Mechanism of Radix Paeoniae Rubra-Radix Aconiti Lateralis in Treatment of Acute-on-chronic Liver Failure Based on Macrophage Polarization

Nianhua TAN; Yunan CAO; Chenqin TANG; Jie PENG; Bin CHEN

doi:10.13422/j.cnki.syfjx.20221595

您当前的位置：

首页 >

文章列表页 >

Mechanism of Radix Paeoniae Rubra-Radix Aconiti Lateralis in Treatment of Acute-on-chronic Liver Failure Based on Macrophage Polarization

更新时间：2022-06-28

- Mechanism of Radix Paeoniae Rubra-Radix Aconiti Lateralis in Treatment of Acute-on-chronic Liver Failure Based on Macrophage Polarization
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 15, Pages: 35-41(2022)
- 作者机构：
  
  1.湖南中医药大学，长沙 410208
  2.湖南中医药大学第一附属医院，长沙 410007
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20221595
  CLC： R242;R285.5;R2-031;R966;R256.4;R575.3
- Received：22 December 2021，
  
  Published Online：22 March 2022，
  
  Published：05 August 2022
- 稿件说明：
移动端阅览
谭年花,曹钰楠,唐陈琴等.基于巨噬细胞极化研究“赤芍-附片”治疗慢加急性肝衰竭的作用机制[J].中国实验方剂学杂志,2022,28(15):35-41.

TAN Nianhua,CAO Yunan,TANG Chenqin,et al.Mechanism of Radix Paeoniae Rubra-Radix Aconiti Lateralis in Treatment of Acute-on-chronic Liver Failure Based on Macrophage Polarization[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(15):35-41.
谭年花,曹钰楠,唐陈琴等.基于巨噬细胞极化研究“赤芍-附片”治疗慢加急性肝衰竭的作用机制[J].中国实验方剂学杂志,2022,28(15):35-41. DOI： 10.13422/j.cnki.syfjx.20221595.

TAN Nianhua,CAO Yunan,TANG Chenqin,et al.Mechanism of Radix Paeoniae Rubra-Radix Aconiti Lateralis in Treatment of Acute-on-chronic Liver Failure Based on Macrophage Polarization[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(15):35-41. DOI： 10.13422/j.cnki.syfjx.20221595.

摘要

目的

研究赤芍-附片对慢加急性肝衰竭（ACLF）大鼠的治疗作用及其对M1/M2型巨噬细胞极化的影响。

方法

将SD雄性大鼠随机分为正常组、模型组、乳果糖组（乳果糖 1.8 g·kg

-1

）、中药组（赤芍-附片 5.85 g·kg

-1

），每组6只。采用牛血清白蛋白皮下及尾静脉注射联合腹腔注射

-半乳糖+脂多糖急性攻击建立ACLF模型，并予以相应药物灌胃1周，空白组和模型组予以蒸馏水灌胃。利用苏木素-伊红（HE）染色观察肝组织病理变化，实时荧光定量聚合酶链式反应（Real-time PCR）、蛋白免疫印迹法（Western blot）、免疫组化等方法比较各组大鼠肝组织CD86、诱导型一氧化氮合酶（iNOS）、CD206、精氨酸酶1（Arg1） mRNA和蛋白表达水平。

结果

与正常组比较，模型组大鼠肝组织假小叶形成，肝细胞形态变化、坏死，伴大量炎性细胞浸润，CD86、iNOS mRNA及蛋白表达均显著上调（

0.01）；与模型组比较，各给药组肝组织坏死及炎性浸润均改善，CD86、iNOS mRNA及蛋白表达下调（

0.01），而CD206、Arg1 mRNA及蛋白表达上调（

0.05，

0.01），与乳果糖组比较，中药组上调CD206、Arg1作用更优（

0.01）。

结论

ACLF大鼠存在M1/M2型巨噬细胞极化失衡，失衡向M1方向偏移，赤芍-附片通过促进肝脏巨噬细胞向M2方向极化，抑制M1型巨噬细胞活化，减轻肝衰竭炎症反应。

Abstract

Objective

To observe the therapeutic effect of Radix Paeoniae Rubra-Radix Aconiti Lateralis on acute-on-chronic liver failure （ACLF） rats and its effect on M1/M2 macrophage polarization.

Method

Male SD rats were randomly divided into normal group， model group， positive group （lactulose， 1.8 g·kg

-1

） and traditional Chinese medicine （TCM） group （Radix Paeoniae Rubra-Radix Aconiti Lateralis， 5.85 g·kg

-1

）， six in each group. The ACLF rat model was established by subcutaneous and tail vein injection of bovine serum albumin combined with intraperitoneal injection of

-galactosamine+lipopolysaccharide. Then the modeled rats were intervened with corresponding drugs for one week. The normal group and model group were given the same dose of distilled water. The histopathological changes of liver tissue were observed by hematoxylin-eosin （HE） staining. The mRNA and protein expression levels of CD86， inducible nitric oxide synthase （iNOS）， CD206 and arginase 1 （Arg1） were detected by real-time polymerase chain reaction （Real-time PCR）， Western blot and immunohistochemistry.

Result

Compared with the conditions in the normal group， pseudolobule formation in liver tissue and morphological changes and necrosis of hepatocytes were observed in ACLF rats， accompanied by a large number of inflammatory cell infiltration. Moreover， the mRNA and protein expression levels of CD86， iNOS were up-regulated（

0.01）. Compared with the model group， the treatment groups had improved necrosis and inflammatory infiltration of hepatocytes， down-regulated mRNA and protein expression of CD86 and iNOS （

0.01） and up-regulated mRNA and protein expression of CD206 and Arg1 （

0.05，

0.01）， with the up regulation in the TCM group better than that in the positive group.

Conclusion

ACLF rats had unbalanced M1/M2 macrophage polarization， and the imbalance shifted towards M1. Radix Paeoniae Rubra-Radix Aconiti Lateralis inhibited the activation of M1 macrophages and reduced the inflammatory response of liver failure by promoting the polarization of liver macrophages towards M2.

关键词

Keywords

references

中华医学会感染病学分会肝衰竭与人工肝学组，中华医学会肝病学分会重型肝病与人工肝学组 . 肝衰竭诊治指南（2018年版）［J］. 临床肝胆病杂志， 2019 ， 35 （ 1 ）： 38 - 44 .

SARIN S K ， CHOUDHURY A . Acute-on-chronic liver failure：Terminology，mechanisms and management ［J］. Nat Rev Gastroenterol Hepatol ， 2016 ， 13 （ 3 ）： 131 - 149 .

LI Q ， WANG J ， LU M ， et al . Acute-on-chronic liver failure from chronic-hepatitis-B，who is the behind scenes ［J］. Front Microbiol ， 2020 ， 11 ： 583423 .

LI L ， ZENG Z . Live imaging of innate and adaptive immune responses in the liver ［J］. Front Immunol ， 2020 ， 11 ： 564768 .

周顺 . 巨噬细胞免疫应答调控在急性肝损伤中的作用及机制研究［D］. 南京：南京医科大学， 2019 .

NIELSEN M C ， HVIDBJERG GANTZEL R ， CLÀRIA J ， et al . Macrophage activation markers，CD163 and CD206，in acute-on-chronic liver failure ［J］. Cells ， 2020 ， 9 （ 5 ）： 1175 .

陈斌，丁秀丽，彭杰，等 . 清温并用法对慢加急性肝衰竭肠源性内毒素血症大鼠结肠组织FoxP3，ROR- γ t表达的影响［J］. 中国实验方剂学杂志， 2020 ， 26 （ 2 ）： 19 - 25 .

黄裕红，陈斌 . 重用赤芍、附子治疗慢性重型乙型肝炎98例［J］. 中国中医急症， 2010 ， 19 （ 6 ）： 1030 - 1031 .

中国中西医结合学会 . 慢加急性肝衰竭中西医结合诊疗专家共识［J］. 临床肝胆病杂志， 2021 ， 37 （ 9 ）： 2045 - 2053 .

彭杰，陈斌，孙克伟，等 . 慢性乙型重型肝炎"湿热-血瘀-脾虚"证候分布与演变特点的回顾性分析［J］. 中西医结合肝病杂志， 2011 ， 21 （ 3 ）： 135 - 138 .

黄湛镰，高志良 . 肝衰竭的三重打击及治疗策略［J］. 内科急危重症杂志， 2014 ， 20 （ 3 ）： 154 - 156 .

KRENKEL O ， TACKE F . Liver macrophages in tissue homeostasis and disease ［J］. Nat Rev Immunol ， 2017 ， 17 （ 5 ）： 306 - 321 .

张耿林，高志良 . 探索乙型肝炎相关慢加急性肝衰竭免疫调节治疗的新思路［J］. 中国病毒病杂志， 2019 ， 9 （ 1 ）： 1 - 5 .

YUNNA C ， MENGRU H ， LEI W ， et al . Macrophage M1/M2 polarization ［J］. Eur J Pharmacol ， 2020 ， 877 ： 173090 .

XIE Y ， ZHAO D ， DONG P ， et al . Macrophage-targeting Fasudil treatment protects liver from the ischemia/reperfusion injury by promoting M2 macrophage polarization ［J］. Biosci Rep ， 2018 ， doi： 10.1042/BSR20171734 http://dx.doi.org/10.1042/BSR20171734 .

ZHOU S ， GU J ， LIU R ， et al . Spermine alleviates acute liver injury by inhibiting liver-resident macrophage pro-inflammatory response through ATG5-dependent autophagy ［J］. Front Immunol ， 2018 ， 9 ： 948 .

阳乔 . 单核/巨噬细胞在肝衰竭中的作用及调节机制研究［D］. 杭州：浙江大学， 2013 .

刘刚，江宇，陈迎晓 . 口服乳果糖治疗急性及亚急性肝衰竭的疗效观察与机制探讨［J］. 保健医学研究与实践， 2014 ， 11 （ 2 ）： 34 - 36 .

KHANAM A ， KOTTILIL S . Abnormal innate immunity in acute-on-chronic liver failure：immunotargets for therapeutics ［J］. Front Immunol ， 2020 ， 11 ： 2013 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Xuefu Zhuyu Capsules on Polarization of Macrophages in Mice with Atherosclerosis

Effect of Combined Therapy of Lung and Intestine on NF-κB Inflammatory Pathway and Macrophage Polarization in LPS-induced Acute Lung Injury Rats

Exploring Mechanism of Kelong Capsules in Treatment of Benign Prostatic Hyperplasia Based on Nrf2 Pathway and Macrophage Activation

Shaoyaotang Regulates Glucose Metabolism Reprogramming to Inhibit Macrophage Polarization Toward M1 Phenotype

Related Author

LIU Menghua

ZHANG Dongmei

CHENG Xu

ZHAO Mengzhu

WEI Qiong

WANG Rui-zhe

KOU Yu-le

HE Hong-wei

Related Institution

Dongzhimen Hospital， Beijing University of Chinese Medicine

Affiliated Hospital，Shaanxi University of Chinese Medicine

College of Basic Medicine，Shaanxi University of Chinese Medicine

The First Clinical Medical College of Shaanxi University of Chinese Medicine

Experimental Research Center，China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰