. The dominant rhizosphere fungi were
A
scomycota, Basidiomycota, and Mortierellomycota. There was no significant difference in microbial community abundance in the high-yield and low-yield rhizosphere soil of
G. elata
, but there was a significant difference in species composition. Thirty-eight microbes such as
Bradyrhizobium
,
Schleiferilactobacillus
, and
Archaeorhizomyces
were gathered in large numbers in the high-yield rhizosphere soil, and thirty microbes such as
Fusarium
,
Coprinellus
, and
Nitrosotalea
were gathered in large numbers in the low-yield rhizosphere soil. At the level of genus and species, there were six different species in the high-yield and low-yield rhizosphere soil of
G. elata
, among which
Russula mariae
,
Archeaeorhizomyces
, and
Ilyonectria
were gathered in the high-yield rhizosphere soil of
G. elata
, while
Nitrosotalea
,
Coprinellus disserminatus
, and
Fusarium
were gathered in the low-yield rhizosphere soil of
G. elata
.
Conclusion
2
There are different microorganisms in the rhizosphere soil of
G. elata
with different yields, and it is speculated that these microorganisms are related to the yields of
G. elata
. The research results are expected to provide a vital theoretical basis for the follow-up study of the high yield of
MAÑERO F J , ALGAR E , MARTÍN GÓMEZ M S , et al . Elicitation of secondary metabolism in Hypericum perforatum by rhizosphere bacteria and derived elicitors in seedlings and shoot cultures [J]. Pharm Biol , 2012 , 50 ( 10 ): 1201 - 1209 .
LIU T , HUA Z , HAN P , et al . Mycorrhizosphere Bacteria , Rahnella sp. HPDA25, promotes the growth of Armillaria gallica and its parasitic host Gastrodia elata [J]. Front Microbiol , 2022 , 13 : 842893 .
CHIARINI L B A , DALMASTRI C , CHIONI S , et al . Influence of plantdevelopment,cultivar and soil type on microbial colonization of maizeroot [J]. Applied Soil Ecology , 1998 , 8 : 11 - 18 .
DURÁN P , THIERGART T , GARRIDO-OTER R , et al . Microbial interkingdom interactions in roots promote Arabidopsis survival [J]. Cell , 2018 , 175 ( 4 ): 973 - 983 .
XIA L , SZLAVECZ K , SWAN C M . Inter- and intra-specific interactions of Lumbricus rubellus (Hoffmeister, 1843) and Octolasion lacteum (Örley, 1881) (Lumbricidae) and the implication for C cycling [J]. Soil Biol Biochem , 2011 , 43 ( 7 ): 1584 - 1590 .
MEENA R S , VIJAYAKUMAR V , AL E . Response and interaction of Bradyrhizobium japonicum and arbuscular mycorrhizal fungi in the soybean rhizosphere [J]. Plant Growth Regulation , 2017 , 84 ( 2 ): 207 - 223 .
NAIK P R , RAMAN G , NARAYANAN K B , et al . Assessment of genetic and functional diversity of phosphate solubilizing fluorescent pseudomonads isolated from rhizospheric soil [J]. BMC Microbiol , 2008 , 8 : 230 .
TRAN H , KRUIJT M , RAAIJMAKERS J M . Diversity and activity of biosurfactant-producing Pseudomonas in the rhizosphere of black pepper in vietnam [J]. J Appl Microbiol , 2008 , 104 ( 3 ): 839 - 851 .
MALANDRAKI I , TJAMOS S E , AL E . Thermal inactivation of compost suppressiveness implicates possible biological factors in disease management [J]. Biological Control , 2008 , 44 ( 2 ): 180 - 187 .