Shaoyaotang Alleviates Ulcerative Colitis by Regulating Th17/Treg Balance Through IL-6/STAT3 Signaling Pathway

WU Dongsheng; CAO Hui; ZHANG Yu; ZOU Bo; HU Qihua; XIE Nianjia; YANG Yuting; LU Yi

doi:10.13422/j.cnki.syfjx.20221602

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Shaoyaotang Alleviates Ulcerative Colitis by Regulating Th17/Treg Balance Through IL-6/STAT3 Signaling Pathway

更新时间：2023-03-30

- Shaoyaotang Alleviates Ulcerative Colitis by Regulating Th17/Treg Balance Through IL-6/STAT3 Signaling Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 9, Pages: 46-52(2023)
- 作者机构：
  
  1.湖南中医药大学第一附属医院，长沙 410007
  2.湖南中医药大学，长沙 410208
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20221602
  CLC： R2-0;R22;R285.5;R289;R33
- Published：05 May 2023，
  
  Published Online：03 August 2022，
  
  Received：31 May 2022，
- 稿件说明：
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吴东升,曹晖,张彧等.基于IL-6/STAT3通路探讨芍药汤对溃疡性结肠炎Th17/Treg平衡的调节机制[J].中国实验方剂学杂志,2023,29(09):46-52.

WU Dongsheng,CAO Hui,ZHANG Yu,et al.Shaoyaotang Alleviates Ulcerative Colitis by Regulating Th17/Treg Balance Through IL-6/STAT3 Signaling Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(09):46-52.
吴东升,曹晖,张彧等.基于IL-6/STAT3通路探讨芍药汤对溃疡性结肠炎Th17/Treg平衡的调节机制[J].中国实验方剂学杂志,2023,29(09):46-52. DOI： 10.13422/j.cnki.syfjx.20221602.

WU Dongsheng,CAO Hui,ZHANG Yu,et al.Shaoyaotang Alleviates Ulcerative Colitis by Regulating Th17/Treg Balance Through IL-6/STAT3 Signaling Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(09):46-52. DOI： 10.13422/j.cnki.syfjx.20221602.

摘要

目的

探讨白细胞介素-6（IL-6）/信号传导及转录激活因子3（STAT3）通路在湿热内蕴证溃疡性结肠炎（UC）辅助性T细胞17（Th17）/调节性T细胞（Treg）失衡中的作用及芍药汤的干预机制。

方法

将60只SD大鼠随机分为空白组、模型组、美沙拉嗪组（0.42 g·kg

-1

）、芍药汤低、中、高剂量组（11.1、22.2、44.4 g·kg

-1

），除空白组外，其他各组采用复合病因造模建立湿热内蕴证溃疡性结肠炎模型，各组分别给予生理盐水、美沙拉嗪组、芍药汤低、中、高剂量治疗14 d。末次给药24 h后处死大鼠提取脾脏、结肠组织，采用苏木素-伊红（HE）染色观察结肠组织病理学改变，免疫组化（IHC）法检测结肠组织白细胞介素-17（IL-17）、转化生长因子-

（TGF-

）水平；流式细胞术检测脾脏Th17/Treg细胞水平；蛋白免疫印迹法（Western blot）检测结肠组织IL-6、STAT3蛋白水平。

结果

与空白组比较，模型组结肠出现充血、糜烂等病变，脾Treg细胞百分比显著降低（

0.01），Th17细胞百分比显著升高（

0.01），结肠组织IL-6、STAT3蛋白水平显著升高（

0.01）；与模型组比较，各药物组结肠损伤减轻，脾Treg细胞百分比显著升高（

0.01），Th17细胞百分比显著降低（

0.01），结肠组织中IL-6、STAT3蛋白水平显著降低（

0.01）。

结论

芍药汤通过抑制IL-6/STAT3信号通路调节Th17/Treg平衡，进而改善湿热内蕴证UC大鼠的病理损伤，影响其免疫功能。

Abstract

Objective

To explore the role of interleukin-6 （IL-6）/signal transducer and activator of transcription 3 （STAT3） pathway in the balance of T helper 17 （Th17）/regulatory T （Treg） cells in ulcerative colitis （UC） with internal dampness-heat accumulation syndrome and the intervention mechanism of Shaoyaotang.

Method

A total of 60 SD rats were randomized into blank group （equivalent volume of normal saline）， model group （equivalent volume of normal saline）， western medicine control group （0.42 g·kg

-1

mesalazine）， and low-dose （11.1 g·kg

-1

）， medium-dose （22.2 g·kg

-1

）， and high-dose （44.4 g·kg

-1

） Shaoyaotang groups. UC with internal dampness-heat accumulation syndrome was induced in rats with the compound method except for the blank group. The administration lasted 14 days for each group. At 24 h after the last administration， rats were killed and the spleen and colon tissues were separated. The histopathological changes of colon were observed based on hematoxylin and eosin （HE） staining and the levels of interleukin-17 （IL-17） and transforming growth factor-

（TGF-

） in colon tissue were detected by immunohistochemistry （IHC）. Flow cytometry was employed to determine the levels of Th17/Treg cells in the spleen， and Western blot to measure the levels of IL-6 and STAT3 proteins in colon tissue.

Result

Compared with the blank group， the model group had lesions such as congestion and erosion， low percentage of spleen Treg cells （

0.01）， high percentage of Th17 cells （

0.01）， and high levels of IL-6 and STAT3 proteins in colon tissue （

0.01）. Compared with the model group， the administration groups showed alleviation of colon injury， high percentage of spleen Treg cells （

0.05，

0.01）， low percentage of Th17 cells （

0.01）， and low levels of IL-6 and STAT3 proteins in colon tissue （

0.01）.

Conclusion

Shaoyaotang regulates the balance of Th17/Treg by inhibiting the IL-6/STAT3 pathway， thereby relieving the pathological damage of UC rats with internal dampness-heat accumulation syndrome and affecting their immune function.

关键词

芍药汤湿热内蕴证溃疡性结肠炎白细胞介素-6（IL-6）/信号传导及转录激活因子3（STAT3）信号通路辅助性T细胞17（Th17）/调节性T细胞（Treg）平衡

Keywords

Shaoyaotanginternal dampness-heat accumulation syndromeulcerative colitisinterleukin-6 （IL-6）/signal transducer and activator of transcription 3 （STAT3） signaling pathwayT helper 17 （Th17）/Treg balance

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